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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
29/10/2012 |
Data da última atualização: |
18/05/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LANA, U. G. de P.; GOMES, E. A.; SILVA, D. D. da; COSTA, R. V. da; COTA, L. V.; PARREIRA, D. F.; SOUZA, I. R. P.; GUIMARAES, C. T. |
Afiliação: |
UBIRACI GOMES DE PAULA LANA, CNPMS; ELIANE APARECIDA GOMES, CNPMS; DAGMA DIONISIA DA SILVA, CNPMS; RODRIGO VERAS DA COSTA, CNPMS; LUCIANO VIANA COTA, CNPMS; ISABEL REGINA PRAZERES DE SOUZA, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS. |
Título: |
Detection and molecular diversity of Pantoea ananatis associated with white spot disease in maize, sorghum and crabgrass in Brazil. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Journal of Phytopathology, Berlin, v. 160, p. 441-448, 2012. |
DOI: |
10.1111/j.1439-0434.2012.01924.x |
Idioma: |
Inglês |
Conteúdo: |
Bacteria of the genus Pantoea have become important plant pathogens worldwide in recent years. Pantoea ananatis was reported as the cause of maize white spot, a serious maize disease in Brazil, causing significant yield losses. However, very little information is available about how to detect this pathogen, its genetic variability and the putative alternative hosts in maize-growing areas. To address these issues, we implemented a rapid and efficient PCR-based method to identify P. ananatis isolated from leaves showing white spot symptoms and evaluated its genetic diversity in maize, sorghum and crabgrass. Of the 29 bacteria isolated from typical water-soaked lesions of white spot disease that produced yellow colonies, 15 isolates were identified as P. ananatis by 16S rDNA sequencing and correctly detected by the PCR reaction, amplifying a specific fragment of the ice nucleation gene (ina). These P. ananatis isolates included 13 from maize, one from sorghum and one from crabgrass, while the other 14 yellow colony isolates were from other bacterial species, including two Pantoea species (Pantoea dispersa and Pantoea agglomerans) that were not amplified by the ina primers. These results indicate that the optimized PCR assay can be used to detect P. ananatis isolated from white spot lesions and could be used as a large-scale and cost-effective method of detecting this pathogen in leaf lesions on maize and other grasses. All isolates were evaluated for hypersensitive response (HR) on tobacco, revealing that some P. ananatis were able to induce HR. The high genetic variability revealed by rep-PCR did not differentiated the P. ananatis isolates based on their hosts or HR reaction. The detection, characterization and diversity of P.ananatis from maize, sorghum and crabgrass in our study can be applied in understanding epidemiology and designing control strategies for maize white spot disease in Brazil. MenosBacteria of the genus Pantoea have become important plant pathogens worldwide in recent years. Pantoea ananatis was reported as the cause of maize white spot, a serious maize disease in Brazil, causing significant yield losses. However, very little information is available about how to detect this pathogen, its genetic variability and the putative alternative hosts in maize-growing areas. To address these issues, we implemented a rapid and efficient PCR-based method to identify P. ananatis isolated from leaves showing white spot symptoms and evaluated its genetic diversity in maize, sorghum and crabgrass. Of the 29 bacteria isolated from typical water-soaked lesions of white spot disease that produced yellow colonies, 15 isolates were identified as P. ananatis by 16S rDNA sequencing and correctly detected by the PCR reaction, amplifying a specific fragment of the ice nucleation gene (ina). These P. ananatis isolates included 13 from maize, one from sorghum and one from crabgrass, while the other 14 yellow colony isolates were from other bacterial species, including two Pantoea species (Pantoea dispersa and Pantoea agglomerans) that were not amplified by the ina primers. These results indicate that the optimized PCR assay can be used to detect P. ananatis isolated from white spot lesions and could be used as a large-scale and cost-effective method of detecting this pathogen in leaf lesions on maize and other grasses. All isolates were evaluated for hypersensitive response (HR... Mostrar Tudo |
