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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
16/11/2010 |
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Data da última atualização: |
10/12/2010 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ZEID, M.; YU, J. K.; GOLDOWITZ, I.; DENTON, M. E.; COSTICH, D. E.; JAYASURIYA, C. T.; SAHA, M.; ELSHIRE, R.; BENSCHER, D.; BRESEGHELLO, F.; MUNKVOLD, J.; VARSHNEY, R. K.; BELAY, G.; SORRELLS, M. E. |
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Afiliação: |
M. ZEID, CORNELL UNIVERSITY; J. K. YU, CORNELL UNIVERSITY; I. GOLDOWITZ, CORNELL UNIVERSITY; M. E. DENTON, CORNELL UNIVERSITY; DENISE E. COSTICH, CORNELL UNIVERSITY; C. T. JAYASURIYA, CORNELL UNIVERSITY; M. SAHA, THE SAMUEL ROBERTS NOBLE FOUNDATION; R. ELSHIRE, CORNELL UNIVERSITY; D. BENSCHER, CORNELL UNIVERSITY; FLAVIO BRESEGHELLO, CNPAF; J. MUNKVOLD, BOYCE THOMPSON INSTITUTE; R. K. VARSHNEY, ICRISAT; G. BELAY, ETHIOPIAN INSTITUTE OF AGRICULTURAL RESEARCH; M. E. SORRELLS, CORNELL UNIVERSITY. |
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Título: |
Cross-amplification of EST-derived markers among 16 grass species. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Field Crops Research, Amsterdam, v. 118, n. 1, p. 28-35, Jul. 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
The availability of a large number of expressed sequence tags (ESTs) has facilitated the development of molecular markers in members of the grass family. As these markers are derived from coding sequences, cross-species amplification and transferability is higher than for markers designed from genomic DNA sequences. In this study, 919 EST-based primers developed from seven grass species were assessed for their amplification across a diverse panel of 16 grass species including cereal, turf and forage crops. Out of the 919 primers tested, 89 successfully amplified DNA from one or more species and 340 primers generated PCR amplicons from at least half of the species in the panel. Only 5.2% of the primers tested produced clear amplicons in all 16 species. The majority of the primers (66.9%) were developed from tall fescue and rice and these two species showed amplification rate of 41.6% and 19.0% across the panel, respectively. The highest amplification rate was found for conserved-intron scanning primers (CISP) developed from pearl millet (91%) and sorghum (75%) EST sequences that aligned to rice sequences. The primers with successful amplification identified in this study showed promise in other grass species as demonstrated in differentiating a set of 13 clones of reed canary grass, a species for which very little genomic research has been done. Sequences from the amplified PCR fragments indicated the potential for the transferable CISP markers for comparative mapping purposes. These primer sets can be immediately used for within and across species mapping and will be especially useful for minor grass species with few or no available molecular markers. MenosThe availability of a large number of expressed sequence tags (ESTs) has facilitated the development of molecular markers in members of the grass family. As these markers are derived from coding sequences, cross-species amplification and transferability is higher than for markers designed from genomic DNA sequences. In this study, 919 EST-based primers developed from seven grass species were assessed for their amplification across a diverse panel of 16 grass species including cereal, turf and forage crops. Out of the 919 primers tested, 89 successfully amplified DNA from one or more species and 340 primers generated PCR amplicons from at least half of the species in the panel. Only 5.2% of the primers tested produced clear amplicons in all 16 species. The majority of the primers (66.9%) were developed from tall fescue and rice and these two species showed amplification rate of 41.6% and 19.0% across the panel, respectively. The highest amplification rate was found for conserved-intron scanning primers (CISP) developed from pearl millet (91%) and sorghum (75%) EST sequences that aligned to rice sequences. The primers with successful amplification identified in this study showed promise in other grass species as demonstrated in differentiating a set of 13 clones of reed canary grass, a species for which very little genomic research has been done. Sequences from the amplified PCR fragments indicated the potential for the transferable CISP markers for comparative mapping purpose... Mostrar Tudo |
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Palavras-Chave: |
CISP; EST-SSR. |
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Thesagro: |
Genética molecular; Gramínea; Marcador molecular. |
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Thesaurus Nal: |
Poaceae; Sequence homology. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02615naa a2200361 a 4500
001 1867036
005 2010-12-10
008 2010 bl uuuu u00u1 u #d
100 1 $aZEID, M.
245 $aCross-amplification of EST-derived markers among 16 grass species.
