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| Registros recuperados : 17 | |
| 6. |  | FASANELLA, C. C.; DIAS, A. C. F.; MELO, I. S. de; LUVIZOTTO, D.; PIZZIRANI-KLEINER, A. A.; DINI-ANDREOTE, F. Diversidade e funcionalidade fúngica na biorremediação de manguezais contaminados com petróleo. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 26., 2011, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Microbiologia, 2011. Resumo 417-1.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 8. | | SEBASTIANES, F. L. S.; LACAVA, P. T.; FAVARO, L. C. de L.; RODRIGUES, M. B. C.; ARAÚJO, W. L.; AZEVEDO, J. L.; PIZZIRANI-KLEINER, A. A. Genetic transformation of Diaporthe phaseolorum, an endophytic fungus found in mangrove forests, mediated by Agrobacterium tumefaciens. Current Genetics, Dec. 2011. Não paginado. DOI 10.1007/s00294-011-0362-2.| Biblioteca(s): Embrapa Agroenergia. |
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| 9. | | ANDREOTE, F. D.; DIAS, A. C. F.; FASANELLA, C. C.; TAKETANI, R. G.; ÁVILA, L. A.; PIZZIRANI-KLEINER, A. A.; AZEVEDO, J. L.; MELO, I. S. de. Microbial communities shifting in mangroves under distinct pollution state might name candidates for bioremediation programs. In: WORKSHOP DE BIODEGRADAÇÃO E BIORREMEDIAÇÃO, 3., 2009, Campinas. Anais... Jaguariúna: Embrapa Meio Ambiente, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 10. | | RODRIGUES, M. B. C.; ANDREOTE, F. D.; SPÓSITO, M. B.; AGUILLAR-VILDOSO, C. I.; ARAÚJO, W. L.; PIZZIRANI-KLEINER, A.A. Resistência a benzimidazóis por Guignardia citricarpa. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 3, p. 323-327, mar. 2007 Título em inglês: Guignardia citricarpa resistance to benzimidazoles.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 11. | | SEBASTIANES, F. L. S; ROMÃO-DUMARESQ, A. S.; LACAVA, P. T.; HARAKAVA, R.; AZEVEDO, J. L.; MELO, I. S. de; PIZZIRANI-KLEINER, A. A. Species diversity of culturable endophytic fungi from Brazilian mangrove forests. Current Genetics, Heidelberg, v. 59, n. 3, p. 153-166, 2013.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 12. | | Quecine, M. C.; Araújo, W. L.; Rosseto, P. B.; FERREIRA, A.; TSUI, S.; Lacava, P. T.; Mondin, M.; Azevedo, J. L.; Pizzirani-Kleiner, A. A. Sugarcane growth promotion by the endophytic bacterium Pantoea agglomerans 33.1. Applied and Environmental Microbiology, v. 78, n. 21, p. 7511-7518, 2012.| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 13. | | RODRIGUES, M. B. C.; FAVARO, L. C. de L.; PALLU, A. P. de S.; FERREIRA, A.; SEBASTIANES, F. de S.; RODRIGUES, M. J. C.; SPÓSITO, M. B.; ARAÚJO, W. L. de; PIZZIRANI-KLEINER, A. A. Agrobacterium-mediated transformation of Guignardia citricarpa: an efficient tool to gene transfer and random mutagenesis. Fungal Biology, Oxford, v. 117, n. 7/8, p. 556-568, jul./aug., 2013.| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 14. | | RODRIGUES, M. B. C.; FAVARO, L. C. de L.; PALUU, A. P. de S.; FERREIRA, A.; SEBASTIANES, F. de S.; RODRIGUES, M. J. C.; SPOSITO, M. B.; ARAUJO, W. L. de; PIZZIRANI-KLEINER, A. A. Agrobacterium-mediated transformation of Guignardia citricarpa: an efficient tool to gene transfer and random mutagenesis. Fungal Biology, v. 117, n. 7-8, p. 463-576, 2013.| Biblioteca(s): Embrapa Agroenergia. |
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| 15. | | RODRIGUES, M. B. C.; FAVARO, L. C. de L.; PALLU, A. P. S.; FERREIRA, A.; SEBASTIANES, F. L. S.; RODRIGUES, M. J. C.; SPÓSITO, M. B.; ARAÚJO, W. L.; BOGAS, A. C.; PIZZIRANI-KLEINER, A. A. Guignardia citricarpa: Agrobacterium-mediated transformation and functional GFP expression. In: CONGRESS OF EUROPEAN MICROBIOLOGISTS - FEMS, 4., 2011, Geneva, Switzerland. [s.L.]: Kenes Group, 2011. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
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| 16. | | ALMEIDA, J. R. M. de; FRANCO, P. F.; POLETTO, C. M.; MENDES, T. D.; SALUM, T. F. C.; ARAUJO, W. A.; PIZZIRANI-KLEINER, A. A.; FAVARO, L. C. de L.; LOPES, L. G. New yeast strains for production of fuels and chemicals. In: INTERNATIONAL CONGRESS ON YEASTS, 2012, Madison, US. [Anais...]. [S.l.]: Lesaffre, 2012.| Biblioteca(s): Embrapa Agroenergia. |
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| 17. | | FASANELLA, C. C.; DIAS, A. C. F.; RIGONATO, J.; FIORE, M. F.; SOARES JUNIOR, F. L.; MELO, I. S. de; PIZZIRANI-KLEINER, A. A.; ELSAS, J. D. van; ANDREOTE, F. D. The selection exerted by oil contamination on mangrove fungal communities. Water, Air and Soil Pollution, Dordrecht, v. 223, n. 7, p. 4233-4243, 2012.| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 17 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agrossilvipastoril. |
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Data corrente: |
03/02/2014 |
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Data da última atualização: |
10/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
RODRIGUES, M. B. C.; FAVARO, L. C. de L.; PALLU, A. P. de S.; FERREIRA, A.; SEBASTIANES, F. de S.; RODRIGUES, M. J. C.; SPÓSITO, M. B.; ARAÚJO, W. L. de; PIZZIRANI-KLEINER, A. A. |
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Afiliação: |
Maria Beatriz Calderan Rodrigues, USP-ESALQ; LEIA CECILIA DE LIMA FAVARO, CNPAE; Ana Paula de Souza Pallu, USP-ESALQ; ANDERSON FERREIRA, CPAMT; Fernanda de Souza Sebastianes, USP-ESALQ; Maria Juliana Calderan Rodrigues, USP-ESALQ; Marcel Bellato Spósito, USP-ESALQ; Welington Luiz DE Araújo, UMC; Aline Aparecida Pizzirani-Kleiner, USP-ESALQ. |
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Título: |