Palavras-Chave: |
Diversidade genética. |
Thesagro: |
Doença de planta; Mancha branca. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02743naa a2200253 a 4500 001 1938414 005 2017-05-18 008 2012 bl uuuu u00u1 u #d 024 7 $a10.1111/j.1439-0434.2012.01924.x$2DOI 100 1 $aLANA, U. G. de P. 245 $aDetection and molecular diversity of Pantoea ananatis associated with white spot disease in maize, sorghum and crabgrass in Brazil.$h[electronic resource] 260 $c2012 520 $aBacteria of the genus Pantoea have become important plant pathogens worldwide in recent years. Pantoea ananatis was reported as the cause of maize white spot, a serious maize disease in Brazil, causing significant yield losses. However, very little information is available about how to detect this pathogen, its genetic variability and the putative alternative hosts in maize-growing areas. To address these issues, we implemented a rapid and efficient PCR-based method to identify P. ananatis isolated from leaves showing white spot symptoms and evaluated its genetic diversity in maize, sorghum and crabgrass. Of the 29 bacteria isolated from typical water-soaked lesions of white spot disease that produced yellow colonies, 15 isolates were identified as P. ananatis by 16S rDNA sequencing and correctly detected by the PCR reaction, amplifying a specific fragment of the ice nucleation gene (ina). These P. ananatis isolates included 13 from maize, one from sorghum and one from crabgrass, while the other 14 yellow colony isolates were from other bacterial species, including two Pantoea species (Pantoea dispersa and Pantoea agglomerans) that were not amplified by the ina primers. These results indicate that the optimized PCR assay can be used to detect P. ananatis isolated from white spot lesions and could be used as a large-scale and cost-effective method of detecting this pathogen in leaf lesions on maize and other grasses. All isolates were evaluated for hypersensitive response (HR) on tobacco, revealing that some P. ananatis were able to induce HR. The high genetic variability revealed by rep-PCR did not differentiated the P. ananatis isolates based on their hosts or HR reaction. The detection, characterization and diversity of P.ananatis from maize, sorghum and crabgrass in our study can be applied in understanding epidemiology and designing control strategies for maize white spot disease in Brazil. 650 $aDoença de planta 650 $aMancha branca 653 $aDiversidade genética 700 1 $aGOMES, E. A. 700 1 $aSILVA, D. D. da 700 1 $aCOSTA, R. V. da 700 1 $aCOTA, L. V. 700 1 $aPARREIRA, D. F. 700 1 $aSOUZA, I. R. P. 700 1 $aGUIMARAES, C. T. 773 $tJournal of Phytopathology, Berlin$gv. 160, p. 441-448, 2012.
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Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
13/05/2005 |
Data da última atualização: |
10/05/2022 |
Autoria: |
IGUMA, L. T.; LISAUSKAS, S. F. C.; MELO, E. O.; FRANCO, M. M.; PIVATO, I.; VIANNA, G. R.; SOUSA, R. V.; DODE, M. A. N.; ARAGÃO, F. J. L.; RECH, E. L.; RUMPF, R. |
Título: |
Development of bovine embryos reconstructed by nuclear transfer of transfected and non-transfected adult fibroblast cells. |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 4, n. 1, p. 55-66, 2005. |
Idioma: |
Inglês |
Palavras-Chave: |
Fibroplasto; Tecnologia de reprodução assistida. |
Thesagro: |
Animal Transgênico; Bovino. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/185653/1/gmr0142.pdf
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Marc: |
LEADER 00850naa a2200277 a 4500 001 1185653 005 2022-05-10 008 2005 bl uuuu u00u1 u #d 100 1 $aIGUMA, L. T. 245 $aDevelopment of bovine embryos reconstructed by nuclear transfer of transfected and non-transfected adult fibroblast cells.$h[electronic resource] 260 $c2005 650 $aAnimal Transgênico 650 $aBovino 653 $aFibroplasto 653 $aTecnologia de reprodução assistida 700 1 $aLISAUSKAS, S. F. C. 700 1 $aMELO, E. O. 700 1 $aFRANCO, M. M. 700 1 $aPIVATO, I. 700 1 $aVIANNA, G. R. 700 1 $aSOUSA, R. V. 700 1 $aDODE, M. A. N. 700 1 $aARAGÃO, F. J. L. 700 1 $aRECH, E. L. 700 1 $aRUMPF, R. 773 $tGenetics and Molecular Research$gv. 4, n. 1, p. 55-66, 2005.
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