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Registro Completo |
Biblioteca(s): |
Embrapa Rondônia. |
Data corrente: |
14/01/2016 |
Data da última atualização: |
14/01/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SANTOS, M. R. A. dos; CHAGAS, S. E. da S.; GUIMARÃES, M. de C. M. |
Afiliação: |
MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO; Sâmela Emanuela da Silva Chagas, Faculdade São Lucas; Milene de Castro Melo Guimarães, Mestranda, Unir. |
Título: |
Establishment of protocol for decontamination of leaf explants of Platonia insignis Mart. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Saber Científico, Porto Velho, v. 4, n. 2, p. 15-23, 2015. |
Idioma: |
Inglês |
Conteúdo: |
The objective of this work was the establishment of an efficient protocol for surface sterilization of leaf explants aiming at its introduction in callogenesis and somatic embryogenesis studies. The leaves were washed with distilled water and a detergent agent. In a laminar flow hood they were immersed in alcohol 70% (v/v) for 1 minute and then immersed in calcium hypochlorite solutions 0.25, 0.5, 0.75, 1.0, 1.25, 2.5, 5.0 or 10.0% (w/v) during 30 minutes, rinsed three times in distilled water, and cut in 1cm2 fragments, which were inoculated in MS medium. Half of the explants were inoculated with the abaxial epidermis in contact with the medium and half with the adaxial epidermis facedown. Each experimental unit was represented by 10 explants. The cultures were kept in the dark at 24±2ºC. Seven days after inoculation the percentages of contamination and necrosis of the explants were evaluated. The most efficient were the concentrations of 0.25, 0.5 and 0.75% calcium hypochlorite, which resulted in total sterilization of the explants. Treatments with 1.0, 1.25, 2.5, 5.0 and 10.0% calcium hypochlorite resulted in high level of necrosis (100%), independent of the side in contact with the medium. All the abaxial side facedown explants kept green and the adaxial ones became necrotic. It is recommendable 0.25% calcium hypochlorite in the surface sterilization and the abaxial epidermis in contact with the culture medium. |
Palavras-Chave: |
Cultura de tecidos vegetais; Descontaminação de explantes; Estabelecimento in vitro; Explant decontamination; Hipoclorito de cálcio; In vitro establishment; Plant tissue culture. |
Thesagro: |
Platonia Insignis. |
Thesaurus Nal: |
calcium hypochlorite. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/137016/1/Platonia.pdf
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Marc: |
LEADER 02276naa a2200253 a 4500 001 2033914 005 2016-01-14 008 2015 bl uuuu u00u1 u #d 100 1 $aSANTOS, M. R. A. dos 245 $aEstablishment of protocol for decontamination of leaf explants of Platonia insignis Mart.$h[electronic resource] 260 $c2015 520 $aThe objective of this work was the establishment of an efficient protocol for surface sterilization of leaf explants aiming at its introduction in callogenesis and somatic embryogenesis studies. The leaves were washed with distilled water and a detergent agent. In a laminar flow hood they were immersed in alcohol 70% (v/v) for 1 minute and then immersed in calcium hypochlorite solutions 0.25, 0.5, 0.75, 1.0, 1.25, 2.5, 5.0 or 10.0% (w/v) during 30 minutes, rinsed three times in distilled water, and cut in 1cm2 fragments, which were inoculated in MS medium. Half of the explants were inoculated with the abaxial epidermis in contact with the medium and half with the adaxial epidermis facedown. Each experimental unit was represented by 10 explants. The cultures were kept in the dark at 24±2ºC. Seven days after inoculation the percentages of contamination and necrosis of the explants were evaluated. The most efficient were the concentrations of 0.25, 0.5 and 0.75% calcium hypochlorite, which resulted in total sterilization of the explants. Treatments with 1.0, 1.25, 2.5, 5.0 and 10.0% calcium hypochlorite resulted in high level of necrosis (100%), independent of the side in contact with the medium. All the abaxial side facedown explants kept green and the adaxial ones became necrotic. It is recommendable 0.25% calcium hypochlorite in the surface sterilization and the abaxial epidermis in contact with the culture medium. 650 $acalcium hypochlorite 650 $aPlatonia Insignis 653 $aCultura de tecidos vegetais 653 $aDescontaminação de explantes 653 $aEstabelecimento in vitro 653 $aExplant decontamination 653 $aHipoclorito de cálcio 653 $aIn vitro establishment 653 $aPlant tissue culture 700 1 $aCHAGAS, S. E. da S. 700 1 $aGUIMARÃES, M. de C. M. 773 $tSaber Científico, Porto Velho$gv. 4, n. 2, p. 15-23, 2015.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
22/05/2015 |
Data da última atualização: |
22/05/2015 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
ALVES, G. L.; BISCOLA, P. H. N.; PAULA, N. Q. de. |
Afiliação: |
GABRIEL LOPES ALVES, Graduando da Universidade Federal de Mato Grosso do Sul - UFMS; PAULO HENRIQUE NOGUEIRA BISCOLA, CNPGC; NIBIA QUEIROZ DE PAULA, CNPGC. |
Título: |
A estratégia e a gestão de portfólio de projetos: diagnóstico e sugestão de práticas para a Embrapa Gado de Corte. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 10., 2014, Campo Grande, MS. [Anais da..]. Campo Grande, MS: Embrapa Gado de Corte, 2014. p. 42-43. |
Páginas: |
2 p. |
Série: |
(Embrapa Gado de Corte. Documentos, 208). |
Idioma: |
Português |
Notas: |
Comissão organizadora: Grácia Maria Soares Rosinha, Alexandra Rocha de Oliveir, Rodrigo Carvalho Alva. |
Palavras-Chave: |
Carteira de projetos; Portfólio de Projetos. |
Thesagro: |
Gestão. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/124398/1/DOC208-Final-A-estrategia-e-a-gestao.pdf
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Marc: |
LEADER 00846nam a2200193 a 4500 001 2016035 005 2015-05-22 008 2014 bl uuuu u00u1 u #d 100 1 $aALVES, G. L. 245 $aA estratégia e a gestão de portfólio de projetos$bdiagnóstico e sugestão de práticas para a Embrapa Gado de Corte.$h[electronic resource] 260 $aIn: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 10., 2014, Campo Grande, MS. [Anais da..]. Campo Grande, MS: Embrapa Gado de Corte, 2014. p. 42-43.$c2014 300 $a2 p. 490 $a(Embrapa Gado de Corte. Documentos, 208). 500 $aComissão organizadora: Grácia Maria Soares Rosinha, Alexandra Rocha de Oliveir, Rodrigo Carvalho Alva. 650 $aGestão 653 $aCarteira de projetos 653 $aPortfólio de Projetos 700 1 $aBISCOLA, P. H. N. 700 1 $aPAULA, N. Q. de
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Embrapa Gado de Corte (CNPGC) |
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