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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
19/04/2023 |
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Data da última atualização: |
19/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RIEGER, J. S. G.; MANTOVANI, C.; SUNIGA, P. A. P.; PINTO, I. B.; SOUSA, G. A. A.; GAVA, D.; CANTAO, M. E.; SANTOS, L. R.; ARAÚJO, F. R.; ZANELLA, J. R. C. |
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Afiliação: |
DANIELLE GAVA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; JANICE REIS CIACCI ZANELLA, CNPSA. |
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Título: |
Standardization of ELISA with senecavirus A recombinant VP2 protein and its use in swine herds in Brazil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 22, n. 1, ed. gmr19118, 2023. |
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DOI: |
http://dx.doi.org/10.4238/gmr19118 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here was able to detect specific SVA antibodies in acute disease and recovered pigs, and no cross-reactivity with VSV was observed. This assay has potential as a useful tool for monitoring SVA infection and could help to improve disease diagnosis. MenosAbstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here wa... Mostrar Tudo |
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Thesagro: |
Diagnostico. |
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Thesaurus Nal: |
Senecavirus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1153239/1/final10100.pdf
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Marc: |
LEADER 02545naa a2200265 a 4500
001 2153239
005 2023-04-19
008 2023 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/gmr19118$2DOI
100 1 $aRIEGER, J. S. G.
245 $aStandardization of ELISA with senecavirus A recombinant VP2 protein and its use in swine herds in Brazil.$h[electronic resource]
260 $c2023
520 $aAbstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here was able to detect specific SVA antibodies in acute disease and recovered pigs, and no cross-reactivity with VSV was observed. This assay has potential as a useful tool for monitoring SVA infection and could help to improve disease diagnosis.
650 $aSenecavirus
650 $aDiagnostico
700 1 $aMANTOVANI, C.
700 1 $aSUNIGA, P. A. P.
700 1 $aPINTO, I. B.
700 1 $aSOUSA, G. A. A.
700 1 $aGAVA, D.
700 1 $aCANTAO, M. E.
700 1 $aSANTOS, L. R.
700 1 $aARAÚJO, F. R.
700 1 $aZANELLA, J. R. C.
773 $tGenetics and Molecular Research$gv. 22, n. 1, ed. gmr19118, 2023.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
10/01/2018 |
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Data da última atualização: |
12/12/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
BAGAGIM, J. B. C.; PEREIRA, W. de B.; SOUSA, J. S. C. de; SANTOS, M. E. P. dos; MOURA, M. S. B. de. |
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Afiliação: |
JOÃO BATISTA COELHO BAGAGIM, IF-Sertão CPZR, Petrolina, Pernambuco; WILTEMBERG DE BRITO PEREIRA, IF-Sertão CPZR, Petrolina, Pernambuco; JOSÉ SEBASTIÃO COSTA DE SOUSA, IF-Sertão CPZR, Petrolina, Pernambuco; MARIA ERICA PEREIRA DOS SANTOS, IF-Sertão CPZR, Petrolina, Pernambuco; MAGNA SOELMA BESERRA DE MOURA, CPATSA. |
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Título: |
Balanço hídrico climatológico normal para a estação agrometeorologica de Mandacaru localizada em Juazeiro-BA. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE AGROMETEOROLOGIA, 20; SIMPÓSIO DE MUDANÇAS CLIMÁTICAS E DESERTIFICAÇÃO NO SEMIÁRIDO BRASILEIRO, 5., 2017, Juazeiro, BA. A agrometeorologia na solução de problemas multiescala: anais. Petrolina: Embrapa Semiárido; Juazeiro: UNIVASF; Campinas: Sociedade Brasileira de Agrometeorologia, 2017. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
O balanço hídrico fornece a contabilidade de entradas e saídas de água em uma determinada região, tendo importância relevante para o planejamento da agricultura de sequeiro e do manejo de irrigação de áreas irrigadas. Sendo que no semiárido nordestino a agricultura de sequeiro é prevalecente, e para que a mesma tenha produções favoráveis é necessário ter um planejamento hídrico adequado. Além disso, é de suma importância o uso racional da água para um manejo eficiente, evitando assim danos como erosões, salinização e lixiviação de nutrientes. Desta forma objetivou-se com este trabalho determinar o balanço hídrico climatológico para a estação agrometeorológica convencional de Mandacaru localizada em Juazeiro-BA para o período de 1986-2015. Observou-se que a região apresenta deficiência hídrica em todos os meses, tendo quadra chuvosa compreendida entre os meses de dezembro a março. |
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Palavras-Chave: |
Agricultura de sequeiro; Estação agrometeorologica de Mandacaru; Natural resource. |
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Thesagro: |
Chuva; Clima; Deficiência Hídrica. |
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Thesaurus NAL: |
Climate; Rain. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/177295/1/Magna-17.pdf
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Marc: |
LEADER 01999nam a2200265 a 4500
001 2084855
005 2023-12-12
008 2017 bl uuuu u00u1 u #d
100 1 $aBAGAGIM, J. B. C.
245 $aBalanço hídrico climatológico normal para a estação agrometeorologica de Mandacaru localizada em Juazeiro-BA.
260 $aIn: CONGRESSO BRASILEIRO DE AGROMETEOROLOGIA, 20; SIMPÓSIO DE MUDANÇAS CLIMÁTICAS E DESERTIFICAÇÃO NO SEMIÁRIDO BRASILEIRO, 5., 2017, Juazeiro, BA. A agrometeorologia na solução de problemas multiescala: anais. Petrolina: Embrapa Semiárido; Juazeiro: UNIVASF; Campinas: Sociedade Brasileira de Agrometeorologia$c2017
300 $c1 CD-ROM.
520 $aO balanço hídrico fornece a contabilidade de entradas e saídas de água em uma determinada região, tendo importância relevante para o planejamento da agricultura de sequeiro e do manejo de irrigação de áreas irrigadas. Sendo que no semiárido nordestino a agricultura de sequeiro é prevalecente, e para que a mesma tenha produções favoráveis é necessário ter um planejamento hídrico adequado. Além disso, é de suma importância o uso racional da água para um manejo eficiente, evitando assim danos como erosões, salinização e lixiviação de nutrientes. Desta forma objetivou-se com este trabalho determinar o balanço hídrico climatológico para a estação agrometeorológica convencional de Mandacaru localizada em Juazeiro-BA para o período de 1986-2015. Observou-se que a região apresenta deficiência hídrica em todos os meses, tendo quadra chuvosa compreendida entre os meses de dezembro a março.
650 $aClimate
650 $aRain
650 $aChuva
650 $aClima
650 $aDeficiência Hídrica
653 $aAgricultura de sequeiro
653 $aEstação agrometeorologica de Mandacaru
653 $aNatural resource
700 1 $aPEREIRA, W. de B.
700 1 $aSOUSA, J. S. C. de
700 1 $aSANTOS, M. E. P. dos
700 1 $aMOURA, M. S. B. de
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