Data/hora: 16/06/2024 00:54:19

Biblioteca(s): | Embrapa Gado de Corte.
Data corrente: | 03/05/2004
Data da última atualização: | 03/05/2004
Autoria: | RODRIGUES-OTUBO, B. M.; PENTEADO, M. I. de O.; VALLE, C. B. do.
Afiliação: | EMPAER, (Campo Grande, MS).
Título: | Embryo rescue of interspecific hybrids of Brachiaria spp.
Ano de publicação: | 2000
Fonte/Imprenta: | Plant Cell, Tissue and Organ Culture, Hague, v. 61, n. 3, p. 175-182, Jan. 2000.
Idioma: | Inglês
Notas: | CNPGC.
Conteúdo: | Age of explant and six different media were evaluated with the objective of regenerating higher numbers of interspecific hybrids between sexual and apomictic Brachiaria. Immature embryos of 7?8, 9?10 and 11?12 days after pollination (DAP), from artificial hybridization between Brachiaria ruziziensis (R) as female parent, and B. brizantha (B) or B. decumbens (D) as male parent, were cultured in modified MS media (M4) ? supplemented with different combinations of growth regulators and vitamins. Embryos cultured 9?12 DAP showed high percentage (85?100%) of germination for all the crosses examined. Germination and survival rates varied according to accessions within crosses. Six different media (all modified MS with different growth regulators and vitamins) were tested with the objective of inducing multiple shoots from 7 to 10 DAP embryos, from crosses between R × B. The media M1, supplemented with Kinetin (13.94 µM) and NAA (5.37 µM), and media M3, supplemented with BA (4.44 µM and IAA 2.85 µM), regenerated adventitious shoots and calli about 30?40 days after inoculation. The highest multiplication rate observed was 2.85 shoots per explant in media M1, 60?70 days after culturing. Two other media, M6, supplemented with 2,4-D (13.57 µM) and M2, with 2,4-D (9.05 µM) and BA (8.87 µM) exclusively induced the formation of calli. The described protocols proved to be efficient in regenerating healthy seedlings from immature embryos of interspecific hybrids in Brachiaria.
Palavras-Chave: | Embryo; Regeneration.
Thesagro: | Biotecnologia; Brachiaria; Cultura de Tecido; Embrião; Hibridação; Melhoramento Genético Vegetal; Regeneração.
Thesaurus Nal: | biotechnology; cell culture; interspecific hybridization; plant breeding.
Categoria do assunto: | --
Registro original: | Embrapa Gado de Corte (CNPGC)
Exemplares: | CNPGC - (10473-1) - UPC - SP - -- - 446/12 -  - 446/12 - DIS