260 $c2010
520 $aThe availability of a large number of expressed sequence tags (ESTs) has facilitated the development of molecular markers in members of the grass family. As these markers are derived from coding sequences, cross-species amplification and transferability is higher than for markers designed from genomic DNA sequences. In this study, 919 EST-based primers developed from seven grass species were assessed for their amplification across a diverse panel of 16 grass species including cereal, turf and forage crops. Out of the 919 primers tested, 89 successfully amplified DNA from one or more species and 340 primers generated PCR amplicons from at least half of the species in the panel. Only 5.2% of the primers tested produced clear amplicons in all 16 species. The majority of the primers (66.9%) were developed from tall fescue and rice and these two species showed amplification rate of 41.6% and 19.0% across the panel, respectively. The highest amplification rate was found for conserved-intron scanning primers (CISP) developed from pearl millet (91%) and sorghum (75%) EST sequences that aligned to rice sequences. The primers with successful amplification identified in this study showed promise in other grass species as demonstrated in differentiating a set of 13 clones of reed canary grass, a species for which very little genomic research has been done. Sequences from the amplified PCR fragments indicated the potential for the transferable CISP markers for comparative mapping purposes. These primer sets can be immediately used for within and across species mapping and will be especially useful for minor grass species with few or no available molecular markers.
650 $aPoaceae
650 $aSequence homology
650 $aGenética molecular
650 $aGramínea
650 $aMarcador molecular
653 $aCISP
653 $aEST-SSR
700 1 $aYU, J. K.
700 1 $aGOLDOWITZ, I.
700 1 $aDENTON, M. E.
700 1 $aCOSTICH, D. E.
700 1 $aJAYASURIYA, C. T.
700 1 $aSAHA, M.
700 1 $aELSHIRE, R.
700 1 $aBENSCHER, D.
700 1 $aBRESEGHELLO, F.
700 1 $aMUNKVOLD, J.
700 1 $aVARSHNEY, R. K.
700 1 $aBELAY, G.
700 1 $aSORRELLS, M. E.
773 $tField Crops Research, Amsterdam$gv. 118, n. 1, p. 28-35, Jul. 2010.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 16 | |
| 3. |  | PIROTA, R. D. P. B.; TONELOTTO, M.; DELABONA, P. da S.; POLIKARPOV, I.; FARINAS, C. S. Comparison of fpase and xylanase production between fungi isolated from amazon region and aspergillus niger (F12). In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programas e resumos... [Brasília, DF]: Sociedade Brasileira de Biotecnologia, 2010. p. 327. res. 411.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 4. | | PIROTA, R. D. P. B.; TONELOTTO, M.; DELABONA, P. da S.; POLIKARPOV, I.; FARINAS, C. S. Produção enzimática por fungos isolados da Região Amazônica, visando aplicação na produção de etanol celulósico. In: SEMINÁRIO BRASILEIRO DE TECNOLOGIA ENZIMÁTICA - ENZITEC, 9., 2010, Rio de Janeiro. Versatilidade e eficiência na inovação sustentável: resumos. Rio de Janeiro: UFRJ, 2010. p. 106.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 5. | | PIROTA, R. D. P. B.; DELABONA, P. da S.; POLIKARPOV, I.; FARINAS, C. S. Produção de carboximetilcelulase e poligalacturonase sólido pelo fungo Trichoderma polysporum por fermentação em estado sólido, utilizando bagaço de laranja com substrato. In: JORNADA CIENTÍFICA-EMBRAPA SÃO CARLOS, 2009, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste: Embrapa Instrumentação Agropecuária, 2009. p. 11 (Embrapa Pecuária Sudeste. Documentos, 90)| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 6. | | LEITE, F. L.; ALVES, W. F.; OLIVEIRA NETO, M.; POLIKARPOV, I.; HERRMANN, P. S. P.; MATTOSO, L. H. C.; OLIVEIRA, JUNIOR, O. N. Doping in poly (o-ethoxyaniline) nanostructured films studied with atomic force spectroscopy (AFS). Micron, New York, v. 39, p. 1119-1125, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Instrumentação. |
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| 7. | | LEITE, F. L.; OLIVEIRA NETO, M.; BALLESTERO, M. R. M.; POLIKARPOV, I.; MASCARENHAS, Y. P.; HERRMANN, P. S. P.; MATTOSO, L. H. C.; OLIVEIRA JUNIOR, O. N. Determination of conformation and fractal behavior on poly(0-ethoxyaniline) by atomic force microscopy and small-angle X-Ray scattering. In: MACRO 2006; WORLD POLYMER CONGRESS, Rio de Janeiro, Brazil; INTERNATIONAL SYMPOSIUM ON MACROMOLECULES, 41ST., 2006, Rio de Janeiro. Proceedings... [S. l.]: ABPol, 2006. 1 CD-ROM.| Biblioteca(s): Embrapa Instrumentação. |
