Data/hora: 17/06/2024 15:49:37

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	11/02/2014	
Data da última atualização: 	05/02/2024	
Autoria: 	PEREIRA, M. M.; RAPOSO, N. R. B.; BRAYNER, R.; TEIXEIRA, E. M.; OLIVEIRA, V.; QUINTAO, C. C. R.; CAMARGO, L. S. de A.; MATTOSO, L. H. C.; BRANDAO, H. de M.	
Afiliação: 	M. M. PEREIRA, UFJF; N. R. B. RAPOSO, UFJF; R. BRAYNER, University of Paris Diderot, Paris; E. M. TEIXEIRA; V. OLIVEIRA, UFJF; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA; HUMBERTO DE MELLO BRANDAO, CNPGL.	
Título: 	Cytotoxicity and expression of genes involved in the cellular stress response and apoptosis in mammalian fibroblast exposed to cotton cellulose nanofibers.	
Ano de publicação: 	2013	
Fonte/Imprenta: 	Nanotechnology, v. 24, n. 7, 075103, 2013.	
DOI: 	https://doi.org/10.1088/0957-4484/24/7/075103	
Idioma: 	Inglês	
Conteúdo: 	Cellulose nanofibers (CNF) have mechanical properties that make them very attractive for applications in the construction of polymeric matrices, drug delivery and tissue engineering. However, little is known about their impact on mammalian cells. The objective of this study was to evaluate the cytotoxicity of CNF and their effect on gene expression of fibroblasts cultured in vitro. The morphology of CNF was analyzed by transmission electron microscopy and the surface charge by Zeta potential. Cell viability was analyzed by flow cytometry assay and gene expression of biomarkers focused on cell stress response such as Heat shock protein 70.1 (HSP70.1) and Peroxiredoxin 1 (PRDX1) and apoptosis as B-cell leukemia (BCL-2) and BCL-2 associated X protein (BAX) by RT-PCR assay. Low concentrations of CNF (0.02-100 μg ml(-1)) did not cause cell death; however, at concentrations above 200 μg ml(-1), the nanofibers significantly decreased cell viability (86.41 ± 5.37%). The exposure to high concentrations of CNF (2000 and 5000 μg ml(-1)) resulted in increased HSP70.1, PRDX1 and BAX gene expression. The current study concludes that, under the conditions tested, high concentrations (2000 and 5000 μg ml(-1)) of CNF cause decreased cell viability and affect the expression of stress- and apoptosis-associated molecular markers.	
Palavras-Chave: 	Animals Apoptosis; Apoptosis/genetics; Cattle Cellulose/pharmacology; Drug effects; Fibroblasts/cytology; Fibroblasts/drug.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (21102-1) - UPC - AP - DD -  -  -  - DIS