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| 15. |  | PEDROSA, F. O.; HUNGRIA, M.; YATES, G.; NEWTON, W. E. (ed.). Nitrogen fixation from molecules to crop productivity. Dordrecht: Kluwer Academic, 2000. 669 p. (Current Plant Science and Biotechnology in Agriculture, v.38). Apresentado no "12th International Congress on Nitrogen Fixation, Foz do Iguacu, Parana, Brazil, September 12-17, 1999".| Biblioteca(s): Embrapa Agrobiologia; Embrapa Cerrados; Embrapa Meio Ambiente; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Trigo. |
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| 18. | | BALDANI, V. L.; BALDANI, J. I.; PEDROSA, F. O.; FARIA, S. M.; DOBEREINER, J. Formação de estruturas "nodule-like" em plântulas de trigo tratadas com 2,4 D e inoculadas com Azospirillum spp. In: FEIRA E CONGRESSO NACIONAL DE BIOTECNOLOGIA, 2., 1991, São Paulo. Resumos... São Paulo: Associaçãoo Brasileira de Empresas de Biotecnologia, 1991. p. 59| Biblioteca(s): Embrapa Agrobiologia; Embrapa Trigo. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
01/10/2002 |
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Data da última atualização: |
01/10/2002 |
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Autoria: |
RAMOS, H. J. O.; RONCATO-MACCARI, L.D.B.; SOUZA, E. M.; SOARES-RAMOS, J.R.L.; HUNGRIA, M.; PEDROSA, F. O. |
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Título: |
Monitoring Azospirillum-wheat interactions using the gfp and gusA genes constitutively expressed from a new broad-host range vector. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Journal of Biotechnology, Amsterdam, v. 97, n. 3, p. 243-252, Aug. 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
To monitor the colonization of wheat roots by Azospirillum brasilense, we constructed several plasmids based on the pBBR1replicon expressing the gfp and gusA genes constitutively. Both genes were placed under control of the gentamycin resistance
gene promoter resulting in high levels of expression in Escherichia coli and A. brasilense. The constructed plasmids were stably maintained in A. brasilense strains even in the absence of selective pressure. The colonization of wheat plants grown under controlled conditions in sterilized vermiculite by A. brasilense strain FP2 (aSp7-derivative) transconjugants containing these plasmids was monitored. Bacteria expressing GFP were easily observed in fresh plant material by fluorescence microscopy. Cell aggregates and single bacteria were visualized on the surfaces of young root zones, such as roots hairs and lateral roots. Large cellular clumps were observed at the points of lateral root emergence or at intercellular spaces of root epidermal cells 30 days after inoculation. Although we failed to detected bacteria in internal cortical and xylem tissues of wheat
roots, the initial stage of endophytic colonization by A. brasilense may involve the sites detected in this work.
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Categoria do assunto: |
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Marc: |
LEADER 01825naa a2200181 a 4500
001 1463274
005 2002-10-01
008 2002 bl --- 0-- u #d
100 1 $aRAMOS, H. J. O.
245 $aMonitoring Azospirillum-wheat interactions using the gfp and gusA genes constitutively expressed from a new broad-host range vector.
260 $c2002
520 $aTo monitor the colonization of wheat roots by Azospirillum brasilense, we constructed several plasmids based on the pBBR1replicon expressing the gfp and gusA genes constitutively. Both genes were placed under control of the gentamycin resistance gene promoter resulting in high levels of expression in Escherichia coli and A. brasilense. The constructed plasmids were stably maintained in A. brasilense strains even in the absence of selective pressure. The colonization of wheat plants grown under controlled conditions in sterilized vermiculite by A. brasilense strain FP2 (aSp7-derivative) transconjugants containing these plasmids was monitored. Bacteria expressing GFP were easily observed in fresh plant material by fluorescence microscopy. Cell aggregates and single bacteria were visualized on the surfaces of young root zones, such as roots hairs and lateral roots. Large cellular clumps were observed at the points of lateral root emergence or at intercellular spaces of root epidermal cells 30 days after inoculation. Although we failed to detected bacteria in internal cortical and xylem tissues of wheat roots, the initial stage of endophytic colonization by A. brasilense may involve the sites detected in this work.
700 1 $aRONCATO-MACCARI, L.D.B.
700 1 $aSOUZA, E. M.
700 1 $aSOARES-RAMOS, J.R.L.
700 1 $aHUNGRIA, M.
700 1 $aPEDROSA, F. O.
773 $tJournal of Biotechnology, Amsterdam$gv. 97, n. 3, p. 243-252, Aug. 2002.
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