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Registros recuperados : 722 | |
126. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SEBBENN, A. M.; PIRES, C. L. da S.; SALDANHA, H. X.; ZANATTO, A. C. S. Teste de progenies de polinizacao livre de Pinus tecunumanii (Eq. et Per.) Styles de San Rafael del Norte, na regiao de Sao Simao, SP. Revista do Instituto Florestal, Sao Paulo, v.7, n.2, p.241-252, 1995. Biblioteca(s): Embrapa Florestas. |
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129. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | TOLEDO FILHO, D. V. de; PIRES, C. L. da S. ROSA, P. R. F. da. Teste de origens de Pinus caribaea Mor. Silvicultura, São Paulo, v. 11, n. 41, p. 114, 1986. Resumo. Edição dos anais do 5º Congresso Florestal Brasileiro, 1986, Olinda. Usos múltiplos da floresta: uma necessidade. Biblioteca(s): Embrapa Florestas. |
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133. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | CARVALHO, R. M. V.; PIRES, C.; SCHMIDT, F.; LAUMANN, R. A. E.; SUJII, E. Ecologia de populações de crisomelídeos-praga aplicada ao uso de semioquímicos. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 7., 2002, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2002. P. 92. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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135. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | TOREZANI, K.; LAUMANN, R.; NORONHA, S. E. de; SUJII, E.; PIRES, C. Diversidade de abelhas em cultura de aboboreira Cucurbita Pepo L. no Distrito Federal: influência do sistema de produção e da paisagem agrícola. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 4., 2016, Curitiba. Recursos genéticos no Brasil: a base para o desenvolvimento sustentável: anais. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2016. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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137. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | WANDERLEY, R. da C.; CHAVES FILHO, N.; PIRES, C. B.; CÂMARA, U. Digestibilidade dos farelos de algodão e mamona para ruminantes. In: REUNIÃO DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 9., 1972, Viçosa, MG. Resumos dos trabalhos. Viçosa, MG: SBZ: UFV, 1972. p. 95-96. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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138. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | LIMA, M. G. P.; LARA, M. dos S.; CAMPOS, L. A. de; PIRES, C. C. S. Biossegurança da proteína CRY1AC, sintetizada pelo algodão geneticamente modificado, em abelhas africanizadas. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: anais. Viçosa, MG: UFV, 2008. 1 CD-ROM. ResumoID:2444-1. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 722 | |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
31/08/2015 |
Data da última atualização: |
20/06/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
PAULA, D. P.; LINARD, B.; ANDOW, D. A.; SUJII, E. R.; PIRES, C. S. S.; VOGLER, A. P. |
Afiliação: |
DEBORA PIRES PAULA, CENARGEN; BENJAMIN LINARD, NATURAL HISTORY MUSEUM; DAVID A. ANDOW, UNIVERSITY OF MINNESOTA; EDISON RYOITI SUJII, CENARGEN; CARMEN SILVIA SOARES PIRES, CENARGEN; ALFRIED P. VOGLER, NATURAL HISTORY MUSEUM. |
Título: |
Detection and decay rates of prey and prey symbionts in the gut of a predator through metagenomics. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Molecular Ecology Resources, v. 15, n. 4, p. 880-892, 2015. |
DOI: |
10.1111/1755-0998.12364 |
Idioma: |
Inglês |
Conteúdo: |
DNA methods are useful to identify ingested prey items from the gut of predators, but reliable detection is hampered by low amounts of degraded DNA. PCR-based methods can retrieve minute amounts of starting material but suffer from amplification biases and cross-reactions with the predator and related species genomes. Here, we use PCR-free direct shotgun sequencing of total DNA isolated from the gut of the harlequin ladybird Harmonia axyridis at five time points after feeding on a single pea aphid Acyrthosiphon pisum. Sequence reads were matched to three reference databases: Insecta mitogenomes of 587 species, including H. axyridis sequenced here; A. pisum nuclear genome scaffolds; and scaffolds and complete genomes of 13 potential bacterial symbionts. Immediately after feeding, multicopy mtDNA of A. pisum was detected in tens of reads, while hundreds of matches to nuclear scaffolds were detected. Aphid nuclear DNA and mtDNA decayed at similar rates (0.281 and 0.11 h1 respectively), and the detectability periods were 32.7 and 23.1 