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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
29/03/2016 |
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Data da última atualização: |
28/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AGUIRRE, A. de A. R.; LOBO, F. P.; ANDREOTTI, R. |
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Afiliação: |
ANDRÉ DE ABREU RANGEL AGUIRRE, FAMEZ/UFMS; FRANCISCO PEREIRA LOBO, CNPTIA; RENATO ANDREOTTI E SILVA, CNPGC. |
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Título: |
Design of the ATAQ peptide and its evaluation as an immunogen to develop a Rhipicephalus vaccine. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 221, p. 30-38, 2016 |
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Idioma: |
Inglês |
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Conteúdo: |
Tick infestation may cause several problems including affecting domestic animal health and reducing the production of meat and milk, among others. Resistance to several classes of acaricides have been reported, forcing researchers to search for alternative measures, such as vaccines against ticks, to ensure tick control while having no or at least low negative impacts on the environment and public health. However, the current commercially available vaccines in different strains of Rhipicephalus microplus are reported to be of low efficacy. Fortunately, reverse vaccinology approaches have shown positive results in the new generation of vaccines. On this basis, a synthetic peptide from the ATAQ protein, which is present in the gut and Malpighi tubes of R. microplus, was synthesized. The ATAQ proteins were isolated, characterized and sequenced from several species of the genus Rhipicephalus. The alignment showed 93.3% identity among DNA sequences of ATAQs from these species. Because of this, immunization trials with this peptide were conducted on mice, rabbits and cattle to evaluate the humoral immune response and the efficacy against Rhipicephalus sanguineus in addition to R. microplus. Based on recent results, we conclude that reverse vaccinology is a promising approach because it is more accurate and faster than conventional methods in the detection of potential antigens to use in anti-tick vaccines. It is not only applicable against R. microplus but also against tick species that play important roles in spreading other diseases. ATAQ proteins should be considered as the antigen in new trials to develop a multi-antigenic vaccine. Although these peptides behave as hapten and are not able to be recognized by the immune system on its own, using carriers and adjuvants helps its presentation and induces strong immune responses. Furthermore, an efficiency of 35% reduction in overall life cycle parameters was reported for R. microplus (98% for ELISA responder animals) and 47% for R. sanguineus. Although not yet enough to prevent the environment to infestation of ticks, this still constitutes a promising strategy that could be applied to integrated measures on tick control and in new research that develops anti-tick vaccines. MenosTick infestation may cause several problems including affecting domestic animal health and reducing the production of meat and milk, among others. Resistance to several classes of acaricides have been reported, forcing researchers to search for alternative measures, such as vaccines against ticks, to ensure tick control while having no or at least low negative impacts on the environment and public health. However, the current commercially available vaccines in different strains of Rhipicephalus microplus are reported to be of low efficacy. Fortunately, reverse vaccinology approaches have shown positive results in the new generation of vaccines. On this basis, a synthetic peptide from the ATAQ protein, which is present in the gut and Malpighi tubes of R. microplus, was synthesized. The ATAQ proteins were isolated, characterized and sequenced from several species of the genus Rhipicephalus. The alignment showed 93.3% identity among DNA sequences of ATAQs from these species. Because of this, immunization trials with this peptide were conducted on mice, rabbits and cattle to evaluate the humoral immune response and the efficacy against Rhipicephalus sanguineus in addition to R. microplus. Based on recent results, we conclude that reverse vaccinology is a promising approach because it is more accurate and faster than conventional methods in the detection of potential antigens to use in anti-tick vaccines. It is not only applicable against R. microplus but also against tick specie... Mostrar Tudo |
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Palavras-Chave: |
ATAQ; Reverse vaccinology; Synthetic peptide. |
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Thesaurus Nal: |
Immunization; Rhipicephalus microplus; Rhipicephalus sanguineus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/158239/1/Design-of-the-ATAQ-peptide-and-its-evaluation.pdf
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Marc: |
LEADER 02914naa a2200217 a 4500
001 2041997
005 2017-03-28
008 2016 bl uuuu u00u1 u #d
100 1 $aAGUIRRE, A. de A. R.
