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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
18/01/2013 |
Data da última atualização: |
16/02/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SUDANO, M. J.; SANTOS, V. G.; TATA, A.; FERREIRA, C. R.; PASCHOAL, D. M.; MACHADO, R.; BURATINI, J.; EBERLIN, M. N.; LANDIM-ALVARENGA, F. D. C. |
Afiliação: |
MATEUS J. SUDANO, UNESP; VANESSA G. SANTOS, University of Campinas; ALESSANDRA TATA, University of Campinas; CHRISTINA R. FERREIRA, University of Campinas; DANIELA M. PASCHOAL, UNESP; RUI MACHADO, CPPSE; JOSÉ BURATINI, UNESP; MARCOS N. EBERLIN, University of Campinas; FERNANDA D.C. LANDIM-ALVARENGA, UNESP. |
Título: |
Phosphatidylcholine and sphingomyelin profiles vary in Bos taurus indicus and Bos taurus taurus in vitro- and in vivo-produced blastocysts. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Biology of Reproduction, v. 87, n. 6, p. 1-11, 2012. |
DOI: |
10.1095/biolreprod.112.102897 |
Idioma: |
Inglês |
Conteúdo: |
Lipid droplets, subspecies (Bos taurus indicus vs. Bos taurus taurus), and in vitro culture are known to influence cryopreservation of bovine embryos. Limited information is available regarding differences in membrane lipids in embryo, such as phosphatidylcholines (PC) and sphingomyelins (SM). The objective of the present study was to compare the profiles of several PC and SM species and relate this information to cytoplasmic lipid levels present in Nellore (B. taurus indicus) and Simmental (B. taurus taurus) blastocysts produced in vitro (IVP) or in vivo (ET). Simmental and IVP embryos had more cytoplasmic lipid content than Nellore and ET embryos (n ¼ 30). Blastocysts were submitted to matrix-assisted laser desorption/ionization mass spectrometry. Differences in the PC profile were addressed by principal component analysis. The lipid species with PC (32:1) and PC (34:1) had higher ion abundances in Nellore embryos, whereas PC (34:2) was higher in Simmental embryos. IVP embryos had less abundant ions of PC (32:1), PC (34:2), and PC (36:5) compared to ET embryos. Moreover, ion abundance of PC (32:0) was higher in both Nellore and Simmental IVP embryos compared to ET embryos. Therefore, mass spectrometry profiles of PC and SM species significantly differ with regard to unsaturation level and carbon chain composition in bovine blastocysts due to subspecies and in vitro culture conditions. Because PC abundances of Nellore and Simmental embryos were distinct (34:1 vs. 34:2), as were those of IVP and ET embryos (32:0 vs. 36:5), they are potential markers of postcryopreservation embryonic survival. MenosLipid droplets, subspecies (Bos taurus indicus vs. Bos taurus taurus), and in vitro culture are known to influence cryopreservation of bovine embryos. Limited information is available regarding differences in membrane lipids in embryo, such as phosphatidylcholines (PC) and sphingomyelins (SM). The objective of the present study was to compare the profiles of several PC and SM species and relate this information to cytoplasmic lipid levels present in Nellore (B. taurus indicus) and Simmental (B. taurus taurus) blastocysts produced in vitro (IVP) or in vivo (ET). Simmental and IVP embryos had more cytoplasmic lipid content than Nellore and ET embryos (n ¼ 30). Blastocysts were submitted to matrix-assisted laser desorption/ionization mass spectrometry. Differences in the PC profile were addressed by principal component analysis. The lipid species with PC (32:1) and PC (34:1) had higher ion abundances in Nellore embryos, whereas PC (34:2) was higher in Simmental embryos. IVP embryos had less abundant ions of PC (32:1), PC (34:2), and PC (36:5) compared to ET embryos. Moreover, ion abundance of PC (32:0) was higher in both Nellore and Simmental IVP embryos compared to ET embryos. Therefore, mass spectrometry profiles of PC and SM species significantly differ with regard to unsaturation level and carbon chain composition in bovine blastocysts due to subspecies and in vitro culture conditions. Because PC abundances of Nellore and Simmental embryos were distinct (34:1 vs. 34:2), as ... Mostrar Tudo |
Palavras-Chave: |
Cryotolerance biomarkers; Lipidomics; MALDI MS; Zebuine. |
Thesaurus Nal: |
