Data/hora: 06/06/2024 13:02:12

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 01/08/2016
Data da última atualização: | 01/08/2016
Autoria: | OBERLENDER, G.; RUIZ LÓPEZ, S.; DE ONDIZ SÁNCHEZ, A. D.; VIEIRA, L. A.; PEREIRA, M. B.; SILVA, L. de F.; ZANGERONIMO, M. G.; MURGAS, L. D. S.
Afiliação: | GUILHERME OBERLENDER, IFSULDEMINAS; SALVARDOR RUIZ LÓPEZ, University of Murcia; AITOR D. DE ORDIZ SÁNCHEZ, University of Zulia; LUIS A. VIEIRA, PPGCV/UECE; MARIANE BARRETO PEREIRA, IFSULDEMINAS; LUANY DE FÁTIMA SILVA, IFSULDEMINAS; MÁRCIO G. ZANGERONIMO, DMV/UFLA; LUIS D. S. MURGAS, DMV/UFLA.
Título: | In vitro fertilization of porcine oocytes is affected by spermatic coincubation time.
Ano de publicação: | 2016
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Brasília, DF, v. 36 (supl. 1), p. 89-93, jun. 2016.
Idioma: | Inglês
Conteúdo: | The aim was to study the effects of different gamete coincubation times on porcine in vitro fertilization (IVF), and to verify whether efficiency could be improved by reducing oocyte exposure time to spermatozoa during IVF. In groups of 50, a total of 508 immature cumulus-oocyte complexes (COCs) were matured in NCSU-37 medium. The COCs were cultured for 44 hours and then inseminated with in natura semen (2,000 spermatozoa/ oocyte). The sperm and oocytes were coincubated according to the following treatments (T): T1 = oocytes exposed to spermatozoa for one hour (173 oocytes), T2 = oocytes exposed to spermatozoa for two hours (170 oocytes), and T3 = oocytes exposed to spermatozoa for three hours (165 oocytes). After these coincubation periods, the oocytes were washed in fertilization medium (TALP medium) to remove spermatozoa not bound to the zona pellucida and cultured in another similar medium (containing no sperm). Eighteen to twenty hours after fertilization, the putative zygotes were stained in Hoechst-33342 to evaluate the IVF results. The penetration rate was higher (P<0.05) after two hours of coincubation time than it was for one or three hours. Furthermore, 68.60% of the ova coincubated with the spermatozoa for two hours were monospermic. The oocytes exposed to spermatozoa for one hour (T1) presented a higher (P<0.01) rate of polyspermy than those in T2 and T3. Fertilization performance (%) did not differ (P>0.05) between oocytes exposed to spermatozoa for one (T1) and three hours (T3). However, optimum (P=0.048) results were obtained after two hours of coincubation, when the rate of fertilization performance was 50.16±8.52%. The number of penetrated sperm per oocyte, as well as male pronucleus formation, did not differ (P>0.05) between the treatments evaluated. Under these assay conditions, especially in relation to the sperm concentration used, gamete coincubation for a period of two hours appears to be optimal for monospermy and fertilization performance. Thus, it is the optimal time period for obtaining a large number of pig embryos capable of normal development.
Palavras-Chave: | Coincubation time; Eficiência da fertilização; Fertilização in vitro; Fetilization performance; Ovócito; Pig; Tempo de coincubação.
Thesagro: | Suíno.
Thesaurus Nal: | In vitro fertilization; Oocytes.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/145959/1/In-vitro-fertilization.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (59814-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS