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Registro Completo |
Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
16/12/2021 |
Data da última atualização: |
17/12/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
HAILE, T.; CARDOSO, S. M.; RAPHAELLI, C. de O.; PEREIRA, O. R.; PEREIRA, E. dos S.; VIZZOTTO, M.; NORA, L.; ASFAW, A. A.; PERIASAMY, G.; KARIM, A. |
Afiliação: |
TESFAY HAILE, Mekelle University; SUSANA M. CARDOSO; CHIRLE DE OLIVEIRA RAPHAELLI; OLÍVIA R. PEREIRA; ELISA DOS SANTOS PEREIRA; MARCIA VIZZOTTO, CPACT; LEONARDO NORA; ADISSU ALEMAYEHU ASFAW, Mekelle University; GOMATHI PERIASAMY, Mekelle University; AMAN KARIM, Mekelle University. |
Título: |
Chemical Composition, Antioxidant Potential, and Blood Glucose Lowering Effect of Aqueous Extract and Essential Oil of Thymus Serrulatus Hochst. Ex Benth. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Frontiers in Pharmacology, v. 12, article 621536, April 2021. |
Idioma: |
Português |
Conteúdo: |
Thymus serrulatus, an endemic plant of Ethiopia, is traditionally used to cure various diseases and as a food ingredient. In the Ethiopian folk medicine, the decoction is orally taken as a remedy to treat diabetes and high blood pressure. The purpose of the present study was to evaluate the antioxidant and antihyperglycemic effects of the aqueous extract and of the essential oil of Thymus serrulatus. The chemical composition of the aqueous extract was determined by LC-MS and the essential oil was characterized by GC-MS analysis. Radical scavenging assays, namely scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH?), hydroxyl (?OH), and nitric oxide (?NO), were used as a first approach to screen the potential antioxidant abilities of the samples. Alpha-amylase and α-glucosidase inhibitory studies were also employed to evaluate the in vitro antihyperglycemic potential of the plant. The in vivo blood glucose lowering effect of the extracts was assessed using hypoglycemic activity and the oral glucose tolerance test in normal and in streptozotocin induced diabetic mice. When compared to the aqueous extract, the essential oil showed superior radical scavenging activity, particularly for ?NO, as well as greater inhibitory potency against α-amylase and α-glucosidase (IC50 0.01 mg/ml and 0.11 mg/ml, respectively). Both tested samples showed a statistically significant antihyperglycemic effect. The aqueous extract at 600 mg/kg exerted maximum antihyperglycemic activity (44.14%), followed by the essential oil (30.82%). Body weight and glucose tolerance parameters were also improved by the samples both in normal and diabetic mice. The findings of this study support the hypothesis that aqueous extract and essential oil of T. serrulatus are promising therapeutic agents. MenosThymus serrulatus, an endemic plant of Ethiopia, is traditionally used to cure various diseases and as a food ingredient. In the Ethiopian folk medicine, the decoction is orally taken as a remedy to treat diabetes and high blood pressure. The purpose of the present study was to evaluate the antioxidant and antihyperglycemic effects of the aqueous extract and of the essential oil of Thymus serrulatus. The chemical composition of the aqueous extract was determined by LC-MS and the essential oil was characterized by GC-MS analysis. Radical scavenging assays, namely scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH?), hydroxyl (?OH), and nitric oxide (?NO), were used as a first approach to screen the