Data/hora: 17/06/2024 02:19:18

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 30/08/2018
Data da última atualização: | 30/08/2018
Autoria: | MENDES, H. M. F.; ROCHA JÚNIOR, S. S.; PAZ, C. F. R.; VALADARES, R. C.; RIBEIRO, R. M.; AZEVEDO, D. S. D.; VASCONCELOS, A. C.; FALEIROS, R. R.
Afiliação: | Heloisa M. F. Mendes, Universidade Federal dos Vales do Jequitinhonha e Mucuri - UFVJM; Sérgio S. Rocha Júnior, Programa de Pós-Graduação em Ciência Animal/Universidade Federal de Minas Gerais - UFMG; Cahuê F. R. Paz, Programa de Pós-Graduação em Ciência Animal/Universidade Federal de Minas Gerais - UFMG; Rodrigo C. Valadares, Universidade Federal dos Vales do Jequitinhonha e Mucuri - UFVJM; Rodrigo M. Ribeiro, Programa de Pós-Graduação em Ciência Animal/Universidade Federal de Minas Gerais - UFMG; David S. D. Azevedo, Medicina Veterinária/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Anilton C. Vasconcelos, Instituto de Ciências Biológicas/Universidade Federal de Minas Gerais - UFMG; Rafael R. Faleiros, Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG.
Título: | Testing a new surgical instrument to obtain bovine hoof biopsy samples.
Ano de publicação: | 2018
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 6, p. 1077-1084, junho 2018
Idioma: | Inglês
Notas: | Título em português: Teste de viabilidade de um instrumento cirúrgico para obtenção de biópsias do tecido laminar do casco de bovinos.
Conteúdo: | The histological and molecular analysis of biopsy samples are fundamental steps for the understanding of physiopathology, diagnosis and prognosis of the diseases. However, harvest of tissue biopsies from hoof lamellar tissue is a procedure with limitations due to lack of effective surgical instruments and techniques. The aim of the current study is to develop and test in vivo a surgical instrument with the specific purpose of harvesting lamellar tissue in cattle. A prototype called Falcão-Faleiros? lamellotome (INPIBR102013018765-8) was designed, produced and tested. After sedation, five adult cattle were restrained in lateral recumbency and locally anesthetized in two digits. The stratum corneum was worn down using a rotary tool coupled to a 3/8? high-speed cutter until the soft tissue proximity was reached. Next, the inner edge of the worn area was bounded with a scalpel. The lamellotome was introduced to obtain and hold the sample. The histological specimens of 16mm length by 6mm depth were stained with HE, PAS, Masson?s thricome and Shorr. The structures of interest were differentiated in the histological analysis without technical artifacts and a mean number of 85 epidermal laminae per sample were counted. No relevant lameness or wound complication were seen following the procedure. In conclusion the technique using the lamellotme was effective in obtaining lamellar tissue biopsy samples without causing clinical harm in cattle. The procedure showed potential to be used in clinical research or even as a supplementary diagnosis method for routine bovine podiatry.
Palavras-Chave: | Bovine.
Thesagro: | Biopsia; Bovino; Casco; Cirurgia.
Thesaurus Nal: | Biopsy; Cattle; Claws; Hooves; Laminitis.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/182202/1/Testing-a-new-surgical-instrument-to-obtain-bovine.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (62985-1) - UPE - AP - DD -  -  -  - DIS