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Registro Completo |
Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
13/11/2018 |
Data da última atualização: |
03/12/2018 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
SANTOS, S. C. dos; AMORIM NETO, M. F. de; XAVIER, M. E. V.; NORONHA, M. de A.; COSTA, J. G. da. |
Afiliação: |
SANIEL CARLOS DOS SANTOS; MANOEL FERREIRA DE AMORIM NETO; MARIA EUGENIA VIEIRA XAVIER; MARISSONIA DE ARAUJO NORONHA, CPATC; JOAO GOMES DA COSTA, CPATC. |
Título: |
Coleta e caracterização morfológica de acessos de inhame provenientes dos municípios produtores de Alagoas e Sergipe. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA TABULEIROS COSTEIROS, 8., 2018, Aracaju. Anais... Aracaju: Embrapa Tabuleiros Costeiros, 2018. Editor técnico: Ronaldo Souza Resende. |
Páginas: |
p. 70-72. |
Idioma: |
Português |
Thesagro: |
Inhame; Produção. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/185905/1/Coleta.pdf
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Marc: |
LEADER 00759nam a2200181 a 4500 001 2099261 005 2018-12-03 008 2018 bl uuuu u00u1 u #d 100 1 $aSANTOS, S. C. dos 245 $aColeta e caracterização morfológica de acessos de inhame provenientes dos municípios produtores de Alagoas e Sergipe.$h[electronic resource] 260 $aIn: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA TABULEIROS COSTEIROS, 8., 2018, Aracaju. Anais... Aracaju: Embrapa Tabuleiros Costeiros, 2018. Editor técnico: Ronaldo Souza Resende.$c2018 300 $ap. 70-72. 650 $aInhame 650 $aProdução 700 1 $aAMORIM NETO, M. F. de 700 1 $aXAVIER, M. E. V. 700 1 $aNORONHA, M. de A. 700 1 $aCOSTA, J. G. da
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Embrapa Tabuleiros Costeiros (CPATC) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
23/01/2006 |
Data da última atualização: |
29/05/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MOTA, F. F. da; GOMES, E. A.; PAIVA, E.; SELDIN, L. |
Afiliação: |
ELIANE APARECIDA GOMES, CNPMS; EMBRAPA-CNPMS. |
Título: |
Assessment of the diversity of Paenibacillus species in environmental samples by a novel rpoB-based PCR-DGGE method. |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
FEMS Microbiology Ecology, Amsterdam, v. 53, n. 2, p. 317-328, 2005. |
Idioma: |
Inglês |
Conteúdo: |
A specific PCR system based on the gene encoding the RNA polymerase beta subunit, rpoB, was developed for amplification and denaturing gradient gel electrophoresis (DGGE) fingerprinting of Paenibacillus communities in environmental samples. This gene has been previously proven to be a powerful identification tool for the discrimination of species within the genus Paenibacillus and could avoid the limitations of 16S rRNA-based phylogenetic analysis. Initially, the PCR system based on universal rpoB primers were used to amplify DNAs of different Paenibacillus species. A new reverse primer (rpoBPAEN) was further designed based on an insertion of six nucleotides in the Paenibacillus sequences analyzed. This semi-nested PCR system was evaluated for specificity using DNAs isolated from 27 Paenibacillus species belonging to different 16S rRNA-based phylogenetic groups and seven non-Paenibacillus species. The non-Paenibacillus species were not amplified using this PCR approach and one group of Paenibacillus species consisting of strains without the six-base insert also were not amplified; these latter strains were found to be distinct based on 16S rRNA gene phylogeny. In addition, a clone library was generated from the rpoB fragments amplified from two Brazilian soil types (Cerrado and Forest) and all 62 clones sequenced were closely related to one of the 22 sequences from Paenibacillus previously obtained in this study. To assess the diversity of Paenibacillus species in Cerrado and Forest soils and in the rhizosphere of different cultivars of maize, a PCR-DGGE system was used. The Paenibacillus DGGE fingerprints showed a clear distinction between communities of Paenibacillus in Forest and Cerrado soils and rhizosphere samples clustered along Cerrado soil. Profiles of cultivars CMS22 and CMS36 clustered together, with only 53% of similarity to CMS11 and CMS04. The results presented here demonstrate the potential use of the rpoB-based Paenibacillus-specific PCR-DGGE method for studying the diversity of Paenibacillus populations in natural