cryopreservation; in vitro culture; mass spectrometry; taurine. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02600naa a2200325 a 4500 001 1945818 005 2023-02-16 008 2012 bl uuuu u00u1 u #d 024 7 $a10.1095/biolreprod.112.102897$2DOI 100 1 $aSUDANO, M. J. 245 $aPhosphatidylcholine and sphingomyelin profiles vary in Bos taurus indicus and Bos taurus taurus in vitro- and in vivo-produced blastocysts.$h[electronic resource] 260 $c2012 520 $aLipid droplets, subspecies (Bos taurus indicus vs. Bos taurus taurus), and in vitro culture are known to influence cryopreservation of bovine embryos. Limited information is available regarding differences in membrane lipids in embryo, such as phosphatidylcholines (PC) and sphingomyelins (SM). The objective of the present study was to compare the profiles of several PC and SM species and relate this information to cytoplasmic lipid levels present in Nellore (B. taurus indicus) and Simmental (B. taurus taurus) blastocysts produced in vitro (IVP) or in vivo (ET). Simmental and IVP embryos had more cytoplasmic lipid content than Nellore and ET embryos (n ¼ 30). Blastocysts were submitted to matrix-assisted laser desorption/ionization mass spectrometry. Differences in the PC profile were addressed by principal component analysis. The lipid species with PC (32:1) and PC (34:1) had higher ion abundances in Nellore embryos, whereas PC (34:2) was higher in Simmental embryos. IVP embryos had less abundant ions of PC (32:1), PC (34:2), and PC (36:5) compared to ET embryos. Moreover, ion abundance of PC (32:0) was higher in both Nellore and Simmental IVP embryos compared to ET embryos. Therefore, mass spectrometry profiles of PC and SM species significantly differ with regard to unsaturation level and carbon chain composition in bovine blastocysts due to subspecies and in vitro culture conditions. Because PC abundances of Nellore and Simmental embryos were distinct (34:1 vs. 34:2), as were those of IVP and ET embryos (32:0 vs. 36:5), they are potential markers of postcryopreservation embryonic survival. 650 $acryopreservation 650 $ain vitro culture 650 $amass spectrometry 650 $ataurine 653 $aCryotolerance biomarkers 653 $aLipidomics 653 $aMALDI MS 653 $aZebuine 700 1 $aSANTOS, V. G. 700 1 $aTATA, A. 700 1 $aFERREIRA, C. R. 700 1 $aPASCHOAL, D. M. 700 1 $aMACHADO, R. 700 1 $aBURATINI, J. 700 1 $aEBERLIN, M. N. 700 1 $aLANDIM-ALVARENGA, F. D. C. 773 $tBiology of Reproduction$gv. 87, n. 6, p. 1-11, 2012.
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| Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
07/12/2015 |
Data da última atualização: |
20/06/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
PETRIZ, B. A.; ALMEIDA, J. A.; GOMES, C. P. C.; PEREIRA, R. W.; MURAD, A. M.; FRANCO, O. L. |
Afiliação: |
Bernardo A. Petriz, UCB; Jeeser A. Almeida, UCB; Clarissa P. C. Gomes, UCB; Rinaldo W. Pereira, UCB; ANDRE MELRO MURAD, CENARGEN; Octavio l. Franco, UCB. |
Título: |
NanoUPLC/MSE proteomic analysis reveals modulation on left ventricle proteome from hypertensive rats after exercise training. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Journal of Proteomics, v. 113, p. 351-365, 2015. |
Idioma: |
Inglês |
Conteúdo: |
NanoUPLC/MSE was used to verify the effects of 8 weeks of low (SHR-LIT = 4) and high (SHR-HIT = 4) intensity training over the left ventricle proteome of hypertensive rats (SHR-C = 4). Training enhanced the aerobic capacity and reduced the systolic blood pressure in all exercised rats.NanoUPLC/MSE identified 250 proteins, with 233 in common to all groups and 16 exclusive to SHR-C, 2 to SHR-LIT, and 2 to the SHR-HIT. Cardiac hypertrophy related proteins appeared only in SHR-C. The SHR-LIT enhanced the abundance of 30 proteins and diminished 6, while SHR-HIT enhanced the abundance of 39 proteins and reduced other 7. The levels of metabolic (? and ?-enolase, adenine phosphoribosultransferase, and cytochrome b-c1), myofibril (myosin light chain 4, tropomyosin ? and ?-chain), and transporter proteins (hemoglobin, serum albumin, and hemopexin)were increased by both intensities. Transcription regulator and histone variants were enhanced by SHR-LIT and SHR-HIT respectively. SHR-LIT reduced the concentration of myosin binding protein C, while desmin and membrane voltage dependent anion selective channel protein-3 were reduced only by SHR-HIT. In addition, polyubiquitin B and C, and transcription regulators decreased in both intensities. Exercise also increased the concentration of anti-oxidant proteins, peroxiredozin-6 and glutathione peroxidase-1. Biological significance Pathologic left ventricle hypertrophy if one of the major outcomes of