h. Metagenomic sequencing also revealed thousands of reads of the obligate Buchnera aphidicola and facultative Regiella insecticola aphid symbionts, which showed exponential decay rates significantly faster than aphid DNA (0.694 and 0.80 h 1, respectively). However, the facultative aphid symbionts Hamiltonella defensa, Arsenophonus spp. and Serratia symbiotica showed an unexpected temporary increase in population size by 1?2 orders of magnitude in the predator guts before declining. Metagenomics is a powerful tool that can reveal complex relationships and the dynamics of interactions among predators, prey and their symbionts. MenosDNA methods are useful to identify ingested prey items from the gut of predators, but reliable detection is hampered by low amounts of degraded DNA. PCR-based methods can retrieve minute amounts of starting material but suffer from amplification biases and cross-reactions with the predator and related species genomes. Here, we use PCR-free direct shotgun sequencing of total DNA isolated from the gut of the harlequin ladybird Harmonia axyridis at five time points after feeding on a single pea aphid Acyrthosiphon pisum. Sequence reads were matched to three reference databases: Insecta mitogenomes of 587 species, including H. axyridis sequenced here; A. pisum nuclear genome scaffolds; and scaffolds and complete genomes of 13 potential bacterial symbionts. Immediately after feeding, multicopy mtDNA of A. pisum was detected in tens of reads, while hundreds of matches to nuclear scaffolds were detected. Aphid nuclear DNA and mtDNA decayed at similar rates (0.281 and 0.11 h1 respectively), and the detectability periods were 32.7 and 23.1 h. Metagenomic sequencing also revealed thousands of reads of the obligate Buchnera aphidicola and facultative Regiella insecticola aphid symbionts, which showed exponential decay rates significantly faster than aphid DNA (0.694 and 0.80 h 1, respectively). However, the facultative aphid symbionts Hamiltonella defensa, Arsenophonus spp. and Serratia symbiotica showed an unexpected temporary increase in population size by 1?2 orders of magnitude in ... Mostrar Tudo |
Palavras-Chave: |
Analyte detectability half-life; Coccinellid. |
Thesagro: |
Pulgão. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/135237/1/Paula-et-al-2015-Molecular-Ecology-Resources.pdf
|
Marc: |
LEADER 02376naa a2200229 a 4500 001 2023012 005 2024-06-20 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1111/1755-0998.12364$2DOI 100 1 $aPAULA, D. P. 245 $aDetection and decay rates of prey and prey symbionts in the gut of a predator through metagenomics.$h[electronic resource] 260 $c2015 520 $aDNA methods are useful to identify ingested prey items from the gut of predators, but reliable detection is hampered by low amounts of degraded DNA. PCR-based methods can retrieve minute amounts of starting material but suffer from amplification biases and cross-reactions with the predator and related species genomes. Here, we use PCR-free direct shotgun sequencing of total DNA isolated from the gut of the harlequin ladybird Harmonia axyridis at five time points after feeding on a single pea aphid Acyrthosiphon pisum. Sequence reads were matched to three reference databases: Insecta mitogenomes of 587 species, including H. axyridis sequenced here; A. pisum nuclear genome scaffolds; and scaffolds and complete genomes of 13 potential bacterial symbionts. Immediately after feeding, multicopy mtDNA of A. pisum was detected in tens of reads, while hundreds of matches to nuclear scaffolds were detected. Aphid nuclear DNA and mtDNA decayed at similar rates (0.281 and 0.11 h1 respectively), and the detectability periods were 32.7 and 23.1 h. Metagenomic sequencing also revealed thousands of reads of the obligate Buchnera aphidicola and facultative Regiella insecticola aphid symbionts, which showed exponential decay rates significantly faster than aphid DNA (0.694 and 0.80 h 1, respectively). However, the facultative aphid symbionts Hamiltonella defensa, Arsenophonus spp. and Serratia symbiotica showed an unexpected temporary increase in population size by 1?2 orders of magnitude in the predator guts before declining. Metagenomics is a powerful tool that can reveal complex relationships and the dynamics of interactions among predators, prey and their symbionts. 650 $aPulgão 653 $aAnalyte detectability half-life 653 $aCoccinellid 700 1 $aLINARD, B. 700 1 $aANDOW, D. A. 700 1 $aSUJII, E. R. 700 1 $aPIRES, C. S. S. 700 1 $aVOGLER, A. P. 773 $tMolecular Ecology Resources$gv. 15, n. 4, p. 880-892, 2015.
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