environments. MenosA specific PCR system based on the gene encoding the RNA polymerase beta subunit, rpoB, was developed for amplification and denaturing gradient gel electrophoresis (DGGE) fingerprinting of Paenibacillus communities in environmental samples. This gene has been previously proven to be a powerful identification tool for the discrimination of species within the genus Paenibacillus and could avoid the limitations of 16S rRNA-based phylogenetic analysis. Initially, the PCR system based on universal rpoB primers were used to amplify DNAs of different Paenibacillus species. A new reverse primer (rpoBPAEN) was further designed based on an insertion of six nucleotides in the Paenibacillus sequences analyzed. This semi-nested PCR system was evaluated for specificity using DNAs isolated from 27 Paenibacillus species belonging to different 16S rRNA-based phylogenetic groups and seven non-Paenibacillus species. The non-Paenibacillus species were not amplified using this PCR approach and one group of Paenibacillus species consisting of strains without the six-base insert also were not amplified; these latter strains were found to be distinct based on 16S rRNA gene phylogeny. In addition, a clone library was generated from the rpoB fragments amplified from two Brazilian soil types (Cerrado and Forest) and all 62 clones sequenced were closely related to one of the 22 sequences from Paenibacillus previously obtained in this study. To assess the diversity of Paenibacillus species in Cerrado an... Mostrar Tudo |
Palavras-Chave: |
Cerrado soil; Diversity; Forest soil; Maize rhizosphere; PCR-DGGE; RpoB. |
Thesaurus NAL: |
Paenibacillus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02787naa a2200241 a 4500 001 1489140 005 2018-05-29 008 2005 bl uuuu u00u1 u #d 100 1 $aMOTA, F. F. da 245 $aAssessment of the diversity of Paenibacillus species in environmental samples by a novel rpoB-based PCR-DGGE method.$h[electronic resource] 260 $c2005 520 $aA specific PCR system based on the gene encoding the RNA polymerase beta subunit, rpoB, was developed for amplification and denaturing gradient gel electrophoresis (DGGE) fingerprinting of Paenibacillus communities in environmental samples. This gene has been previously proven to be a powerful identification tool for the discrimination of species within the genus Paenibacillus and could avoid the limitations of 16S rRNA-based phylogenetic analysis. Initially, the PCR system based on universal rpoB primers were used to amplify DNAs of different Paenibacillus species. A new reverse primer (rpoBPAEN) was further designed based on an insertion of six nucleotides in the Paenibacillus sequences analyzed. This semi-nested PCR system was evaluated for specificity using DNAs isolated from 27 Paenibacillus species belonging to different 16S rRNA-based phylogenetic groups and seven non-Paenibacillus species. The non-Paenibacillus species were not amplified using this PCR approach and one group of Paenibacillus species consisting of strains without the six-base insert also were not amplified; these latter strains were found to be distinct based on 16S rRNA gene phylogeny. In addition, a clone library was generated from the rpoB fragments amplified from two Brazilian soil types (Cerrado and Forest) and all 62 clones sequenced were closely related to one of the 22 sequences from Paenibacillus previously obtained in this study. To assess the diversity of Paenibacillus species in Cerrado and Forest soils and in the rhizosphere of different cultivars of maize, a PCR-DGGE system was used. The Paenibacillus DGGE fingerprints showed a clear distinction between communities of Paenibacillus in Forest and Cerrado soils and rhizosphere samples clustered along Cerrado soil. Profiles of cultivars CMS22 and CMS36 clustered together, with only 53% of similarity to CMS11 and CMS04. The results presented here demonstrate the potential use of the rpoB-based Paenibacillus-specific PCR-DGGE method for studying the diversity of Paenibacillus populations in natural environments. 650 $aPaenibacillus 653 $aCerrado soil 653 $aDiversity 653 $aForest soil 653 $aMaize rhizosphere 653 $aPCR-DGGE 653 $aRpoB 700 1 $aGOMES, E. A. 700 1 $aPAIVA, E. 700 1 $aSELDIN, L. 773 $tFEMS Microbiology Ecology, Amsterdam$gv. 53, n. 2, p. 317-328, 2005.
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