245 $aDesign of the ATAQ peptide and its evaluation as an immunogen to develop a Rhipicephalus vaccine.$h[electronic resource]
260 $c2016
520 $aTick infestation may cause several problems including affecting domestic animal health and reducing the production of meat and milk, among others. Resistance to several classes of acaricides have been reported, forcing researchers to search for alternative measures, such as vaccines against ticks, to ensure tick control while having no or at least low negative impacts on the environment and public health. However, the current commercially available vaccines in different strains of Rhipicephalus microplus are reported to be of low efficacy. Fortunately, reverse vaccinology approaches have shown positive results in the new generation of vaccines. On this basis, a synthetic peptide from the ATAQ protein, which is present in the gut and Malpighi tubes of R. microplus, was synthesized. The ATAQ proteins were isolated, characterized and sequenced from several species of the genus Rhipicephalus. The alignment showed 93.3% identity among DNA sequences of ATAQs from these species. Because of this, immunization trials with this peptide were conducted on mice, rabbits and cattle to evaluate the humoral immune response and the efficacy against Rhipicephalus sanguineus in addition to R. microplus. Based on recent results, we conclude that reverse vaccinology is a promising approach because it is more accurate and faster than conventional methods in the detection of potential antigens to use in anti-tick vaccines. It is not only applicable against R. microplus but also against tick species that play important roles in spreading other diseases. ATAQ proteins should be considered as the antigen in new trials to develop a multi-antigenic vaccine. Although these peptides behave as hapten and are not able to be recognized by the immune system on its own, using carriers and adjuvants helps its presentation and induces strong immune responses. Furthermore, an efficiency of 35% reduction in overall life cycle parameters was reported for R. microplus (98% for ELISA responder animals) and 47% for R. sanguineus. Although not yet enough to prevent the environment to infestation of ticks, this still constitutes a promising strategy that could be applied to integrated measures on tick control and in new research that develops anti-tick vaccines.
650 $aImmunization
650 $aRhipicephalus microplus
650 $aRhipicephalus sanguineus
653 $aATAQ
653 $aReverse vaccinology
653 $aSynthetic peptide
700 1 $aLOBO, F. P.
700 1 $aANDREOTTI, R.
773 $tVeterinary Parasitology$gv. 221, p. 30-38, 2016
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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| Registros recuperados : 8 | |
| 4. |  | CIVIDANES, F. J.; BARBOSA, J. C.; IDE, S.; PERIOTO, N. W.; LARA, R. I. R. Faunistic analysis of Carabidae and Staphylinidae (Coleoptera) in five agroecosystems in northeastern São Paulo state, Brazil. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 8, p. 954-958, ago. 2009 Títulos em português: Análise faunística de Carabidae e Staphylinidae (Coleoptera) em cinco agroecossistemas da Região Nordeste do Estado de São Paulo.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 5. | | CIVIDANES, F. J.; MARTINS, I. C. F.; ARAUJO, E. S.; BARBOSA, J. C.; IDE, S.; PERIOTO, N. W. Faunistic analysis of Carabidae and Staphylinidae (Coleoptera) in five agroecosystems in the Northeastern region of the São Paulo state. In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.| Biblioteca(s): Embrapa Florestas. |
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| 6. | | FERNANDES, D. R. R.; GUIMARÃES, J. A.; ARAUJO, E. L.; LARA, R. I. R.; PERIOTO, N. W. Resultados preliminares da biodiversidade de himenópteros parasitóides coletados em área da caatinga em Mossoró, RN, Brasil. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 10., 2007, Brasília, DF. Inovar para preservar a vida: anais. Brasilia, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 7. | | FERNANDES, D. R. R.; GUIMARAES, J. A.; ARAUJO, E. L.; LARA, R. I. R.; PERIOTO, N. W. Survey of the Hymenoptera fauna in a "Caatinga" area in the state of Rio Grande do Norte, Northeastern Brazil. EntomoBrasilis, v. 7, n. 3, p. 211-215, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 8. | | FERNANDES, D. D. R.; VACARI, A. M.; ARAÚJO, E. L.; GUIMARÃES, J. A.; STRIKIS, P. C.; DEBORTOLI, S. A.; PERIOTO, N. W. Himenópteros parasitóides de moscas frugívoras (Diptera: Tephritoidea) associadas a frutos de abiu Pouteria caimito (Sapotaceae) em Jaboticabal, SP. In: REUNIÃO ANUAL DO INSTITUTO BIOLÓGICO, 21., 2008, São Paulo. Resumos... São Paulo: Instituto Biológico, 2008. p. 161 Publicado em : Biológico, São Paulo, v.70, n.2, p. 107-216, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| Registros recuperados : 8 | |
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