Data/hora: 09/06/2024 05:41:57

Biblioteca(s): | Embrapa Trigo.
Data corrente: | 21/03/2017
Data da última atualização: | 17/08/2021
Autoria: | POERSCH-BORTOLON, L. B.; SCAGLIUSI, S. M. M.; YAMAZAKI-LAU, E.; BODANESE-ZANETTINI, M. H.
Afiliação: | LIANE BALVEDI POERSCH-BORTOLON, UFRGS; SANDRA MARIA MANSUR SCAGLIUSI, CNPT; ELENE YAMAZAKI LAU, CNPT; MARIA HELENA BODANESE-ZANETTINI, UFRGS.
Título: | Androgenic response of brazilian wheat genotypes to different pretreatments of spikes and to a gellinhg agent.
Ano de publicação: | 2016
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, DF, v. 51, n. 11, p. 1839-1847, nov. 2016.
Idioma: | Inglês
Conteúdo: | The objective of this work was to analyze the androgenic response of Brazilian wheat genotypes to different pretreatments of the spikes, prior to the culture of isolated microspores, and to the effect of a gelling agent in the induction culture medium. Five genotypes were evaluated for embryo formation, green plant regeneration, and spontaneous chromosome duplication. Wheat spikes were subjected to two pretreatments: cold, at 4°C for 21 days; and 2-hydroxynicotinic acid, at 32°C for two days. Culture media were evaluated with or without Ficoll as a gelling agent. Cold produced more embryos and green plants than the chemical pretreatment in four out of five genotypes. Only two genotypes treated with 2-hydroxynicotinic acid were able to produce plants, and one of them produced a single albino plant. Medium containing Ficoll produced more embryos than liquid medium and promoted a higher number of plants. Spontaneous chromosome duplication varies between genotypes and pretreatments, and shows high variability.
Palavras-Chave: | Albinismo; Androgênese; Cultura de micrósporo isolado; Duplo-haploid; Isolated microspore culture; Recalcitrance; Recalcitrância.
Thesagro: | Triticum Aestivum.
Thesaurus Nal: | Albino; Androgenesis; Doubled haploids.
Categoria do assunto: | G Melhoramento Genético
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/152460/1/Androgenic-response-of-brazilian.pdf
Registro original: | Embrapa Trigo (CNPT)
Exemplares: | CNPT - (43989-1) - UPC - AP - DD -  -  -  - DIS