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| Registros recuperados : 13 | |
| 6. |  | BEVITORI, R.; POPIELARSKA-KONIECZNA, M.; SANTOS, E. M. dos; GROSSI-DE-SÁ, M. F.; PETROFEZA, S. Morpho-anatomical characterization of mature embryo-derived callus of rice (Oryza sativa L.) suitable for transformation. Protoplasma, New York, v. 251, n. 3, p. 545-554, May 2014.| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | OLIVEIRA, C. M.; FREITAS, M. A.; FALEIRO, V. de O.; PETROFEZA, S. Estratégia moleculares para identificação de Meloidogyne incognita, M. javanica, Pratylenchus brachyurus e P. coffese In: CONGRESSO BRASILEIRO DE NEMATOLOGIA, 31., 2013, Cuiabá. Anais... Cuiabá, MT: Sociedade Brasileira de Nematologia, 2013.| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 10. | | MAGALHÃES, L. F.; CUNHA, C. P. R.; LOBO JUNIOR, M.; PETROFEZA, S. Diversidade genética em populações de Sclerotinia sclerotiorum na cultura de feijoeiro (Phaseolus vulgaris L.). Tropical Plant Pathology, Brasília, DF, v. 36, p. 1124, ago. 2011. Suplemento, ref. 1576. Edição dos Resumos do 44 Congresso Brasileiro de Fitopatologia, Bento Gonçalves, ago. 2011. 1 CD-ROM.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 11. | | OLIVEIRA, M. B.; SANTOS, E. M. dos; INOCÊNCIO, A. P. M.; CUNHA, C. P. R.; LOBO JUNIOR, M.; PETROFEZA, S. Análise de enimas hidrolíticas durante a interação do fungo Sclerotinia sclerotiorum e plantas de feijoeiro (Phaseolus vulgaris L.). In: CONGRESSO DE GENÉTICA DO CENTRO-OESTE, 2., 2010, Anais... Goiânia. Goiânia: Universidade Federal de Goiás, 2010. 1 CD-ROM.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 12. | | BEVITORI, R.; OLIVEIRA, M. B.; GROSSI-DE-SÁ, M. F.; LANNA, A. C.; SILVEIRA, R. D. da; PETROFEZA, S. Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought. Genetics and Molecular Research, v. 13, n. 4, p. 9795-9805, 2014.| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | BARBOSA, S. R. C.; GOMES, E.; BRESEGHELO, L.; MOURA, A. L. D.; ALVES, E. B.; KNOB, A.; ALMEIDA, A. M. R.; LOBO JUNIOR, M.; PETROFEZA, S. Análise parcial do transcriptoma de Sclerotinia sclerotiorum durante a interação patogênica com feijão comum (Phaseolus vulgaris L.). In: CONGRESSO BRASILEIRO DE GENÉTICA, 52.; CONGRESO DE LA ASOCIACIÓN LATINOAMERICANA DE GENÉTICA, 12., 2006, Foz do Iguaçu. Resumos... Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2006. 1 CD-ROM. Área Microorganismos - pdf 898.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 13 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
16/06/2010 |
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Data da última atualização: |
02/09/2010 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
OLIVEIRA, M. B.; NASCIMENTO, L. B.; JUNIOR, M. L.; PETROFEZA, S. |
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Afiliação: |
M. B. OLIVEIRA, UNIVERSIDADE FEDERAL DE GOIÁS; L. B. NASCIMENTO, UNIVERSIDADE FEDERAL DE GOIÁS; MURILLO LOBO JUNIOR, CNPAF; S. PETROFEZA, UNIVERSIDADE FEDERAL DE GOIÁS. |
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Título: |
Characterization of the dry bean polygalacturonase-inhibiting protein (PGIP) gene family during Sclerotinia sclerotiorum (Sclerotiniaceae) infection. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 9, n. 2, p. 994-1004, 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Polygalacturonase-inhibiting proteins are leucine-rich repeat proteins that inhibit fungal endopolygalacturonases. The interaction of polygalacturonase-inhibiting protein with endopolygalacturonases limits the destructive potential of endopolygalacturonases and may trigger plant defense responses induced by oligogalacturonides. We examined the expression of fungal pg and plant Pvpgip genes in bean (Phaseolus vulgaris) stems infected with Sclerotinia sclerotiorum to determine whether any of them are associated with the infection process. Transcriptional analysis was carried out by means of semi-quantitative reverse transcription PCR or real-time PCR. The sspg1 gene was highly expressed during infection; sspg3 was regulated during the later phases of infection; sspg5 was more uniformly expressed during infection, whereas sspg6 was only weakly expressed. During the course of infection, Pvpgip1 transcripts were not detected at early stages, but they appeared 72 h post-inoculation. High levels of Pvpgip2 expression were observed during the initial phase of infection; the transcript peaked by 48 h post-inoculation and declined by 72 h post-inoculation. Pvpgip3 expression increased strongly at 96 h post-inoculation. Pvpgip4 was constantly present from 24 h post-inoculation until the end of the experiment. However, we detected higher levels of the Pvpgip4 transcript in the necrotic lesion area than in plants that had been mechanically wounded. Remarkably, only Pvpgip4 appeared to be moderately induced by mechanical