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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
19/11/2003 |
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Data da última atualização: |
24/06/2025 |
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Autoria: |
MITCHELL, S. E.; ROBINSON, J. J.; KING, M. E.; McKELVEY, W. A. C.; WILLIAMS, L. M. |
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Título: |
Interleukin 8 in the cervix of non-pregnant ewes. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Reproduction, v. 124, n. 3, p. 409-416, Sep. 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Studies of cervical artificial insemination of ewes at hormone-synchronized oestrus indicate that the cervix remains relatively impenetrable to semen, in contrast to naturally breeding animals. During parturition the inflammatory response plays an important part in cervical dilation and possibly, to a lesser extent, in the non-pregnant cervix at oestrus to facilitate the transcervical transport of semen. The expression of interleukin 8 (IL-8), a pro-inflammatory cytokine in the ovine cervix, has been mapped and quantified, using semi-quantitative in situ hybridization, to ascertain the role played by inflammation in the ovine cervix during natural and artificially induced oestrous cycles. IL-8 gene expression was observed in both the luminal epithelium and fibroblastic cells of the cervix. The presence of IL-8 was confirmed using immunohistochemistry. IL-8 gene expression in the luminal epithelium varied throughout the oestrous cycle and was highest at oestrus and at day 5 of the oestrous cycle. In ewes artificially induced to ovulate, either by the withdrawal of progesterone pessaries after treatment for 12 days, or by two i.m. injections of prostaglandin 9 days apart, IL-8 gene expression at oestrus was significantly lower than it was at natural oestrus. Insemination increased IL-8 gene expression in progesterone-synchronized ewes. These data support the hypothesis that IL-8-induced inflammation is important in normal cervical function and that this process is inhibited during artificial synchronization of the oestrous cycle and is increased by exposure to semen. MenosAbstract: Studies of cervical artificial insemination of ewes at hormone-synchronized oestrus indicate that the cervix remains relatively impenetrable to semen, in contrast to naturally breeding animals. During parturition the inflammatory response plays an important part in cervical dilation and possibly, to a lesser extent, in the non-pregnant cervix at oestrus to facilitate the transcervical transport of semen. The expression of interleukin 8 (IL-8), a pro-inflammatory cytokine in the ovine cervix, has been mapped and quantified, using semi-quantitative in situ hybridization, to ascertain the role played by inflammation in the ovine cervix during natural and artificially induced oestrous cycles. IL-8 gene expression was observed in both the luminal epithelium and fibroblastic cells of the cervix. The presence of IL-8 was confirmed using immunohistochemistry. IL-8 gene expression in the luminal epithelium varied throughout the oestrous cycle and was highest at oestrus and at day 5 of the oestrous cycle. In ewes artificially induced to ovulate, either by the withdrawal of progesterone pessaries after treatment for 12 days, or by two i.m. injections of prostaglandin 9 days apart, IL-8 gene expression at oestrus was significantly lower than it was at natural oestrus. Insemination increased IL-8 gene expression in progesterone-synchronized ewes. These data support the hypothesis that IL-8-induced inflammation is important in normal cervical function and that this process is in... Mostrar Tudo |
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Palavras-Chave: |
AI; Luprostenol. |
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Thesaurus Nal: |
Artificial insemination; Cervix; Estrus synchronization; In situ hybridization; Interleukin-8; Pharmacology; Progesterone; Prostaglandin-F synthase; Reproduction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02443naa a2200313 a 4500
001 1525605
005 2025-06-24
008 2002 bl uuuu u00u1 u #d
100 1 $aMITCHELL, S. E.
245 $aInterleukin 8 in the cervix of non-pregnant ewes.$h[electronic resource]
260 $c2002
520 $aAbstract: Studies of cervical artificial insemination of ewes at hormone-synchronized oestrus indicate that the cervix remains relatively impenetrable to semen, in contrast to naturally breeding animals. During parturition the inflammatory response plays an important part in cervical dilation and possibly, to a lesser extent, in the non-pregnant cervix at oestrus to facilitate the transcervical transport of semen. The expression of interleukin 8 (IL-8), a pro-inflammatory cytokine in the ovine cervix, has been mapped and quantified, using semi-quantitative in situ hybridization, to ascertain the role played by inflammation in the ovine cervix during natural and artificially induced oestrous cycles. IL-8 gene expression was observed in both the luminal epithelium and fibroblastic cells of the cervix. The presence of IL-8 was confirmed using immunohistochemistry. IL-8 gene expression in the luminal epithelium varied throughout the oestrous cycle and was highest at oestrus and at day 5 of the oestrous cycle. In ewes artificially induced to ovulate, either by the withdrawal of progesterone pessaries after treatment for 12 days, or by two i.m. injections of prostaglandin 9 days apart, IL-8 gene expression at oestrus was significantly lower than it was at natural oestrus. Insemination increased IL-8 gene expression in progesterone-synchronized ewes. These data support the hypothesis that IL-8-induced inflammation is important in normal cervical function and that this process is inhibited during artificial synchronization of the oestrous cycle and is increased by exposure to semen.
