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| Registros recuperados : 40 | |
| 30. |  | AVILA, A. C. de; HAAN, P. de; KITAJIMA, E. W.; RESENDE, R. de O.; GOLDBACH, R. W.; PETERS, D. Characterization of a distinct isolate of tomato spotted wilt virus (TSWV) from impatiens in the Netherlands. Fitopatologia Brasileira, Brasilia, v.16, n.2, p.25, jun. 1991. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 31. | | POZZER, L.; BEZERRA, I. C.; KORMELINK, R.; PRINS, M.; PETERS, D.; RESENDE, R. de O.; AVILA, A. C. de. Characterization of a tospovirus isolate of iris yellow spot virus associated with a disease in onion fields in Brazil. Plant Diseases, v.83, n.4, p.345-350, Apr. 1999.| Biblioteca(s): Embrapa Hortaliças. |
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| 32. | | HAAN, P. de; AVILA, A. C. de; KORMELINK, R.; WESTERBROEK, A.; GIELEN, J. J. I.; PETERS, D.; GOLBACH, R. The nucleotide sequence of the S RNA of impotiens necrotic spot virus, a novel tospovirus. Federation of European Biochemical Societies, v.306, n.1, p.27-32, 1992.| Biblioteca(s): Embrapa Semiárido. |
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| 34. | | RESENDE, R. de O.; HAAN, P. de; AVILA, A. C. de; KITAJIMA, E. W.; KORMELINK, R.; GOLDBACH, R.; PETERS, D. Generation of envelope and defective interfering RNA mutants of tomato spotted wilt virus by machanical passage. Journal of General Virology, v. 72, p.2335-2383, 1991.| Biblioteca(s): Embrapa Hortaliças. |
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| 35. | | INOUE-NAGATA, A. K.; KOMELINK, R.; SGRO, J. Y.; NAGATA, T.; KITAJIMA, E. W.; GOLDBACH, E. W.; PETERS, D. Tomato spotted wilt virus defective interfering RNAs, important features of their generation and survival. In:INTERNATIONAL SYMPOSIUM ON TOSPOVIRUSES AND THRIPS IN FLORAL AND VEGETAVBLE CROPS, 4., 1998, Wageningen. Recent progress in tospovirus thrips research: abstracts of papers and poster presentations. Wageningen: Experimental Plant Science / Production Ecology, 1998. p. 4-5.| Biblioteca(s): Embrapa Hortaliças. |
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| 36. | | AVILA, A. C. de; HAAN, de P.; KITAJIMA, E. W.; KORMELINK, R.; RESENDE R. de O.; GOLDBACH, R. W.; PETERS, D. Characterization of a distinct isolate of tomato spotted wilt virus (TSWV) from impatiens sp. in the Netherlands. Phytopathology, v.134, n.2, p.133-151, 1992.| Biblioteca(s): Embrapa Hortaliças. |
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| 37. | | AVILA, A. C. de; PENA, L.; KITAJIMA, E. W.; RESENDE, R. de O.; DIAS-MUGICA, M. V.; DIAZ-RUIZ, J. R.; PETERS, D. Characterization of tomato spotted wilt virus (TSWV), isolated from Capsicum annuum L. in the Canary Islands. Phytopathologia Mediterranea, v. 30, p. 23-28, 1991.| Biblioteca(s): Embrapa Hortaliças. |
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| 38. | | AVILA, A. C. de; POZZER, L.; BEZERRA, I.; KORMELINK, R.; PRINS, M.; PETERS, D.; NAGATA, T.; KITAJIMA, E. W.; RESENDE, R. de O. Diversity of tospoviruses in Brazil. In: INTERNATIONAL SYMPOSIUM ON TOSPOVIRUSES AND THRIPS IN FLORAL AND VEGETABLE CROPS, 4., 1998, Wageningen, NL. Recent progress in tospovirus and thrips research: abstracts of papers and poster presentations. Wageningen: Experimental Plant Sciences / Production Ecology, 1998. p.32-34.| Biblioteca(s): Embrapa Hortaliças. |
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| 39. | | AVILA, A. C. de; HAAN, P. de; SMEETS, M. L. L.; RESENDE, R. de O.; KORMELINK, R.; KITAJIMA, E. W.; GOLDBACH, R. W.; PETERS, D. Distinct levels of relationship between tospovirus isolates. Archives of Virology, v.128, p.211-227, 1993.| Biblioteca(s): Embrapa Hortaliças. |
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| 40. | | AVILA, A. C. de; HAAN, P. de; KORMELINK, R.; RESENDE, R. de O.; POELWIJK, F. van; WIJKAMP, I.; GOLDBACH, R. W.; PETERS, D. Tomato spotted wilt virus: genne structure, classification, detection and genetically engineered resistance. In: RISHI, N.; AHUJA, K.L.; SINGH, B.P., (Ed.). Virology in the tropics. New Delhi: Malhotra Publishing House, 1994. p.95-105.| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 40 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Hortaliças. |
|
Data corrente: |
31/03/1993 |
|
Data da última atualização: |
05/03/2024 |
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Autoria: |
RESENDE, R. de O.; HAAN, P. de; VOSSEN, E. van de; AVILA, A. C. de; GOLDBACH, R.; PETERS, D. |
|
Afiliação: |
RENATO DE OLIVEIRA RESENDE; PETER DE HAAN; EDWIN VAN DE VOSSEN; ANTONIO CARLOS DE AVILA, CNPH; ROB GOLDBACH; DICK PETERS. |
|
Título: |
Defective interfering L RNA segments of tomato spotted wilt virus retain both virus genome termini and have extensive internal deletions. |
|
Ano de publicação: |
1992 |
|
Fonte/Imprenta: |
Journal of General Virology, v. 73, p. 2509-2516, 1992. |
|
DOI: |