Agrobacterium-mediated transformation of Guignardia citricarpa: an efficient tool to gene transfer and random mutagenesis. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Fungal Biology, Oxford, v. 117, n. 7/8, p. 556-568, jul./aug., 2013. |
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ISSN: |
1878-6146 |
|
DOI: |
http://dx.doi.org/10.1016/j.funbio.2013.06.005 |
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Idioma: |
Inglês |
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Conteúdo: |
Guignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control CBS. This work describes for the first time an efficient and successful methodology for genetic transformation of G. citricarpa mycelia, which generated transformants expressing the gene encoding for the gfp (green fluorescent protein) and also their interaction with citrus plant. Mycelia of G. citricarpa were transformed via Agrobacterium tumefaciens, which carried the plasmid pFAT-gfp, contains the genes for hygromycin resistance (hph) as well as gfp. The optimization of the agrotransformation protocol was performed testing different conditions (type of membrane; inductor agent concentration [acetosyringonee - AS] and cocultivation time). Results demonstrated that the best condition occurred with the utilization of cellulose?s ester membrane; 200µM of AS and 96 h as cocultivation time. High mitotic stability (82 %) was displayed by transformants using Polymerase Chain Reaction (PCR) technique to confirm the hph gene insertion. In addition, the presence of gfp was observed inside mycelia by epifluorescence optical microscopy. This technique easy visualization of the behaviour of the pathogen interacting with the plant for the first time, allowing future studies on the pathogenesis of this fungus. The establishment of a transformation method for G. citricarpa opens a range of possibilities and facilitates the study of insertional mutagenesis and genetic knockouts, in order to identify the most important genes involved in the pathogenesis mechanisms and plant-pathogen interaction. MenosGuignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control CBS. This work describes for the first time an efficient and successful methodology for genetic transformation of G. citricarpa mycelia, which generated transformants expressing the gene encoding for the gfp (green fluorescent protein) and also their interaction with citrus plant. Mycelia of G. citricarpa were transformed via Agrobacterium tumefaciens, which carried the plasmid pFAT-gfp, contains the genes for hygromycin resistance (hph) as well as gfp. The optimization of the agrotransformation protocol was performed testing different conditions (type of membrane; inductor agent concentration [acetosyringonee - AS] and cocultivation time). Results demonstrated that the best condition occurred with the utilization of cellulose?s ester membrane; 200µM of AS and 96 h as cocultivation time. High mitotic stability (82 %) was displayed by transformants using Polymerase Chain Reaction (PCR) technique to confirm the hph gene insertion. In addition, the presence of gfp was observed inside mycelia by epifluorescence optical microscopy. This technique easy visualization of the behaviour of the pathogen interacting with the plant for ... Mostrar Tudo |
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Palavras-Chave: |
Agrobacterium-Mediated; Plant-Pathogen Interaction. |
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Thesaurus NAL: |
citrus black spot; genetic transformation; Guignardia citricarpa. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/96268/1/cpamt-Ferreira-1878-6146-2013.pdf
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Marc: |
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520 $aGuignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control CBS. This work describes for the first time an efficient and successful methodology for genetic transformation of G. citricarpa mycelia, which generated transformants expressing the gene encoding for the gfp (green fluorescent protein) and also their interaction with citrus plant. Mycelia of G. citricarpa were transformed via Agrobacterium tumefaciens, which carried the plasmid pFAT-gfp, contains the genes for hygromycin resistance (hph) as well as gfp. The optimization of the agrotransformation protocol was performed testing different conditions (type of membrane; inductor agent concentration [acetosyringonee - AS] and cocultivation time). Results demonstrated that the best condition occurred with the utilization of cellulose?s ester membrane; 200µM of AS and 96 h as cocultivation time. High mitotic stability (82 %) was displayed by transformants using Polymerase Chain Reaction (PCR) technique to confirm the hph gene insertion. In addition, the presence of gfp was observed inside mycelia by epifluorescence optical microscopy. This technique easy visualization of the behaviour of the pathogen interacting with the plant for the first time, allowing future studies on the pathogenesis of this fungus. The establishment of a transformation method for G. citricarpa opens a range of possibilities and facilitates the study of insertional mutagenesis and genetic knockouts, in order to identify the most important genes involved in the pathogenesis mechanisms and plant-pathogen interaction.
650 $acitrus black spot
650 $agenetic transformation
650 $aGuignardia citricarpa
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773 $tFungal Biology, Oxford$gv. 117, n. 7/8, p. 556-568, jul./aug., 2013.
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