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| 8. | | TONELOTTO, M. PIROTA, R. D. P. B. DELABONA, P. S. BARROS, G. O. F.; GOLUBEV, A. M. POLIKARPOV, I.; FARINAS, C. S. Isolation and characterization of a ß-galactosidase from a now Amazon forest strain of Aspergillus niger as a potential accessory enzyme for biomass conversion. Biocatalysis and Biotransformation, [S. l.], v. 32, n. 1, p. 13-22, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 9. | | ALESSI, A. M.; BIRD, S. M.; OATES, N. C.; LI, Y.; DOWLE, A. A.; NOVOTNY, E. H.; AZEVEDO, E. R. de; BENNETT, J. P.; POLIKARPOV, I.; YOUNG, J. P. W.; MCQUEEN-MASON, S. J.; BRUCE, N. C. Defining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies. Biotechnology for Biofuels, v. 11, article 166, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Solos. |
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| 10. | | LIMA, M. A.; GOMEZ, L. D.; STEELI-KING, C. G.; SIMISTER, R.; BERNARDINELLI, O. D.; CARVALHO, M. A.; REZENDE, C. A.; LABATE, C. A.; AZEVEDO, E. R.; McQUEEN-MASON, S.; POLIKARPOV, I.; CARVALHO, M. A. Evaluating the composition and processing potential of novel sources of Brazilian biomass for sustainable biorenewables production. Biotechnology for Biofuels, v. 7, n. 10, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Cerrados. |
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| 11. | | COLUSSI, F.; SERPA, V.; DELABONA, P. da S.; MANZINE, L. R.; VOLTATÓDIO, M. L.; ALVES, R.; MELLO, B. L.; PEREIRA JUNIOR, N.; FARINAS, C. S.; GOLUBEV, A. M.; SANTOS, M. A. M.; POLIKARPOV, I. Purification, and biochemical and biophysical characterization of cellobiohydrolase I from trichoderma harzianum IOC 3844. Journal of microbiology and biotechnology, Kangnam-Gu, v. 21, n. 8, p. 808-817, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 12. | | LEITE, F. de L.; SIMÕES, M. L.; OLIVEIRA NETO, M. de; SAVEDRA, R. M. L.; HERRMANN JUNIOR, P. S. de P.; POLIKARPOV, I.; MARTIN NETO, L.; TRSIC, M.; MATTOSO, L. H. C.; OLIVEIRA JUNIOR, O. N. de. A Nanocarrier charge model for the transport in poly(o-alkoxyanilines) [PTu19]. In: INTERNATIONAL CONFERENCE ON SCIENCE AND TECHNOLOGY OF SYNTHETIC METALS, ICSM, 2008, Porto de Galinhas. Book of Abstracts... [S. l.: s. n.], 2008. p. 61.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 13. | | HERRMANN, P. S. de P.; MASCARENHAS, Y. P.; POLIKARPOV, I.; BALLESTERO, M. R. M.; OLIVEIRA NETO, M. de; LEITE, F. de L.; MATTOSO, L. H. C.; OLIVEIRA, O. N. de. Nanoscale conformational ordering on poly(alkoxyanilines): fractal behavior and thin films formation mechanism. In: ENCONTRO DA SBPMAT, 5.; BRAZILIAN MRS MEETING, 5TH., 2006, Florianópolis. Final program... Florianópolis: [s. n.], 2006. p. 111. 1CD-ROM.| Biblioteca(s): Embrapa Instrumentação. |
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| 14. | | LEITE, F. L.; OLIVEIRA NETO, M. de; PATERNO, L. G.; BALLESTERO, M. R. M.; POLIKARPOV, I.; MASCARENHAS, Y. P.; HERRMANN, P. S. P.; MATTOSO, L. H. C.; OLIVEIRA JUNIOR, O. N. Nanoscale conformational ordering in polyanilines investigated by SAXS and AFM Journal of Colloid and Interface Science, New York, v. 316, p. 376-387, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Instrumentação. |
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| 15. | | ESPÍRITO SANTO, M. C. do; CARDOSO, E. B.; GUIMARAES, F. E. G.; AZEVEDO, E. R. de; CUNHA, G. P. da; NOVOTNY, E. H.; PELLEGRINI, V. de O. A.; CHANDEL, A. K.; SILVEIRA, M. H. L.; POLIKARPOV, I. Multifaceted characterization of sugarcane bagasse under different steam explosion severity conditions leading to distinct enzymatic hydrolysis yields. Industrial Crops & Products, v. 139, 111542, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Solos. |
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| 16. | | ESPIRITO SANTO, M. C. do; THEMA, F. T.; PELLEGRINI, V. de O. A.; KANE, A. O.; GUIMARAES, F. E. G.; FILGUEIRAS, J. G.; NOVOTNY, E. H.; AZEVEDO, E. R. de; POLIKARPOV, I. When the order matters: impacts of lignin removal and xylan conformation on the physical structure and enzymatic hydrolysis of sugarcane bagasse. Industrial Crops and Products, v. 180, 114708, Jun. 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Solos. |
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| Registros recuperados : 16 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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