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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
19/12/2017 |
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Data da última atualização: |
21/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
REIS, A. N. dos; MIRANDA, M. dos S.; SILVA, L. K. X.; SILVA, A. O. A. da; SOUSA, J. S. de; MORAIS, E. de; GARCIA, A. R.; DOMINGUES, S. F. S.; SILVA, J. K. do R. da; RIBEIRO, H. F. L. |
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Afiliação: |
Adriana Novaes dos Reis, UFPA; Moysés dos Santos Miranda, UFPA; Lílian Kátia Ximenes Silva, UNAMA; Aluizio Otavio Almeida da Silva, UFPA; José Silva de Sousa, UFPA; Eziquiel de Morais, IFPA; ALEXANDRE ROSSETTO GARCIA, CPPSE; Sheyla Farhayldes Souza Domingues, UFPA; Joyce Kelly do Rosário da Silva, UFPA; Haroldo Francisco Lobato Ribeiro, UFRA. |
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Título: |
Comparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 38, n. 6, p. 3613-3628, 2017. |
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DOI: |
10.5433/1679-0359.2017v38n6p3613 |
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Idioma: |
Inglês |
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Conteúdo: |
For artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evaluated sperm membrane integrity with the fluorescent probes PI, FITC-PSA and JC-1 using flow cytometry. The data were submitted to ANOVA, and the results were compared by Tukey?s test at a significance of 5%. In the first experiment, the extender TES-TRIS showed better results for the various characteristics evaluated compared to ACP-112® and ACP-112®-Milk (P < 0.05), demonstrating greater protection of the buffalo sperm structures during cryopreservation. In the second experiment, the treatments with different concentrations of Lippia origanoides essential oil extract showed no differences among the assessed variables regarding the protection of sperm structures during cryopreservation (P > 0.05). Based on these data, we demonstrated the beneficial effects of TES-TRIS for post-thaw buffalo sperm quality; however, no protective effect was observed for buffalo sperm cryopreserved with the different tested concentrations of Lippia origanoides extract oil. MenosFor artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evalu... Mostrar Tudo |
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Palavras-Chave: |
Powdered Coconut Water. |
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Thesaurus Nal: |
cryopreservation; Lippia. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169304/1/REIS-et-al-2016-Comparative-Evaluation-Between-TES-TRIS-and-ACP-112.pdf
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Marc: |
LEADER 03425naa a2200277 a 4500
001 2082949
005 2019-05-21
008 2017 bl uuuu u00u1 u #d
024 7 $a10.5433/1679-0359.2017v38n6p3613$2DOI
100 1 $aREIS, A. N. dos
245 $aComparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm.$h[electronic resource]
260 $c2017
520 $aFor artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evaluated sperm membrane integrity with the fluorescent probes PI, FITC-PSA and JC-1 using flow cytometry. The data were submitted to ANOVA, and the results were compared by Tukey?s test at a significance of 5%. In the first experiment, the extender TES-TRIS showed better results for the various characteristics evaluated compared to ACP-112® and ACP-112®-Milk (P < 0.05), demonstrating greater protection of the buffalo sperm structures during cryopreservation. In the second experiment, the treatments with different concentrations of Lippia origanoides essential oil extract showed no differences among the assessed variables regarding the protection of sperm structures during cryopreservation (P > 0.05). Based on these data, we demonstrated the beneficial effects of TES-TRIS for post-thaw buffalo sperm quality; however, no protective effect was observed for buffalo sperm cryopreserved with the different tested concentrations of Lippia origanoides extract oil.
650 $acryopreservation
650 $aLippia
653 $aPowdered Coconut Water
700 1 $aMIRANDA, M. dos S.
700 1 $aSILVA, L. K. X.
700 1 $aSILVA, A. O. A. da
700 1 $aSOUSA, J. S. de
700 1 $aMORAIS, E. de
700 1 $aGARCIA, A. R.
700 1 $aDOMINGUES, S. F. S.
700 1 $aSILVA, J. K. do R. da
700 1 $aRIBEIRO, H. F. L.
