Data/hora: 05/06/2024 01:28:02

Biblioteca(s): 	Embrapa Arroz e Feijão.	
Data corrente: 	07/11/2022	
Data da última atualização: 	07/11/2022	
Autoria: 	MATOS, M. K. da S.; BENKO-ISEPPON, A. M.; BEZERRA-NETO, J. P.; FERREIRA-NETO, J. R. C.; WANG, Y.; LIU, H.; PANDOLFI, V.; AMORIM, L. L. B.; WILLADINO, L.; AMORIM, T. C. do V.; KIDO, E. A.; VIANELLO, R. P.; TIMKO, M. P.; BRASILEIRO-VIDAL, A. C.	
Afiliação: 	MITALLE KAREN DA SILVA MATOS, UNIVERSIDADE FEDERAL DE PERNAMBUCO; ANA MARIA BENKO-ISEPPON, UNIVERSIDADE FEDERAL DE PERNAMBUCO; JOAO PACIFICO BEZERRA-NETO, UNIVERSIDADE FEDERAL DE PERNAMBUCO; JOSE RIBAMAR COSTA FERREIRA-NETO, UNIVERSIDADE FEDERAL DE PERNAMBUCO; YU WANG, UNIVERSITY OF VIRGINA; HAI LIU, UNIVERSITY OF VIRGINIA; VALESCA PANDOLFI, UNIVERSIDADE FEDERAL DE PERNAMBUCO; LIDIANE LINDINALVA BARBOSA AMORIM, UNIVERSIDADE FEDERAL DE PERNAMBUCO; LILIA WILLADINO, UNIVERSIDADE FEDERAL DE PERNAMBUCO; THIALISSON CAACI DO VALE AMORIM, UNIVERSIDADE FEDERAL DE PERNAMBUCO; EDERSON AKIO KIDO, UNIVERSIDADE FEDERAL DE PERNAMBUCO; ROSANA PEREIRA VIANELLO, CNPAF; MICHAEL P. TIMKO, UNIVERSITY OF VIRGINIA; ANA CHRISTINA BRASILEIRO-VIDAL, UNIVERSIDADE FEDERAL DE PERNAMBUCO.	
Título: 	The WRKY transcription factor family in cowpea: Genomic characterization and transcriptomic profiling under root dehydration.	
Ano de publicação: 	2022	
Fonte/Imprenta: 	Gene, v. 823, 146377, May 2022.	
ISSN: 	0378-1119	
DOI: 	https://doi.org/10.1016/j.gene.2022.146377	
Idioma: 	Inglês	
Conteúdo: 	Cowpea [Vigna unguiculata (L.) Walp.] is one of the most tolerant legume crops to drought and salt stresses. WRKY transcription factor (TF) family members stand out among plant transcriptional regulators related to abiotic stress tolerance. However, little information is currently available on the expression of the cowpea WRKY gene family (VuWRKY) in response to water deficit. Thus, we analyzed genomic and transcriptomic data from cowpea to identify VuWRKY members and characterize their structure and transcriptional response under root dehydration stress. Ninety-two complete VuWRKY genes were found in the cowpea genome based on their domain characteristics. They were clustered into three groups: I (15 members), II (58), and III (16), while three genes were unclassified. Domain analysis of the encoded proteins identified four major variants of the conserved heptapeptide motif WRKYGQK. In silico analysis of VuWRKY gene promoters identified eight candidate binding motifs of cis-regulatory elements, regulated mainly by six TF families associated with abiotic stress responses. Ninety-seven VuWRKY modulated splicing variants associated with 55 VuWRKY genes were identified via RNA-Seq analysis available at the Cowpea Genomics Consortium (CpGC) database. qPCR analyses showed that 22 genes are induced under root dehydration, with VuWRKY18, 21, and 75 exhibiting the most significant induction levels. Given their central role in activating signal transduction cascades in abiotic stress response, the data provide a foundation for the targeted modification of specific VuWRKY family members to improve drought tolerance in this important climate-resilient legume in the developing world and beyond.	
Palavras-Chave: 	QPCR; RNA-Seq.	
Thesagro: 	Seca; Vigna Unguiculata.	
Thesaurus Nal: 	Abiotic stress; Cowpeas; Drought tolerance; Gene expression.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Arroz e Feijão (CNPAF)	
Exemplares: 	CNPAF - (36544-1) - UPC - AP - DD - 2022 -  - 2022 - DIS