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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
17/09/2012 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FEDERICI, M. T.; PICCHI, S. C.; STUCHI, E. S.; FADEL, A. L.; LAIA, M. L.; LEMOS, M. V. F.; LEMOS, E. G. M. |
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Afiliação: |
MARÍA TERESA FEDERICI, INIA; SIMONE CRISTINA PICCHI, UNESP; EDUARDO SANCHES STUCHI, CNPMF; ANDRÉ LUIZ FADEL, ESALQ; MARCELO LUIZ LAIA, UFVJM; MANOEL VICTOR FRANCO LEMOS, UNESP; ELIANA GERTRUDES MACEDO LEMOS, UNESP. |
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Título: |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Electronic Journal of Biotechnology, 2012. |
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ISSN: |
0717-3458 |
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DOI: |
10.2225/vol15-issue3-fulltext-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosXylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increas... Mostrar Tudo |
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Palavras-Chave: |
Microarray. |
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Thesagro: |
Xylella Fastidiosa. |
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Thesaurus Nal: |
Citrus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02405naa a2200253 a 4500
001 1933882
005 2023-05-25
008 2012 bl uuuu u00u1 u #d
022 $a0717-3458
024 7 $a10.2225/vol15-issue3-fulltext-4$2DOI
100 1 $aFEDERICI, M. T.
245 $aXylella fastidiosa$bAn in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis.$h[electronic resource]
260 $c2012
520 $aXylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels.
650 $aCitrus
650 $aXylella Fastidiosa
653 $aMicroarray
700 1 $aPICCHI, S. C.
700 1 $aSTUCHI, E. S.
700 1 $aFADEL, A. L.
700 1 $aLAIA, M. L.
700 1 $aLEMOS, M. V. F.
700 1 $aLEMOS, E. G. M.
773 $tElectronic Journal of Biotechnology, 2012.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Classificação |
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URL |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
18/02/1998 |
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Data da última atualização: |
10/02/2012 |
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Autoria: |
HERNANDEZ FILHO, P.; PONZONI, F. J.; PEREIRA, M. N.; POTT, A.; POTT, V. J.; SILVA, M. P. da. |
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Afiliação: |
Instituto Nacional de Pesquisas Espaciais. Divisao de Sensoriamento Remoto (Sao Jose dos Campos, SP); EMBRAPA. Centro de Pesquisa Agropecuaria do Pantanal (Corumba, MS). |
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Título: |
Mapeamento da vegetacao e de uso da terra de parte da bacia do Alto Taquari, MS considerando o procedimento de analise visual de imagens TM/Landsat e HRV/Spot. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
In: ENCONTRO SOBRE SENSORIAMENTO REMOTO APLICADO A ESTUDOS NO PANTANAL, 1995, Corumba. Livro de resumos... Sao Jose dos Campos: INPE, 1995. |
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Páginas: |
p.137-139. |
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Idioma: |
Português |
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Conteúdo: |
Nos ultimos 20 anos, observou-se a intensificacao dos processos erosivo na bacia do Alto Taquari, consequencia da expansao da fronteira agricola, a qual foi conduzida sem a definicao de um planejamento que contemplasse nao so as atividades agropecuarias como tambem a questao ambiental. Como resultado dessa situacao, esta regiao apresenta uma aceleracao da erosao na parte do planalto, onde tem sido concentradas as atividades agricolas. O objetivo deste trabalho e avaliar a contribuicao do sensoriamento remoto orbital, no levantamento da cobertura vegetal e do uso e ocupacao da terra na regiao do planalto e da planicie do Alto Taquari, localizada no Estado do Mato Grosso do Sul, considerando o procedimento de analise visual de Imagens de satelite, para contribuir na definicao de acoes corretivas para o ordenamento territorial. |
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Palavras-Chave: |
Mapeamento; Mapping. |
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Thesagro: |
Sensoriamento Remoto; Uso da Terra; Vegetação. |
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Thesaurus NAL: |
land use; Pantanal; remote sensing; vegetation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01791naa a2200301 a 4500
001 1792088
005 2012-02-10
008 1995 bl uuuu u00u1 u #d
100 1 $aHERNANDEZ FILHO, P.
245 $aMapeamento da vegetacao e de uso da terra de parte da bacia do Alto Taquari, MS considerando o procedimento de analise visual de imagens TM/Landsat e HRV/Spot.
260 $c1995
300 $ap.137-139.
520 $aNos ultimos 20 anos, observou-se a intensificacao dos processos erosivo na bacia do Alto Taquari, consequencia da expansao da fronteira agricola, a qual foi conduzida sem a definicao de um planejamento que contemplasse nao so as atividades agropecuarias como tambem a questao ambiental. Como resultado dessa situacao, esta regiao apresenta uma aceleracao da erosao na parte do planalto, onde tem sido concentradas as atividades agricolas. O objetivo deste trabalho e avaliar a contribuicao do sensoriamento remoto orbital, no levantamento da cobertura vegetal e do uso e ocupacao da terra na regiao do planalto e da planicie do Alto Taquari, localizada no Estado do Mato Grosso do Sul, considerando o procedimento de analise visual de Imagens de satelite, para contribuir na definicao de acoes corretivas para o ordenamento territorial.
650 $aland use
650 $aPantanal
650 $aremote sensing
650 $avegetation
650 $aSensoriamento Remoto
650 $aUso da Terra
650 $aVegetação
653 $aMapeamento
653 $aMapping
700 1 $aPONZONI, F. J.
700 1 $aPEREIRA, M. N.
700 1 $aPOTT, A.
700 1 $aPOTT, V. J.
700 1 $aSILVA, M. P. da
773 $tIn: ENCONTRO SOBRE SENSORIAMENTO REMOTO APLICADO A ESTUDOS NO PANTANAL, 1995, Corumba. Livro de resumos... Sao Jose dos Campos: INPE, 1995.
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Embrapa Pantanal (CPAP) |
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