773 $tSemina: Ciências Agrárias, Londrina$gv. 38, n. 6, p. 3613-3628, 2017.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Acre; Embrapa Amapá. |
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Data corrente: |
31/10/2011 |
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Data da última atualização: |
16/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
CUNHA, A. C.; SILVA, R. A. da; PEREIRA, J. D. B.; SANTOS, R. S. |
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Afiliação: |
ALAN CAVALCANTI DA CUNHA, UNIFAP; RICARDO ADAIME DA SILVA, CPAF-AP; JÚLIA DANIELA BRAGA PEREIRA, UNIFAP; RODRIGO SOUZA SANTOS, CPAF-AC. |
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Título: |
Efeito da espessura da polpa, tamanho e peso de frutos de taperebá (Spondias mombin L.) sobre o parasitismo natural (Hymenoptera: Braconidae) em moscas-das-frutas (Diptera: Tephritadae). |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Revista de Agricultura, Piracicaba, v. 86, n. 2, p. 125-133, 2011. |
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Idioma: |
Português |
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Conteúdo: |
Os frutos do taperebazeiro (Spondias mombin L.) são muito apreciados pelas comunidades amazônicas, tanto para consumo in natura quanto em formas processadas. Do ponto de vista ecológico, é uma espécie que exerce importante função como reservatório de parasitóides (Hymenoptera) de moscas-das-frutas (Diptera: Tephritidae). de maniera geral, seus frutos são desuniformes em tamanho, peso e espessura de polpa. O objetivo deste trabalho foi verificar se esses parâmetros interferem na incidência de parasitóides de moscas-das-frutas. Foram coletados 75 frutos maduros (cada fruto individualmente representou uma amostra) de um taperebazeiro na área urbana do município de Macapá, Amapá. Os parâmetros analisados foram: peso dos frutos, maior e menor diâmetro dos frutos, espessura de polpa, índice de infestação de frutos por moscas-das-frutas e percentual de parasitismo. Não foi observada a existência de uma função matemática do tipo "decaimento exponencial" que ralcionasse os parâmetros avaliados ao percentual de parasitismo. Houve, porém, uma tendência de decréscimo linear, com baixa significância estatística. |
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Palavras-Chave: |
Amapá; Amazônia Oriental; Moscas de la fruta; Plagas de plantas; Taperebá. |
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Thesagro: |
Cajá; Características Agronômicas; Mosca das frutas; Parasitismo; Praga de planta; Spondias mombin. |
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Thesaurus NAL: |
Agronomic traits; Fruit flies; Parasitism; Plant pests. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/159903/1/23966.pdf
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Marc: |
LEADER 02206naa a2200337 a 4500
001 2139489
005 2022-05-16
008 2011 bl uuuu u00u1 u #d
100 1 $aCUNHA, A. C.
245 $aEfeito da espessura da polpa, tamanho e peso de frutos de taperebá (Spondias mombin L.) sobre o parasitismo natural (Hymenoptera$bBraconidae) em moscas-das-frutas (Diptera: Tephritadae).$h[electronic resource]
260 $c2011
520 $aOs frutos do taperebazeiro (Spondias mombin L.) são muito apreciados pelas comunidades amazônicas, tanto para consumo in natura quanto em formas processadas. Do ponto de vista ecológico, é uma espécie que exerce importante função como reservatório de parasitóides (Hymenoptera) de moscas-das-frutas (Diptera: Tephritidae). de maniera geral, seus frutos são desuniformes em tamanho, peso e espessura de polpa. O objetivo deste trabalho foi verificar se esses parâmetros interferem na incidência de parasitóides de moscas-das-frutas. Foram coletados 75 frutos maduros (cada fruto individualmente representou uma amostra) de um taperebazeiro na área urbana do município de Macapá, Amapá. Os parâmetros analisados foram: peso dos frutos, maior e menor diâmetro dos frutos, espessura de polpa, índice de infestação de frutos por moscas-das-frutas e percentual de parasitismo. Não foi observada a existência de uma função matemática do tipo "decaimento exponencial" que ralcionasse os parâmetros avaliados ao percentual de parasitismo. Houve, porém, uma tendência de decréscimo linear, com baixa significância estatística.
650 $aAgronomic traits
650 $aFruit flies
650 $aParasitism
650 $aPlant pests
650 $aCajá
650 $aCaracterísticas Agronômicas
650 $aMosca das frutas
650 $aParasitismo
650 $aPraga de planta
650 $aSpondias mombin
653 $aAmapá
653 $aAmazônia Oriental
653 $aMoscas de la fruta
653 $aPlagas de plantas
653 $aTaperebá
700 1 $aSILVA, R. A. da
700 1 $aPEREIRA, J. D. B.
700 1 $aSANTOS, R. S.
773 $tRevista de Agricultura, Piracicaba$gv. 86, n. 2, p. 125-133, 2011.
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Embrapa Amapá (CPAF-AP) |
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