650 $aArtificial insemination
650 $aCervix
650 $aEstrus synchronization
650 $aIn situ hybridization
650 $aInterleukin-8
650 $aPharmacology
650 $aProgesterone
650 $aProstaglandin-F synthase
650 $aReproduction
650 $aSheep
653 $aAI
653 $aLuprostenol
700 1 $aROBINSON, J. J.
700 1 $aKING, M. E.
700 1 $aMcKELVEY, W. A. C.
700 1 $aWILLIAMS, L. M.
773 $tReproduction$gv. 124, n. 3, p. 409-416, Sep. 2002.
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Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 7 | |
| 1. |  | FIGUEREDO, P. E.; HOOGERHEIDE, E. S. S.; TIAGO, A. V.; ROSSI, A. A. B.; PAULA, M. F. B. de; PINTO, J. M. A.; SILVA, A. P. M. da. Caracterização molecular de etnovariedades de mandioca obtidas da região periurbana de Sinop, Mato Grosso. In: ENCONTRO DE CIÊNCIA E TECNOLOGIAS AGROSSUSTENTÁVEIS, 2.; JORNADA CIENTÍFICA DA EMBRAPA AGROSSILVIPASTORIL, 7., 2018. Sinop, MT. Resumos... Sinop, MT: Embrapa Agrossilpastoril, 2018. p. 130-133.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 2. | | LANES, E. C. M.; VIANA, J. M. S.; PAES, G. P.; PAULA, M. F. B.; MAIA, C.; CAIXETA, E. T.; MIRANDA, G. V. Population structure and genetic diversity of maize inbreds derived from tropical hybrids. Genetics and Molecular Research, v. 13, n. 3, p. 7365-7376, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Café. |
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| 3. | | NASCIMENTO, L. S. do; NEVES, C. G.; AMARAL, D. O. J. do; PAULA, M. F. B. de; COSTA, C. L. dos S.; PASSOS, O. S.; GESTEIRA, A. da S.; MICHELI, F. Avaliação morfológica e agronômica de variedades de tangerinas. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 10., 2016: Cruz das Almas, BA. Traduzindo ciência para o mundo : resumos. Brasília, DF : Embrapa, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 4. | | NASCIMENTO, L. S. do; NEVES, C. G.; SOARES, L. K. D.; AMARAL, D. O. J. do; PAULA, M. F. B. de; COSTA, C. L. dos S.; SOARES FILHO, W. dos S.; PASSOS, O. S.; GESTEIRA, A. da S.; MICHELI, F. Caracterização físico-química de citros para avaliação da qualidade do fruto. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 8., 2014, Cruz das Almas, Ba. Pesquisa: despertando mentes para a inovação e transformando o futuro: [anais]. Cruz das Almas, BA, Embrapa Mandioca e Fruticultura, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 5. | | NEVES, C. G.; AMARAL, D. O. J. do; PAULA, M. F. B. de; NASCIMENTO, L. S. de; COSTANTINO, G.; PASSOS, O. S.; SANTOS, M. do A.; OLLITRAULT, P.; GESTEIRA, A. da S.; LURO, F.; MICHELI, F. Characterization of tropical mandarin collection: Implications for breeding related to fruit quality. Scientia Horticulturae, n.239, 289-299, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 6. | | AMARAL, M. do; PAULA, M. F. B. de; OLLITRAULT, F.; RIVALLAN, R.; SILVA, E. M. de A.; GESTEIRA, A. da S.; LURO, F.; GARCIA, D.; OLLITRAULT, P.; MICHELI, F. Phylogenetic origin of primary and secondary metabolic pathway genes revealed by c. maxima and c. reticulata diagnostic SNPs. Frontiers in Plant Science, v. 24, Sep, 2019. DOI: 10.3389/fpls.2019.01128. eCollection 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 7. | | SANTOS, L. F. dos; SILVA, R. J. S.; AMARAL, D. O. J. do; PAULA, M. F. B. de; FALCÃO, L. L.; LEGAVRE, T.; ALVES, R. M.; MARCELLINO, L. H.; MICHELI, F. Selection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum. Plos One, v. 11, n. 8, e0160646, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 7 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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