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| Registros recuperados : 172 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
13/05/2016 |
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Data da última atualização: |
11/10/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SCHWAB, S.; PESSOA, C. A.; BERGAMI, A. A. de L.; FIGUEIREDO, N. L. A.; TEIXEIRA, K. R. dos S.; BALDANI, J. I. |
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Afiliação: |
STEFAN SCHWAB, CNPAB; CRISTINA ALVES PESSOA, UFRJ; AMANDA APARECIDADE DE LIMA BERGAMI, UFRRJ; NATHÁLIA LIMA AZEVEDO FIGUEIREDO, UFRRJ; KATIA REGINA DOS SANTOS TEIXEIRA, CNPAB; JOSE IVO BALDANI, CNPAB. |
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Título: |
Isolation and characterization of active promoters from Gluconacetobacter diazotrophicus strain PAL5 using a promoter-trapping plasmid |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Archives of Microbiology, v. 198, n. 5, p. 445?458, 2016. |
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ISSN: |
1432-072X |
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DOI: |
10.1007/s00203-016-1203-y |
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Idioma: |
Inglês |
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Conteúdo: |
Gluconacetobacter diazotrophicus is a nitrogen-fixing, endophytic bacterium that has the potential to promote plant growth and increase yield. Genetically modified strains might get more benefits to host plants, including through expression of useful proteins, such as Cry toxins from B. thuringiensis, or enzymes involved in phytohormone production, proteins with antagonistic activity for phytopathogens, or that improve nutrient utilization by the plant. For that, expression systems for G. diazotrophicus are needed, which requires active promoters fused to foreign (or innate) genes. This article describes the construction of a G. diazotrophicus PAL5 promoter library using a promoter-less lacZ-bearing vector, and the identification of six active promoters through ?-galactosidase activity assays, sequencing and localization in the bacterial genome. The characterized promoters, which are located on distinct regions of the bacterial genome and encoding either sense or antisense transcripts, present variable expression strengths and might be used in the future for expressing useful proteins. |
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Palavras-Chave: |
Endophytic diazotroph; Plant growth promotion; Promoter trap; Protein expression system. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
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001 2044977
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008 2016 bl uuuu u00u1 u #d
022 $a1432-072X
024 7 $a10.1007/s00203-016-1203-y$2DOI
100 1 $aSCHWAB, S.
245 $aIsolation and characterization of active promoters from Gluconacetobacter diazotrophicus strain PAL5 using a promoter-trapping plasmid
260 $c2016
520 $aGluconacetobacter diazotrophicus is a nitrogen-fixing, endophytic bacterium that has the potential to promote plant growth and increase yield. Genetically modified strains might get more benefits to host plants, including through expression of useful proteins, such as Cry toxins from B. thuringiensis, or enzymes involved in phytohormone production, proteins with antagonistic activity for phytopathogens, or that improve nutrient utilization by the plant. For that, expression systems for G. diazotrophicus are needed, which requires active promoters fused to foreign (or innate) genes. This article describes the construction of a G. diazotrophicus PAL5 promoter library using a promoter-less lacZ-bearing vector, and the identification of six active promoters through ?-galactosidase activity assays, sequencing and localization in the bacterial genome. The characterized promoters, which are located on distinct regions of the bacterial genome and encoding either sense or antisense transcripts, present variable expression strengths and might be used in the future for expressing useful proteins.
653 $aEndophytic diazotroph
653 $aPlant growth promotion
653 $aPromoter trap
653 $aProtein expression system
700 1 $aPESSOA, C. A.
700 1 $aBERGAMI, A. A. de L.
700 1 $aFIGUEIREDO, N. L. A.
700 1 $aTEIXEIRA, K. R. dos S.
700 1 $aBALDANI, J. I.
773 $tArchives of Microbiology$gv. 198, n. 5, p. 445?458, 2016.
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