https://doi.org/10.1099/0022-1317-73-10-2509 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Defective interfering (DI) RNA molecules derived from the genomic L RNA segment of tomato spotted wilt virus (TSWV) were generated during sequential passage of the virus at high multiplicity. Characterization of DI RNAs from four distinct isolates by Northern blot analysis and sequence determination revealed that both the 5′ and 3′ genomic termini were retained in these molecules. Each DI RNA contained a single internal deletion of approximately 60% to 80% of the L RNA segment. All DI RNAs studied maintain an open reading frame (ORF) which suggests that these defective molecules should be translatable by ribosomes. Detection of only defective molecules with ORFs indicates either that association with ribosomes or translation is a prerequisite for the selection and maintenance of replicating DI RNAs, or that the truncated proteins produced play a role in their selection or replication. Analysis of the junction sites in the DI RNAs showed that short nucleotide sequences are repeated, one at the release and another at the reinitiation point on the L RNA. One of these is lost during the generation of the DI molecules. The presence of repeated sequences at the junction sites seems to be unique for tospovirus DI L RNAs; they have not been described for other DI systems of either positive- or negative-strand RNA viruses. A model for TSWV DI RNA generation is proposed in which the viral polymerase can ‘jump’ across the internal sequences from one secondary structure to another containing the repeated sequences, during the replication of the viral complementary L RNA segment. MenosDefective interfering (DI) RNA molecules derived from the genomic L RNA segment of tomato spotted wilt virus (TSWV) were generated during sequential passage of the virus at high multiplicity. Characterization of DI RNAs from four distinct isolates by Northern blot analysis and sequence determination revealed that both the 5′ and 3′ genomic termini were retained in these molecules. Each DI RNA contained a single internal deletion of approximately 60% to 80% of the L RNA segment. All DI RNAs studied maintain an open reading frame (ORF) which suggests that these defective molecules should be translatable by ribosomes. Detection of only defective molecules with ORFs indicates either that association with ribosomes or translation is a prerequisite for the selection and maintenance of replicating DI RNAs, or that the truncated proteins produced play a role in their selection or replication. Analysis of the junction sites in the DI RNAs showed that short nucleotide sequences are repeated, one at the release and another at the reinitiation point on the L RNA. One of these is lost during the generation of the DI molecules. The presence of repeated sequences at the junction sites seems to be unique for tospovirus DI L RNAs; they have not been described for other DI systems of either positive- or negative-strand RNA viruses. A model for TSWV DI RNA generation is proposed in which the viral polymerase can ‘jump’ across the internal sequences from one secondary structure to another conta... Mostrar Tudo |
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Palavras-Chave: |
Caracterizacao; Characterization; DI L RNA; Generation; Geracao; Maintenance; TSWV; Vira-Cabeca. |
|
Thesagro: |
Manutenção. |
|
Thesaurus NAL: |
Tomato spotted wilt virus. |
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Categoria do assunto: |
-- |
|
Marc: |
LEADER 02548naa a2200313 a 4500
001 1749565
005 2024-03-05
008 1992 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1099/0022-1317-73-10-2509$2DOI
100 1 $aRESENDE, R. de O.
245 $aDefective interfering L RNA segments of tomato spotted wilt virus retain both virus genome termini and have extensive internal deletions.$h[electronic resource]
260 $c1992
520 $aDefective interfering (DI) RNA molecules derived from the genomic L RNA segment of tomato spotted wilt virus (TSWV) were generated during sequential passage of the virus at high multiplicity. Characterization of DI RNAs from four distinct isolates by Northern blot analysis and sequence determination revealed that both the 5′ and 3′ genomic termini were retained in these molecules. Each DI RNA contained a single internal deletion of approximately 60% to 80% of the L RNA segment. All DI RNAs studied maintain an open reading frame (ORF) which suggests that these defective molecules should be translatable by ribosomes. Detection of only defective molecules with ORFs indicates either that association with ribosomes or translation is a prerequisite for the selection and maintenance of replicating DI RNAs, or that the truncated proteins produced play a role in their selection or replication. Analysis of the junction sites in the DI RNAs showed that short nucleotide sequences are repeated, one at the release and another at the reinitiation point on the L RNA. One of these is lost during the generation of the DI molecules. The presence of repeated sequences at the junction sites seems to be unique for tospovirus DI L RNAs; they have not been described for other DI systems of either positive- or negative-strand RNA viruses. A model for TSWV DI RNA generation is proposed in which the viral polymerase can ‘jump’ across the internal sequences from one secondary structure to another containing the repeated sequences, during the replication of the viral complementary L RNA segment.
650 $aTomato spotted wilt virus
650 $aManutenção
653 $aCaracterizacao
653 $aCharacterization
653 $aDI L RNA
653 $aGeneration
653 $aGeracao
653 $aMaintenance
653 $aTSWV
653 $aVira-Cabeca
700 1 $aHAAN, P. de
700 1 $aVOSSEN, E. van de
700 1 $aAVILA, A. C. de
700 1 $aGOLDBACH, R.
700 1 $aPETERS, D.
773 $tJournal of General Virology$gv. 73, p. 2509-2516, 1992.
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