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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
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Data corrente: |
11/04/2024 |
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Data da última atualização: |
11/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VAZ, C. S. L.; FONSECA, F. M. da; RECH, D. V.; MORES, M. A. Z.; COLDEBELLA, A.; CANTAO, M. E. |
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Afiliação: |
CLARISSA SILVEIRA LUIZ VAZ, CNPSA; FRANCISCO NOE DA FONSECA, GEM; DAIANE VOSS RECH, CNPSA; MARCOS ANTONIO ZANELLA MORES, CNPSA; ARLEI COLDEBELLA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA. |
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Título: |
Wild-type lytic bacteriophages against Salmonella Heidelberg: further characterization and effect of prophylactic therapy in broiler chickens. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Research in Veterinary Science, v. 171, n. 105247, 2024. |
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DOI: |
https://doi.org/10.1016/j.rvsc.2024.105247 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous administration of the phage suspension most likely induced the development of bacteriophage-resistant mutants, which might have affected the in vivo effect. Therefore, a putative administration protocol should be based on other strategies, such as short-term therapy at pre-harvest age. MenosAbstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous a... Mostrar Tudo |
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Palavras-Chave: |
Análise genômica; Bacteriófago; Foodborne pathogen; Genomic analysis; Patógeno de origem alimentar; Terapia fágica. |
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Thesagro: |
Frango de Corte; Salmonelose. |
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Thesaurus Nal: |
Bacteriophages; Phage therapy; Poultry; Salmonellosis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02834naa a2200337 a 4500
001 2163526
005 2024-04-11
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.rvsc.2024.105247$2DOI
100 1 $aVAZ, C. S. L.
245 $aWild-type lytic bacteriophages against Salmonella Heidelberg$bfurther characterization and effect of prophylactic therapy in broiler chickens.$h[electronic resource]
260 $c2024
520 $aAbstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous administration of the phage suspension most likely induced the development of bacteriophage-resistant mutants, which might have affected the in vivo effect. Therefore, a putative administration protocol should be based on other strategies, such as short-term therapy at pre-harvest age.
650 $aBacteriophages
650 $aPhage therapy
650 $aPoultry
650 $aSalmonellosis
650 $aFrango de Corte
650 $aSalmonelose
653 $aAnálise genômica
653 $aBacteriófago
653 $aFoodborne pathogen
653 $aGenomic analysis
653 $aPatógeno de origem alimentar
653 $aTerapia fágica
700 1 $aFONSECA, F. M. da
700 1 $aRECH, D. V.
700 1 $aMORES, M. A. Z.
700 1 $aCOLDEBELLA, A.
700 1 $aCANTAO, M. E.
773 $tResearch in Veterinary Science$gv. 171, n. 105247, 2024.
