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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
29/03/2019 |
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Data da última atualização: |
21/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMARGO, L. S. de A.; MUNK, M.; SALES, J. N.; WOHLRES-VIANA, S.; QUINTAO, C. C. R.; VIANA, J. H. M. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MICHELE MUNK, UFJF; JOSE NELIO SALES, UFLA; SABINE WOHLRES-VIANA, UFJF; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Differential gene expression between in vivo and in vitro maturation: a comparative study with bovine oocytes derived from the same donor pool. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
JBRA Assisted Reproduction, v. 23, n. 1, p. 7-14, 2019. |
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DOI: |
10.5935/1518-0557.20180084 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Luiz Sergio Almeida Camargo. |
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Conteúdo: |
Objective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of polyadenylated transcripts in the oocyte cytoplasm when compared to in vivo maturation induced by exogenous hormones in oocytes collected from the same donor pool. MenosObjective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of p... Mostrar Tudo |
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Palavras-Chave: |
In vivo maturation; MRNA; Ovum pick-up. |
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Thesaurus Nal: |
Gene expression. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/195183/1/jbra-23-01-0007.pdf
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Marc: |
LEADER 02498naa a2200253 a 4500
001 2107759
005 2022-06-21
008 2019 bl uuuu u00u1 u #d
024 7 $a10.5935/1518-0557.20180084$2DOI
100 1 $aCAMARGO, L. S. de A.
245 $aDifferential gene expression between in vivo and in vitro maturation$ba comparative study with bovine oocytes derived from the same donor pool.$h[electronic resource]
260 $c2019
500 $aNa publicação: Luiz Sergio Almeida Camargo.
520 $aObjective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of polyadenylated transcripts in the oocyte cytoplasm when compared to in vivo maturation induced by exogenous hormones in oocytes collected from the same donor pool.
650 $aGene expression
653 $aIn vivo maturation
653 $aMRNA
653 $aOvum pick-up
700 1 $aMUNK, M.
700 1 $aSALES, J. N.
700 1 $aWOHLRES-VIANA, S.
700 1 $aQUINTAO, C. C. R.
700 1 $aVIANA, J. H. M.
773 $tJBRA Assisted Reproduction$gv. 23, n. 1, p. 7-14, 2019.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
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Registro |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
13/07/2021 |
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Data da última atualização: |
13/07/2021 |
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Tipo da produção científica: |
Circular Técnica |
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Autoria: |
SANTANA, F. M.; LAU, D.; SBALCHEIRO, C. C.; FERREIRA, L. J.; GUTERRES, C. W.; PÁDUA, J. M. V. |
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Afiliação: |
FLAVIO MARTINS SANTANA, CNPT; DOUGLAS LAU, CNPT; CHEILA CRISTINA SBALCHEIRO, CNPT; LEANDRO JOSE FERREIRA, SGI; CAROLINE WESP GUTERRES, bióloga, doutora em Fitotecnia, pesquisadora da CCGL Tecnologia, Cruz Alta, RS.; JOSÉ MARIA VILLELA PÁDUA, engenheiro-agrônomo, doutor em Agronomia/Melhoramento de Plantas, professor e pesquisador na Universidade Federal de Lavras, Lavras, MG. |
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Título: |
Eficiência de fungicidas para controle de manchas foliares do trigo: resultados dos Ensaios Cooperativos - Safras 2018 e 2019. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Passo Fundo, RS: Embrapa Trigo, maio 2021. |
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Páginas: |
23 |
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Série: |
1518-6490 |
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ISSN: |
(Embrapa Trigo. Circular Técnica online, 64) |
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Idioma: |
Português |
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Conteúdo: |
As manchas foliares em trigo são doenças de importância econômica global (Kremneva et al, 2020). O complexo de manchas foliares em trigo é causado por um grupo de fungos, sendo os principais patógenos Drechslera triticirepentis (mancha bronzeada ou mancha amarela), Bipolaris sorokiniana (mancha marrom) e Stagonospora nodorum (mancha da gluma). Esses patógenos possuem em comum a alta habilidade saprofítica, os sintomas formam lesões necróticas com halo clorótico e possuem capacidade de sobreviver nas sementes e restos culturais (Fernandes; Picinini, 1999). |
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Palavras-Chave: |
Drechslera triticirepentis. |
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Thesagro: |
Doença de Planta; Doença Fúngica; Fungo; Mancha Foliar; Trigo. |
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Thesaurus NAL: |
Bipolaris sorokiniana. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/224433/1/CircTec-64-o.pdf
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Marc: |
LEADER 01458nam a2200289 a 4500
001 2132928
005 2021-07-13
008 2021 bl uuuu u0uu1 u #d
022 $a(Embrapa Trigo. Circular Técnica online, 64)
100 1 $aSANTANA, F. M.
245 $aEficiência de fungicidas para controle de manchas foliares do trigo$bresultados dos Ensaios Cooperativos - Safras 2018 e 2019.$h[electronic resource]
260 $aPasso Fundo, RS: Embrapa Trigo, maio 2021.$c2021
300 $a23
490 $a1518-6490
520 $aAs manchas foliares em trigo são doenças de importância econômica global (Kremneva et al, 2020). O complexo de manchas foliares em trigo é causado por um grupo de fungos, sendo os principais patógenos Drechslera triticirepentis (mancha bronzeada ou mancha amarela), Bipolaris sorokiniana (mancha marrom) e Stagonospora nodorum (mancha da gluma). Esses patógenos possuem em comum a alta habilidade saprofítica, os sintomas formam lesões necróticas com halo clorótico e possuem capacidade de sobreviver nas sementes e restos culturais (Fernandes; Picinini, 1999).
650 $aBipolaris sorokiniana
650 $aDoença de Planta
650 $aDoença Fúngica
650 $aFungo
650 $aMancha Foliar
650 $aTrigo
653 $aDrechslera triticirepentis
700 1 $aLAU, D.
700 1 $aSBALCHEIRO, C. C.
700 1 $aFERREIRA, L. J.
700 1 $aGUTERRES, C. W.
700 1 $aPÁDUA, J. M. V.
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Registro original: |
Embrapa Trigo (CNPT) |
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