hypertension being a strong predictor of heart failure. Among the various risk factors for cardiovascular disorders, arterial hypertension is responsible for the highest rates of mortality worldwide. In this way,this present study contribute to the understanding of the molecular mechanisms involved in the attenuation of hypertension and the regression of pathological cardiac hypertrophy induced by exercise training. MenosNanoUPLC/MSE was used to verify the effects of 8 weeks of low (SHR-LIT = 4) and high (SHR-HIT = 4) intensity training over the left ventricle proteome of hypertensive rats (SHR-C = 4). Training enhanced the aerobic capacity and reduced the systolic blood pressure in all exercised rats.NanoUPLC/MSE identified 250 proteins, with 233 in common to all groups and 16 exclusive to SHR-C, 2 to SHR-LIT, and 2 to the SHR-HIT. Cardiac hypertrophy related proteins appeared only in SHR-C. The SHR-LIT enhanced the abundance of 30 proteins and diminished 6, while SHR-HIT enhanced the abundance of 39 proteins and reduced other 7. The levels of metabolic (? and ?-enolase, adenine phosphoribosultransferase, and cytochrome b-c1), myofibril (myosin light chain 4, tropomyosin ? and ?-chain), and transporter proteins (hemoglobin, serum albumin, and hemopexin)were increased by both intensities. Transcription regulator and histone variants were enhanced by SHR-LIT and SHR-HIT respectively. SHR-LIT reduced the concentration of myosin binding protein C, while desmin and membrane voltage dependent anion selective channel protein-3 were reduced only by SHR-HIT. In addition, polyubiquitin B and C, and transcription regulators decreased in both intensities. Exercise also increased the concentration of anti-oxidant proteins, peroxiredozin-6 and glutathione peroxidase-1. Biological significance Pathologic left ventricle hypertrophy if one of the major outcomes of hypertension being a strong predictor of he... Mostrar Tudo |
Palavras-Chave: |
Análise proteômica; Hipertensão. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02514naa a2200205 a 4500 001 2030868 005 2024-06-20 008 2015 bl uuuu u00u1 u #d 100 1 $aPETRIZ, B. A. 245 $aNanoUPLC/MSE proteomic analysis reveals modulation on left ventricle proteome from hypertensive rats after exercise training.$h[electronic resource] 260 $c2015 520 $aNanoUPLC/MSE was used to verify the effects of 8 weeks of low (SHR-LIT = 4) and high (SHR-HIT = 4) intensity training over the left ventricle proteome of hypertensive rats (SHR-C = 4). Training enhanced the aerobic capacity and reduced the systolic blood pressure in all exercised rats.NanoUPLC/MSE identified 250 proteins, with 233 in common to all groups and 16 exclusive to SHR-C, 2 to SHR-LIT, and 2 to the SHR-HIT. Cardiac hypertrophy related proteins appeared only in SHR-C. The SHR-LIT enhanced the abundance of 30 proteins and diminished 6, while SHR-HIT enhanced the abundance of 39 proteins and reduced other 7. The levels of metabolic (? and ?-enolase, adenine phosphoribosultransferase, and cytochrome b-c1), myofibril (myosin light chain 4, tropomyosin ? and ?-chain), and transporter proteins (hemoglobin, serum albumin, and hemopexin)were increased by both intensities. Transcription regulator and histone variants were enhanced by SHR-LIT and SHR-HIT respectively. SHR-LIT reduced the concentration of myosin binding protein C, while desmin and membrane voltage dependent anion selective channel protein-3 were reduced only by SHR-HIT. In addition, polyubiquitin B and C, and transcription regulators decreased in both intensities. Exercise also increased the concentration of anti-oxidant proteins, peroxiredozin-6 and glutathione peroxidase-1. Biological significance Pathologic left ventricle hypertrophy if one of the major outcomes of hypertension being a strong predictor of heart failure. Among the various risk factors for cardiovascular disorders, arterial hypertension is responsible for the highest rates of mortality worldwide. In this way,this present study contribute to the understanding of the molecular mechanisms involved in the attenuation of hypertension and the regression of pathological cardiac hypertrophy induced by exercise training. 653 $aAnálise proteômica 653 $aHipertensão 700 1 $aALMEIDA, J. A. 700 1 $aGOMES, C. P. C. 700 1 $aPEREIRA, R. W. 700 1 $aMURAD, A. M. 700 1 $aFRANCO, O. L. 773 $tJournal of Proteomics$gv. 113, p. 351-365, 2015.
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