Data/hora: 20/06/2024 12:48:38

Biblioteca(s): 	Embrapa Clima Temperado; Embrapa Rondônia.	
Tipo da produção científica: 	Artigo em Periódico Indexado	
Circulação/Nível: 	A - 2	
Data corrente: 	08/02/2017	
Data da última atualização: 	10/11/2021	
Autoria: 	CAMPOS, F. T. de; RINCON, J. A. A.; ACOSTA, D. A. V.; SILVEIRA, P. A. S.; PRADIEÉ, J.; CORRÊA, M. N.; GASPERIN, B. G.; PFEIFER, L. F. M.; BARROS, C. C.; PEGORARO, L. M. C.; SCHNEIDER, A.	
Afiliação: 	Felipe Terres de Campos, UFPel; Joao Aveiro Alvarado Rincon, UFPel; Diego Andres Velasco Acosta, CORPOICA; Pedro Augusto Silva Silveira, UFPel; Jorgea Pradieé, Embrapa Clima Temperado; Marcio Nunes Corrêa, UFPel; Bernardo Garziera Gasperin, UFPel; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-Rondonia; Carlos Castilho Barros, UFPel; LIGIA MARGARETH CANTARELLI PEGORARO, CPACT; Augusto Schneider, UFPel.	
Título: 	The acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle.	
Ano de publicação: 	2017	
Fonte/Imprenta: 	Theriogenology, v. 89, p. 244 - 249, 2017.	
Idioma: 	Inglês	
Conteúdo: 	The aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 &#956;g/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells.	
Palavras-Chave: 	Esteroidogênese; PON1.	
Thesagro: 	Colesterol; Inflamação.	
Thesaurus Nal: 	cholesterol; inflammation; steroidogenesis.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/227597/1/cpafro-17770.pdf	
Registro original: 	Embrapa Rondônia (CPAF-RO)	
Exemplares: 	CPAF-RO - (17770-1) - UPC - AP - DD -  -  -  - DIS
CPACT - (19802-1) - UPC - AP - DD -  -  -  - DIS