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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
18/11/2013 |
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Data da última atualização: |
21/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PAES, R. de C. da S.; FONSECA JUNIOR, A. A.; MONTEIRO, L. A. R. C.; JARDIM, G. C.; PIOVEZAN, U.; HERRERA, H. M.; MAURO, R. A.; VIEIRA-da-MOTTA, O. |
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Afiliação: |
RITA DE CÁSSIA DA SILVA PAES; UBIRATAN PIOVEZAN, CPAP. |
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Título: |
Serological and molecular investigation of the prevalence of Aujeszky's disease in feral swine (Sus scrofa) in the subregions of the Pantanal wetland, Brazil. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Veterinary Microbiology, v. 165, p. 448-454, 2013. |
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ISSN: |
0378-1135 |
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Idioma: |
Inglês |
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Conteúdo: |
The feral swine (FS) originated from the domestic pig and is present throughout the Brazilian wetland plain (the Pantanal). Aujeszky?s disease (AD) was first serologically confirmed in the state of Mato Grosso do Sul (MS) in 2001; however, there was no viral confirmation. The aim of this study was to investigate antibodies against-SuHV-1 in the sera of feral swine in the studied areas, detect SuHV-1 through PCR and classify the viral genome. Among the 218 animals sampled, 186 were analyzed by ELISA, resulting in 88 (47.3%) reactive samples. In the serum neutralization test (SN), 57/179 (31.8%) samples presented antibodies against the AD virus (SuHV-1). By nested PCR, 104 DNA samples were extracted for analysis and confirmed with amplification of a fragment of glycoprotein B (gB) in five samples. The SuHV-1 was detected in 12 samples by using primers for glycoprotein E (gE) and viral genome was classified as Type I by ul44 partial sequencing. The amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows indicate that the vertical transmission contribute to maintain SuHV-1 in a free-living feral swine population. The origin of AD in the feral swine populations of the Pantanal is unknown, however, the determination of viral latency, the vertical transmission of the antigen by the amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows and genome typing contribute to the elucidation of the epidemiology of this disease in the wetlands of MS, Brazil. MenosThe feral swine (FS) originated from the domestic pig and is present throughout the Brazilian wetland plain (the Pantanal). Aujeszky?s disease (AD) was first serologically confirmed in the state of Mato Grosso do Sul (MS) in 2001; however, there was no viral confirmation. The aim of this study was to investigate antibodies against-SuHV-1 in the sera of feral swine in the studied areas, detect SuHV-1 through PCR and classify the viral genome. Among the 218 animals sampled, 186 were analyzed by ELISA, resulting in 88 (47.3%) reactive samples. In the serum neutralization test (SN), 57/179 (31.8%) samples presented antibodies against the AD virus (SuHV-1). By nested PCR, 104 DNA samples were extracted for analysis and confirmed with amplification of a fragment of glycoprotein B (gB) in five samples. The SuHV-1 was detected in 12 samples by using primers for glycoprotein E (gE) and viral genome was classified as Type I by ul44 partial sequencing. The amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows indicate that the vertical transmission contribute to maintain SuHV-1 in a free-living feral swine population. The origin of AD in the feral swine populations of the Pantanal is unknown, however, the determination of viral latency, the vertical transmission of the antigen by the amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows and genome typing contribute to the elucidation of the epidemiology of this disease in the we... Mostrar Tudo |
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Palavras-Chave: |
Feral swine; Glycoprotein; PCR; Seroneutralization assay. |
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Thesagro: |
Elisa. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/92652/1/1-s2.0-S0378113513002009-main.pdf
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Marc: |
LEADER 02356naa a2200277 a 4500
001 1971371
005 2017-07-21
008 2013 bl uuuu u00u1 u #d
022 $a0378-1135
100 1 $aPAES, R. de C. da S.
245 $aSerological and molecular investigation of the prevalence of Aujeszky's disease in feral swine (Sus scrofa) in the subregions of the Pantanal wetland, Brazil.
260 $c2013
520 $aThe feral swine (FS) originated from the domestic pig and is present throughout the Brazilian wetland plain (the Pantanal). Aujeszky?s disease (AD) was first serologically confirmed in the state of Mato Grosso do Sul (MS) in 2001; however, there was no viral confirmation. The aim of this study was to investigate antibodies against-SuHV-1 in the sera of feral swine in the studied areas, detect SuHV-1 through PCR and classify the viral genome. Among the 218 animals sampled, 186 were analyzed by ELISA, resulting in 88 (47.3%) reactive samples. In the serum neutralization test (SN), 57/179 (31.8%) samples presented antibodies against the AD virus (SuHV-1). By nested PCR, 104 DNA samples were extracted for analysis and confirmed with amplification of a fragment of glycoprotein B (gB) in five samples. The SuHV-1 was detected in 12 samples by using primers for glycoprotein E (gE) and viral genome was classified as Type I by ul44 partial sequencing. The amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows indicate that the vertical transmission contribute to maintain SuHV-1 in a free-living feral swine population. The origin of AD in the feral swine populations of the Pantanal is unknown, however, the determination of viral latency, the vertical transmission of the antigen by the amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows and genome typing contribute to the elucidation of the epidemiology of this disease in the wetlands of MS, Brazil.
650 $aElisa
653 $aFeral swine
653 $aGlycoprotein
653 $aPCR
653 $aSeroneutralization assay
700 1 $aFONSECA JUNIOR, A. A.
700 1 $aMONTEIRO, L. A. R. C.
700 1 $aJARDIM, G. C.
700 1 $aPIOVEZAN, U.
700 1 $aHERRERA, H. M.
700 1 $aMAURO, R. A.
700 1 $aVIEIRA-da-MOTTA, O.
773 $tVeterinary Microbiology$gv. 165, p. 448-454, 2013.
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Registro original: |
Embrapa Pantanal (CPAP) |
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| Registros recuperados : 3 | |
| 1. |  | SANTOS, C. A. F.; CAVALCANTI, J.; PAINI, J. N.; CRUZ, C. D. Correlacoes canonicas entre componentes primarios e secundarios da producao de graos em guandu (Cajanus cajan (L.) Millsp.). Revista Ceres, Viçosa, MG, v. 41, n. 236, p. 459-464, 1994.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Semiárido. |
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| 2. | | SANTOS, C. A. F.; MENEZES, E. A.; PAINI, J. N.; CRUZ, C. D. Coeficiente de trilha no estudo dos componentes primarios e secundarios na producao de graos do guandu (Cajanus cajan (L.) Millsp). Revista Ceres, Viçosa, MG, v. 41, n. 235, p. 299-305, 1994.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Semiárido. |
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| 3. | | SANTOS, C. A. F.; MENEZES, E. A.; PAINI, J. N.; CRUZ, C. D. Coeficiente de trilha no estudo dos componentes de produção do guandu (Cajanus cajan (L.) Millap). Revista Brasileira de Genética, Ribeirão Preto, v. 16, n. 3, p. 367, 1993. Suplemento. Edição dos Resumos do 39 Congresso Nacional de Genética, Caxambu, 1993.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 3 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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