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Registros recuperados : 13 | |
6. | | BEVITORI, R.; POPIELARSKA-KONIECZNA, M.; SANTOS, E. M. dos; GROSSI-DE-SÁ, M. F.; PETROFEZA, S. Morpho-anatomical characterization of mature embryo-derived callus of rice (Oryza sativa L.) suitable for transformation. Protoplasma, New York, v. 251, n. 3, p. 545-554, May 2014. Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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7. | | OLIVEIRA, C. M.; FREITAS, M. A.; FALEIRO, V. de O.; PETROFEZA, S. Estratégia moleculares para identificação de Meloidogyne incognita, M. javanica, Pratylenchus brachyurus e P. coffese In: CONGRESSO BRASILEIRO DE NEMATOLOGIA, 31., 2013, Cuiabá. Anais... Cuiabá, MT: Sociedade Brasileira de Nematologia, 2013. Biblioteca(s): Embrapa Agrossilvipastoril. |
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10. | | MAGALHÃES, L. F.; CUNHA, C. P. R.; LOBO JUNIOR, M.; PETROFEZA, S. Diversidade genética em populações de Sclerotinia sclerotiorum na cultura de feijoeiro (Phaseolus vulgaris L.). Tropical Plant Pathology, Brasília, DF, v. 36, p. 1124, ago. 2011. Suplemento, ref. 1576. Edição dos Resumos do 44 Congresso Brasileiro de Fitopatologia, Bento Gonçalves, ago. 2011. 1 CD-ROM. Biblioteca(s): Embrapa Arroz e Feijão. |
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11. | | OLIVEIRA, M. B.; SANTOS, E. M. dos; INOCÊNCIO, A. P. M.; CUNHA, C. P. R.; LOBO JUNIOR, M.; PETROFEZA, S. Análise de enimas hidrolíticas durante a interação do fungo Sclerotinia sclerotiorum e plantas de feijoeiro (Phaseolus vulgaris L.). In: CONGRESSO DE GENÉTICA DO CENTRO-OESTE, 2., 2010, Anais... Goiânia. Goiânia: Universidade Federal de Goiás, 2010. 1 CD-ROM. Biblioteca(s): Embrapa Arroz e Feijão. |
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12. | | BEVITORI, R.; OLIVEIRA, M. B.; GROSSI-DE-SÁ, M. F.; LANNA, A. C.; SILVEIRA, R. D. da; PETROFEZA, S. Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought. Genetics and Molecular Research, v. 13, n. 4, p. 9795-9805, 2014. Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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13. | | BARBOSA, S. R. C.; GOMES, E.; BRESEGHELO, L.; MOURA, A. L. D.; ALVES, E. B.; KNOB, A.; ALMEIDA, A. M. R.; LOBO JUNIOR, M.; PETROFEZA, S. Análise parcial do transcriptoma de Sclerotinia sclerotiorum durante a interação patogênica com feijão comum (Phaseolus vulgaris L.). In: CONGRESSO BRASILEIRO DE GENÉTICA, 52.; CONGRESO DE LA ASOCIACIÓN LATINOAMERICANA DE GENÉTICA, 12., 2006, Foz do Iguaçu. Resumos... Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2006. 1 CD-ROM. Área Microorganismos - pdf 898. Biblioteca(s): Embrapa Soja. |
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Registros recuperados : 13 | |
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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
10/10/2013 |
Data da última atualização: |
24/06/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
BEVITORI, R.; POPIELARSKA-KONIECZNA, M.; SANTOS, E. M. dos; GROSSI-DE-SÁ, M. F.; PETROFEZA, S. |
Afiliação: |
ROSANGELA BEVITORI, CNPAF; M. POPIELARSKA-KONIECZNA, JAGIELLONIAN UNIVERSITY; E. M. DOS SANTOS, UFG; MARIA FATIMA GROSSI DE SA, CENARGEN; S. PETROFEZA, UFG. |
Título: |
Morpho-anatomical characterization of mature embryo-derived callus of rice (Oryza sativa L.) suitable for transformation. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Protoplasma, New York, v. 251, n. 3, p. 545-554, May 2014. |
DOI: |
10.1007/s00709-013-0553-4 |
Idioma: |
Inglês |
Conteúdo: |