potential antioxidant abilities of the samples. Alpha-amylase and α-glucosidase inhibitory studies were also employed to evaluate the in vitro antihyperglycemic potential of the plant. The in vivo blood glucose lowering effect of the extracts was assessed using hypoglycemic activity and the oral glucose tolerance test in normal and in streptozotocin induced diabetic mice. When compared to the aqueous extract, the essential oil showed superior radical scavenging activity, particularly for ?NO, as well as greater inhibitory potency against α-amylase and α-glucosidase (IC50 0.01 mg/ml and 0.11 mg/ml, respectively). Both tested samples showed a statistically significant antihyperglycemic effect. The aqueous extract at 600 mg/kg exerted maximum antihyperglycemic activity (... Mostrar Tudo |
Palavras-Chave: |
Thymus serrulatus. |
Thesagro: |
Antioxidante; Extrato; Extrato Vegetal; Óleo Essencial. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/229329/1/9-HAILE.pdf
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Marc: |
LEADER 02681naa a2200289 a 4500 001 2138005 005 2021-12-17 008 2021 bl uuuu u00u1 u #d 100 1 $aHAILE, T. 245 $aChemical Composition, Antioxidant Potential, and Blood Glucose Lowering Effect of Aqueous Extract and Essential Oil of Thymus Serrulatus Hochst. Ex Benth.$h[electronic resource] 260 $c2021 520 $aThymus serrulatus, an endemic plant of Ethiopia, is traditionally used to cure various diseases and as a food ingredient. In the Ethiopian folk medicine, the decoction is orally taken as a remedy to treat diabetes and high blood pressure. The purpose of the present study was to evaluate the antioxidant and antihyperglycemic effects of the aqueous extract and of the essential oil of Thymus serrulatus. The chemical composition of the aqueous extract was determined by LC-MS and the essential oil was characterized by GC-MS analysis. Radical scavenging assays, namely scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH?), hydroxyl (?OH), and nitric oxide (?NO), were used as a first approach to screen the potential antioxidant abilities of the samples. Alpha-amylase and α-glucosidase inhibitory studies were also employed to evaluate the in vitro antihyperglycemic potential of the plant. The in vivo blood glucose lowering effect of the extracts was assessed using hypoglycemic activity and the oral glucose tolerance test in normal and in streptozotocin induced diabetic mice. When compared to the aqueous extract, the essential oil showed superior radical scavenging activity, particularly for ?NO, as well as greater inhibitory potency against α-amylase and α-glucosidase (IC50 0.01 mg/ml and 0.11 mg/ml, respectively). Both tested samples showed a statistically significant antihyperglycemic effect. The aqueous extract at 600 mg/kg exerted maximum antihyperglycemic activity (44.14%), followed by the essential oil (30.82%). Body weight and glucose tolerance parameters were also improved by the samples both in normal and diabetic mice. The findings of this study support the hypothesis that aqueous extract and essential oil of T. serrulatus are promising therapeutic agents. 650 $aAntioxidante 650 $aExtrato 650 $aExtrato Vegetal 650 $aÓleo Essencial 653 $aThymus serrulatus 700 1 $aCARDOSO, S. M. 700 1 $aRAPHAELLI, C. de O. 700 1 $aPEREIRA, O. R. 700 1 $aPEREIRA, E. dos S. 700 1 $aVIZZOTTO, M. 700 1 $aNORA, L. 700 1 $aASFAW, A. A. 700 1 $aPERIASAMY, G. 700 1 $aKARIM, A. 773 $tFrontiers in Pharmacology$gv. 12, article 621536, April 2021.