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Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
28/05/1998 |
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Data da última atualização: |
17/10/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
VASCONCELOS, V. R.; RESENDE, K. T.; PIMENTEL, J. C. M.; CARVALHO, F. F. R. de; RIBEIRO, V. Q.; DORIGAN, C. J. |
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Afiliação: |
VÂNIA RODRIGUES VASCONCELOS, CNPC; KLÉBER TOMÁS DE RESENDE, UNESP - Jaboticabal, SP; JOSÉ CARLOS MACHADO PIMENTEL, CNPC; FRANCISCO FERNANDO F. DE CARVALHO, Pós-graduação - UNESP - Jaboticabal, SP; VALDENIR QUEIROZ RIBEIRO, CNPC; CLÁUDIA JOSEFINA DORIGAN, Pós-graduação - UNESP - Jaboticabal, SP. |
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Título: |
Cinética de degradação ruminal da proteína de forrageiras do semi-árido brasileiro em caprinos. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 34., 1997, Juiz de Fora. Anais... Juiz de Fora: Sociedade Brasileira de Zootecnia, 1997. v. 1, p. 52-54. |
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Idioma: |
Português |
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Conteúdo: |
Resumo: Determinou-se as diferentes degradabilidades da proteina de acordo com suas atividades de degradacao e passagem calculadas segundo metodologia proposta pelo AFRC (1995), utilizando-se as fracoes soluveis(A), potencialmente degradavel(B), indegradavel(C) e a taxa de degradacao da fracao B (C), parametros da equacao de ORSKOV & McDONALD (1979). A concentracao de proteina bruta e do nitrogenio insoluvel em detergente acido foram determinados segundo SILVA (1990). Os fenos de sabia (Mimosa caesalpinifolia) e jurema preta (Mimosa atenuifolia) apresentaram baixo potencial de degradacao ruminal da PB e os fenos de catingueira (Caesalpinia bracteosa) e leucena (Leucaena leucocephala) percentuais elevados de proteina degradada no rumen, podendo ser usadas como suplementos proteicos na regiao semi-arida no Nordeste. [Cinetic of rumenal degradation of the protein of brazilian semi-arid forages in goats]. |
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Palavras-Chave: |
Brasil; Caesalpinia bracteosa; Ceará; Degradabilidade; Degradability; Mimosa atenuifolia; Mimosa caesalpinifolia; Protein. |
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Thesagro: |
Caprino; Catingueira; Feno; Jurema preta; Leucaena; Leucaena leucocephala; Proteina; Sabiá. |
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Thesaurus NAL: |
Brazil; Goats; Hay; Semiarid zones. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/48382/1/AAC-Cinetica-de-degradacao.pdf
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Marc: |
LEADER 02131nam a2200409 a 4500
001 1514952
005 2023-10-17
008 1997 bl uuuu u00u1 u #d
100 1 $aVASCONCELOS, V. R.
245 $aCinética de degradação ruminal da proteína de forrageiras do semi-árido brasileiro em caprinos.$h[electronic resource]
260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 34., 1997, Juiz de Fora. Anais... Juiz de Fora: Sociedade Brasileira de Zootecnia, 1997. v. 1, p. 52-54.$c1997
520 $aResumo: Determinou-se as diferentes degradabilidades da proteina de acordo com suas atividades de degradacao e passagem calculadas segundo metodologia proposta pelo AFRC (1995), utilizando-se as fracoes soluveis(A), potencialmente degradavel(B), indegradavel(C) e a taxa de degradacao da fracao B (C), parametros da equacao de ORSKOV & McDONALD (1979). A concentracao de proteina bruta e do nitrogenio insoluvel em detergente acido foram determinados segundo SILVA (1990). Os fenos de sabia (Mimosa caesalpinifolia) e jurema preta (Mimosa atenuifolia) apresentaram baixo potencial de degradacao ruminal da PB e os fenos de catingueira (Caesalpinia bracteosa) e leucena (Leucaena leucocephala) percentuais elevados de proteina degradada no rumen, podendo ser usadas como suplementos proteicos na regiao semi-arida no Nordeste. [Cinetic of rumenal degradation of the protein of brazilian semi-arid forages in goats].
650 $aBrazil
650 $aGoats
650 $aHay
650 $aSemiarid zones
650 $aCaprino
650 $aCatingueira
650 $aFeno
650 $aJurema preta
650 $aLeucaena
650 $aLeucaena leucocephala
650 $aProteina
650 $aSabiá
653 $aBrasil
653 $aCaesalpinia bracteosa
653 $aCeará
653 $aDegradabilidade
653 $aDegradability
653 $aMimosa atenuifolia
653 $aMimosa caesalpinifolia
653 $aProtein
700 1 $aRESENDE, K. T.
700 1 $aPIMENTEL, J. C. M.
700 1 $aCARVALHO, F. F. R. de
700 1 $aRIBEIRO, V. Q.
700 1 $aDORIGAN, C. J.
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Embrapa Caprinos e Ovinos (CNPC) |
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