The objective of this study was to morphoanatomically characterize embryogenic rice calli during early induction of somatic embryogenesis of three Brazilian rice cultivars. Herein, we explored embryogenic units (EUs) from 2-week-old cut proliferated calli to verify whether they were suitable for Agrobacterium tumefasciens-mediated transformation. Histological analysis and scanning electron microscopy (SEM) were used to analyze these types of calli during early rice callogenesis in the cultivars BRS Primavera, BRS Bonança, and BRS Caiapó. The characteristics of the embryogenic cells were preserved in the EUs, which showed a globular, compact structure that contained tightly packed cells and thus rendered the cells suitable for transformation. The EUs of BRS Caiapó also maintained the characteristics of the nonembryogenic callus, such as an elongated morphology and a lack of cellular organization. In general, the observations of the histological sections corresponded with those of the SEM images. The histological analysis suggested that all cultivars used in these experiments have morphogenic potential. The EUs from proliferated 2-week-old cut calli maintained their embryogenic features. The EUs were subjected to Agrobacterium-mediated transformation, which exhibited a regeneration frequency of 58 % for transformed hygromycin-resistant cell lines. These results show that EUs from proliferated 2-week-old cut calli are suitable for plant transformation. |
Thesagro: |
Arroz; Embriogenese; Morfogenese; Oryza sativa. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179857/1/Bevitori2014-Article-Morpho-anatomicalCharacterizat.pdf
|
Marc: |
LEADER 02199naa a2200229 a 4500 001 1975223 005 2024-06-24 008 2014 bl uuuu u00u1 u #d 024 7 $a10.1007/s00709-013-0553-4$2DOI 100 1 $aBEVITORI, R. 245 $aMorpho-anatomical characterization of mature embryo-derived callus of rice (Oryza sativa L.) suitable for transformation.$h[electronic resource] 260 $c2014 520 $aThe objective of this study was to morphoanatomically characterize embryogenic rice calli during early induction of somatic embryogenesis of three Brazilian rice cultivars. Herein, we explored embryogenic units (EUs) from 2-week-old cut proliferated calli to verify whether they were suitable for Agrobacterium tumefasciens-mediated transformation. Histological analysis and scanning electron microscopy (SEM) were used to analyze these types of calli during early rice callogenesis in the cultivars BRS Primavera, BRS Bonança, and BRS Caiapó. The characteristics of the embryogenic cells were preserved in the EUs, which showed a globular, compact structure that contained tightly packed cells and thus rendered the cells suitable for transformation. The EUs of BRS Caiapó also maintained the characteristics of the nonembryogenic callus, such as an elongated morphology and a lack of cellular organization. In general, the observations of the histological sections corresponded with those of the SEM images. The histological analysis suggested that all cultivars used in these experiments have morphogenic potential. The EUs from proliferated 2-week-old cut calli maintained their embryogenic features. The EUs were subjected to Agrobacterium-mediated transformation, which exhibited a regeneration frequency of 58 % for transformed hygromycin-resistant cell lines. These results show that EUs from proliferated 2-week-old cut calli are suitable for plant transformation. 650 $aArroz 650 $aEmbriogenese 650 $aMorfogenese 650 $aOryza sativa 700 1 $aPOPIELARSKA-KONIECZNA, M. 700 1 $aSANTOS, E. M. dos 700 1 $aGROSSI-DE-SÁ, M. F. 700 1 $aPETROFEZA, S. 773 $tProtoplasma, New York$gv. 251, n. 3, p. 545-554, May 2014.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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