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Embrapa Clima Temperado (CPACT) |
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Registro Completo
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
29/07/2020 |
Data da última atualização: |
03/10/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
BERNARDO, C. das C.; PEREIRA JUNIOR, R. A.; LUZ, C.; MASCARIN, G. M.; FERNANDES, E. K. K. |
Afiliação: |
CÍNTIA DAS CHAGAS BERNARDO, UFG; RONALDO ALVES PERERIA JUNIOR, UFG; CHRISTIAN LUZ, UFG; GABRIEL MOURA MASCARIN, CNPMA; ÉVERTON KORT KAMP FERNANDES, UFG. |
Título: |
Differential susceptibility of blastospores and aerial conidia of entomopathogenic fungi to heat and UV-B stresses. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Fungal Biology, v. 124, n. 8, p. 714-722, 2020. |
DOI: |
https://doi.org/10.1016/j.funbio.2020.04.003 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: We investigated the comparative susceptibility to heat and UV-B radiation of blastospores and aerial conidia of Metarhizium spp. (Metarhizium robertsii IP 146, Metarhizium anisopliae s.l. IP 363 and Metarhizium acridum ARSEF 324) and Beauveria bassiana s.l. (IP 361 and CG 307). Conidia and blastospores were produced in solid or liquid Adámek-modified medium, respectively, and then exposed to heat (45 ± 0.2 °C) in a range of 0 (control) to 360 min; the susceptibility of fungal propagules to heat exposures was assessed to express relative viability. Similarly, both propagules of each isolate were also exposed to a range of 0 (control) to 8.1 kJ m?2 under artificial UV-B radiation. Our results showed that fungal isolates, propagule types and exposure time or dose of the stressor source play critical roles in fungal survival challenged with UV-B and heat. Conidia of ARSEF 324, IP 363, IP 146 and IP 361 exposed to heat survived significantly longer than their blastospores, except for blastospores of CG 307. Conidia and blastospores of IP 146 and IP 363 were equally tolerant to UV-B radiation. We claim that blastospores of certain isolates may be promising candidates to control arthropod pests in regions where heat and UV-B are limiting environmental factors. |
Palavras-Chave: |
Fungal propagule; Thermotolerance; UV tolerance. |
Thesagro: |
Calor; Controle Biológico; Fungo Para Controle Biológico; Raio Ultravioleta; Resistência a Temperatura. |
Thesaurus NAL: |
Abiotic stress; Beauveria; Biological control agents; Entomopathogenic fungi; Heat tolerance; Metarhizium; Ultraviolet radiation. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02408naa a2200361 a 4500 001 2124082 005 2020-10-03 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.funbio.2020.04.003$2DOI 100 1 $aBERNARDO, C. das C. 245 $aDifferential susceptibility of blastospores and aerial conidia of entomopathogenic fungi to heat and UV-B stresses.$h[electronic resource] 260 $c2020 520 $aAbstract: We investigated the comparative susceptibility to heat and UV-B radiation of blastospores and aerial conidia of Metarhizium spp. (Metarhizium robertsii IP 146, Metarhizium anisopliae s.l. IP 363 and Metarhizium acridum ARSEF 324) and Beauveria bassiana s.l. (IP 361 and CG 307). Conidia and blastospores were produced in solid or liquid Adámek-modified medium, respectively, and then exposed to heat (45 ± 0.2 °C) in a range of 0 (control) to 360 min; the susceptibility of fungal propagules to heat exposures was assessed to express relative viability. Similarly, both propagules of each isolate were also exposed to a range of 0 (control) to 8.1 kJ m?2 under artificial UV-B radiation. Our results showed that fungal isolates, propagule types and exposure time or dose of the stressor source play critical roles in fungal survival challenged with UV-B and heat. Conidia of ARSEF 324, IP 363, IP 146 and IP 361 exposed to heat survived significantly longer than their blastospores, except for blastospores of CG 307. Conidia and blastospores of IP 146 and IP 363 were equally tolerant to UV-B radiation. We claim that blastospores of certain isolates may be promising candidates to control arthropod pests in regions where heat and UV-B are limiting environmental factors. 650 $aAbiotic stress 650 $aBeauveria 650 $aBiological control agents 650 $aEntomopathogenic fungi 650 $aHeat tolerance 650 $aMetarhizium 650 $aUltraviolet radiation 650 $aCalor 650 $aControle Biológico 650 $aFungo Para Controle Biológico 650 $aRaio Ultravioleta 650 $aResistência a Temperatura 653 $aFungal propagule 653 $aThermotolerance 653 $aUV tolerance 700 1 $aPEREIRA JUNIOR, R. A. 700 1 $aLUZ, C. 700 1 $aMASCARIN, G. M. 700 1 $aFERNANDES, E. K. K. 773 $tFungal Biology$gv. 124, n. 8, p. 714-722, 2020.
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