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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
26/06/2000 |
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Data da última atualização: |
09/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BARDUCHE, D.; PAIVA, R.; LOPES, M. A.; PAIVA, E. |
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Afiliação: |
EMBRAPA-CNPMS. |
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Título: |
Effect of ABA and GA3 on protein mobilization in embryos and Cotyledons of angico (Anadenanthera peregrina (L.) sepg) seeds during germination. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Brazilian Archives of Biology and Technology, Curitiba, v. 42, n. 2, p. 135-144, 1999. |
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Idioma: |
Inglês |
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Conteúdo: |
In this work, a woody species [A. peregrina (L.) Speg.] was studied in order to observe the effect of ABA and GA3 at the biochemical level during the process of seed germination. Embryos incubated in sucrose solution containing ABA and/or GA3 were analyzed through SDS-PAGE to observe the mobilization pattern of storage proteins during the beginning of germination. Cotyledons isolated from seeds incubated in aqueous solutions containing ABA and/or GA3, were also analyzed through SDS-PAGE and by PAGE/Activity Gels (polyacrylamide gels copolymerized with substrate for enzymes) to observe the mobilization pattern of storage proteins and protease activity after the beginning of the germination. Results of these experiments show that ABA blocks protein mobilization by inhibiting protease activity in cotyledons. This inhibition is not sufficient to prevent germination showing that the effect of ABA on germination is not dependent on protease activity. The blockage of storage protein mobilization was also observed in embryos, but no protease activity inhibition was clearly detected. ABA was able to induce the synthesis of proteins in cotyledons but not in embryos. A polypeptide with an approximate molecular weight of 17 kD, was degraded within 6 hours in control embryos, but this degradation was blocked by ABA and GA3. Using the same concentrations of ABA and GA3 on embryos and cotyledons, the effect of ABA was counteracted by GA3 in embryos, but not in cotyledons. Although the effects of ABA and GA3 were not so different from those shown in the literature, the behavior of 17 kD-polypeptide contradicts these reports suggesting that specific studies should be performed. MenosIn this work, a woody species [A. peregrina (L.) Speg.] was studied in order to observe the effect of ABA and GA3 at the biochemical level during the process of seed germination. Embryos incubated in sucrose solution containing ABA and/or GA3 were analyzed through SDS-PAGE to observe the mobilization pattern of storage proteins during the beginning of germination. Cotyledons isolated from seeds incubated in aqueous solutions containing ABA and/or GA3, were also analyzed through SDS-PAGE and by PAGE/Activity Gels (polyacrylamide gels copolymerized with substrate for enzymes) to observe the mobilization pattern of storage proteins and protease activity after the beginning of the germination. Results of these experiments show that ABA blocks protein mobilization by inhibiting protease activity in cotyledons. This inhibition is not sufficient to prevent germination showing that the effect of ABA on germination is not dependent on protease activity. The blockage of storage protein mobilization was also observed in embryos, but no protease activity inhibition was clearly detected. ABA was able to induce the synthesis of proteins in cotyledons but not in embryos. A polypeptide with an approximate molecular weight of 17 kD, was degraded within 6 hours in control embryos, but this degradation was blocked by ABA and GA3. Using the same concentrations of ABA and GA3 on embryos and cotyledons, the effect of ABA was counteracted by GA3 in embryos, but not in cotyledons. Although the effe... Mostrar Tudo |
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Palavras-Chave: |
ABA; GA3; Mobilizacao; Mobilization; Protease activity; Protein; Tropical woody plant. |
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Thesagro: |
Germinação; Proteína. |
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Thesaurus NAL: |
germination. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/48211/1/Effect-ABA.pdf
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Marc: |
LEADER 02520naa a2200277 a 4500
001 1484074
005 2018-06-09
008 1999 bl uuuu u00u1 u #d
100 1 $aBARDUCHE, D.
245 $aEffect of ABA and GA3 on protein mobilization in embryos and Cotyledons of angico (Anadenanthera peregrina (L.) sepg) seeds during germination.$h[electronic resource]
260 $c1999
520 $aIn this work, a woody species [A. peregrina (L.) Speg.] was studied in order to observe the effect of ABA and GA3 at the biochemical level during the process of seed germination. Embryos incubated in sucrose solution containing ABA and/or GA3 were analyzed through SDS-PAGE to observe the mobilization pattern of storage proteins during the beginning of germination. Cotyledons isolated from seeds incubated in aqueous solutions containing ABA and/or GA3, were also analyzed through SDS-PAGE and by PAGE/Activity Gels (polyacrylamide gels copolymerized with substrate for enzymes) to observe the mobilization pattern of storage proteins and protease activity after the beginning of the germination. Results of these experiments show that ABA blocks protein mobilization by inhibiting protease activity in cotyledons. This inhibition is not sufficient to prevent germination showing that the effect of ABA on germination is not dependent on protease activity. The blockage of storage protein mobilization was also observed in embryos, but no protease activity inhibition was clearly detected. ABA was able to induce the synthesis of proteins in cotyledons but not in embryos. A polypeptide with an approximate molecular weight of 17 kD, was degraded within 6 hours in control embryos, but this degradation was blocked by ABA and GA3. Using the same concentrations of ABA and GA3 on embryos and cotyledons, the effect of ABA was counteracted by GA3 in embryos, but not in cotyledons. Although the effects of ABA and GA3 were not so different from those shown in the literature, the behavior of 17 kD-polypeptide contradicts these reports suggesting that specific studies should be performed.
650 $agermination
650 $aGerminação
650 $aProteína
653 $aABA
653 $aGA3
653 $aMobilizacao
653 $aMobilization
653 $aProtease activity
653 $aProtein
653 $aTropical woody plant
700 1 $aPAIVA, R.
700 1 $aLOPES, M. A.
700 1 $aPAIVA, E.
773 $tBrazilian Archives of Biology and Technology, Curitiba$gv. 42, n. 2, p. 135-144, 1999.
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Embrapa Milho e Sorgo (CNPMS) |
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