wounding. These results provide evidence that endopolygalacturonases contribute to the infection process during host colonization by promoting the release of plant cell oligogalacturonides, which are powerful signaling molecules and may also activate plant defenses, such as polygalacturonase-inhibiting proteins. MenosPolygalacturonase-inhibiting proteins are leucine-rich repeat proteins that inhibit fungal endopolygalacturonases. The interaction of polygalacturonase-inhibiting protein with endopolygalacturonases limits the destructive potential of endopolygalacturonases and may trigger plant defense responses induced by oligogalacturonides. We examined the expression of fungal pg and plant Pvpgip genes in bean (Phaseolus vulgaris) stems infected with Sclerotinia sclerotiorum to determine whether any of them are associated with the infection process. Transcriptional analysis was carried out by means of semi-quantitative reverse transcription PCR or real-time PCR. The sspg1 gene was highly expressed during infection; sspg3 was regulated during the later phases of infection; sspg5 was more uniformly expressed during infection, whereas sspg6 was only weakly expressed. During the course of infection, Pvpgip1 transcripts were not detected at early stages, but they appeared 72 h post-inoculation. High levels of Pvpgip2 expression were observed during the initial phase of infection; the transcript peaked by 48 h post-inoculation and declined by 72 h post-inoculation. Pvpgip3 expression increased strongly at 96 h post-inoculation. Pvpgip4 was constantly present from 24 h post-inoculation until the end of the experiment. However, we detected higher levels of the Pvpgip4 transcript in the necrotic lesion area than in plants that had been mechanically wounded. Remarkably, only Pvpgip4 appeared to be... Mostrar Tudo |
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Palavras-Chave: |
Endopolygalacturonase; Polygalacturonase-inhibiting protein. |
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Thesagro: |
Doença fúngica; Feijão; Mofo branco; Phaseolus vulgaris; Sclerotinia sclerotiorum. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/18863/1/gmr776.pdf
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Marc: |
LEADER 02653naa a2200241 a 4500
001 1855158
005 2010-09-02
008 2010 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, M. B.
245 $aCharacterization of the dry bean polygalacturonase-inhibiting protein (PGIP) gene family during Sclerotinia sclerotiorum (Sclerotiniaceae) infection.$h[electronic resource]
260 $c2010
520 $aPolygalacturonase-inhibiting proteins are leucine-rich repeat proteins that inhibit fungal endopolygalacturonases. The interaction of polygalacturonase-inhibiting protein with endopolygalacturonases limits the destructive potential of endopolygalacturonases and may trigger plant defense responses induced by oligogalacturonides. We examined the expression of fungal pg and plant Pvpgip genes in bean (Phaseolus vulgaris) stems infected with Sclerotinia sclerotiorum to determine whether any of them are associated with the infection process. Transcriptional analysis was carried out by means of semi-quantitative reverse transcription PCR or real-time PCR. The sspg1 gene was highly expressed during infection; sspg3 was regulated during the later phases of infection; sspg5 was more uniformly expressed during infection, whereas sspg6 was only weakly expressed. During the course of infection, Pvpgip1 transcripts were not detected at early stages, but they appeared 72 h post-inoculation. High levels of Pvpgip2 expression were observed during the initial phase of infection; the transcript peaked by 48 h post-inoculation and declined by 72 h post-inoculation. Pvpgip3 expression increased strongly at 96 h post-inoculation. Pvpgip4 was constantly present from 24 h post-inoculation until the end of the experiment. However, we detected higher levels of the Pvpgip4 transcript in the necrotic lesion area than in plants that had been mechanically wounded. Remarkably, only Pvpgip4 appeared to be moderately induced by mechanical wounding. These results provide evidence that endopolygalacturonases contribute to the infection process during host colonization by promoting the release of plant cell oligogalacturonides, which are powerful signaling molecules and may also activate plant defenses, such as polygalacturonase-inhibiting proteins.
650 $aDoença fúngica
650 $aFeijão
650 $aMofo branco
650 $aPhaseolus vulgaris
650 $aSclerotinia sclerotiorum
653 $aEndopolygalacturonase
653 $aPolygalacturonase-inhibiting protein
700 1 $aNASCIMENTO, L. B.
700 1 $aJUNIOR, M. L.
700 1 $aPETROFEZA, S.
773 $tGenetics and Molecular Research$gv. 9, n. 2, p. 994-1004, 2010.
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Embrapa Arroz e Feijão (CNPAF) |
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