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| 27. |  | BRAGA, V. C.; CAMILLO, J.; PEREIRA, J. E. S. Parâmetros biométricos de diásporos de dendezeiro [Elaeis guineensis (JACQ.) VAR. DURA], como subsídio para a conservação de germoplasma. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. CD-ROM| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/03/2017 |
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Data da última atualização: |
29/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
BALZON, T. A.; LUIS, Z. G.; PEREIRA, J. E. S. |
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Afiliação: |
Talita Aparecida Balzon; Zanderluce Gomes Luis, UnB; JONNY EVERSON SCHERWINSKI PEREIRA, Cenargen. |
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Título: |
New approaches to improve the efficiency of somatic embryogenesis in oil palm (Elaeis guineensis Jacq.) from mature zygotic embryos. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In Vitro Cellular e Developmental Biology, v. 49, p. 41-50, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
We developed an efficient and simple system for inducing somatic embryogenesis and regenerating plantlets from mature zygotic embryos of oil palm. Embryogenic calli were induced from mature zygotic embryos of oil palm on modified Murashige and Skoog medium with 2,4-dichlorophenoxyacetic acid or picloram, alone or in combination with activated charcoal. The greatest frequency of embryogenic callus induction (97.5%) was obtained by culturing mature zygotic embryos on callus induction medium with 450 μM picloram and 2.5 gL−1 activated charcoal. Embryogenic calli proliferated on a medium with a reduced concentration of picloram. Embryogenic calli were then subcultured on a medium supplemented with 12.3 μM 2-isopentenyladenine and 0.54 μM naphthaleneacetic acid, with subcultures at 4-wk intervals. Somatic embryos were regenerated on a medium with Murashige and Skoog macro- and micronutrients at halfstrength concentrations supplemented with 20 gL−1 sucrose, 2.5 gL−1 activated charcoal, and 2.5 gL−1 Phytagel. Detailed histological analysis revealed that somatic embryogenesis followed an indirect pathway. Primary calli were observed after 4?6 wk of culture and progressed to embryogenic calli at 12 wk. Embryogenic cells exhibited dense protoplasm, a high nucleoplasmic ratio, and small starch grains. Proembryos, which seemed to have a multicellular origin, formed after 16?20 wk of culture and successive cell divisions. Differentiated somatic embryos had a haustorium, a plumule, and the first and second foliar sheaths. In differentiated embryos, the radicular protrusion was not apparent because it generally does not appear until after the first true leaves emerge. MenosWe developed an efficient and simple system for inducing somatic embryogenesis and regenerating plantlets from mature zygotic embryos of oil palm. Embryogenic calli were induced from mature zygotic embryos of oil palm on modified Murashige and Skoog medium with 2,4-dichlorophenoxyacetic acid or picloram, alone or in combination with activated charcoal. The greatest frequency of embryogenic callus induction (97.5%) was obtained by culturing mature zygotic embryos on callus induction medium with 450 μM picloram and 2.5 gL−1 activated charcoal. Embryogenic calli proliferated on a medium with a reduced concentration of picloram. Embryogenic calli were then subcultured on a medium supplemented with 12.3 μM 2-isopentenyladenine and 0.54 μM naphthaleneacetic acid, with subcultures at 4-wk intervals. Somatic embryos were regenerated on a medium with Murashige and Skoog macro- and micronutrients at halfstrength concentrations supplemented with 20 gL−1 sucrose, 2.5 gL−1 activated charcoal, and 2.5 gL−1 Phytagel. Detailed histological analysis revealed that somatic embryogenesis followed an indirect pathway. Primary calli were observed after 4?6 wk of culture and progressed to embryogenic calli at 12 wk. Embryogenic cells exhibited dense protoplasm, a high nucleoplasmic ratio, and small starch grains. Proembryos, which seemed to have a multicellular origin, formed after 16?20 wk of culture and successive cell divisions. Differentiated somatic embr... Mostrar Tudo |
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Palavras-Chave: |
Zygotic embryo. |
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Thesaurus NAL: |
morphogenesis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/180927/1/Balzon2013-Article-NewApproachesToImproveTheEffic.pdf
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Marc: |
LEADER 02287naa a2200169 a 4500
001 2067490
005 2023-03-29
008 2013 bl uuuu u00u1 u #d
100 1 $aBALZON, T. A.
245 $aNew approaches to improve the efficiency of somatic embryogenesis in oil palm (Elaeis guineensis Jacq.) from mature zygotic embryos.$h[electronic resource]
260 $c2013
520 $aWe developed an efficient and simple system for inducing somatic embryogenesis and regenerating plantlets from mature zygotic embryos of oil palm. Embryogenic calli were induced from mature zygotic embryos of oil palm on modified Murashige and Skoog medium with 2,4-dichlorophenoxyacetic acid or picloram, alone or in combination with activated charcoal. The greatest frequency of embryogenic callus induction (97.5%) was obtained by culturing mature zygotic embryos on callus induction medium with 450 μM picloram and 2.5 gL−1 activated charcoal. Embryogenic calli proliferated on a medium with a reduced concentration of picloram. Embryogenic calli were then subcultured on a medium supplemented with 12.3 μM 2-isopentenyladenine and 0.54 μM naphthaleneacetic acid, with subcultures at 4-wk intervals. Somatic embryos were regenerated on a medium with Murashige and Skoog macro- and micronutrients at halfstrength concentrations supplemented with 20 gL−1 sucrose, 2.5 gL−1 activated charcoal, and 2.5 gL−1 Phytagel. Detailed histological analysis revealed that somatic embryogenesis followed an indirect pathway. Primary calli were observed after 4?6 wk of culture and progressed to embryogenic calli at 12 wk. Embryogenic cells exhibited dense protoplasm, a high nucleoplasmic ratio, and small starch grains. Proembryos, which seemed to have a multicellular origin, formed after 16?20 wk of culture and successive cell divisions. Differentiated somatic embryos had a haustorium, a plumule, and the first and second foliar sheaths. In differentiated embryos, the radicular protrusion was not apparent because it generally does not appear until after the first true leaves emerge.
650 $amorphogenesis
653 $aZygotic embryo
700 1 $aLUIS, Z. G.
700 1 $aPEREIRA, J. E. S.
773 $tIn Vitro Cellular e Developmental Biology$gv. 49, p. 41-50, 2013.
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