| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
10/02/2012 |
|
Data da última atualização: |
27/02/2023 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
ABREU, E. F. M.; ANDRADE, E. C. de; ARAGAO, F. J. L. |
|
Afiliação: |
Emanuel Felipe Medereiros Abreu, UnB; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; FRANCISCO JOSE LIMA ARAGAO, CENARGEN. |
|
Título: |
Molecular comparison between isolates of Cowpea severe mosaic virus and Cowpea aphid borne mosaic virus from Northeast Brazil. |
|
Ano de publicação: |
2011 |
|
Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. pdf 34699 |
|
Páginas: |
p. 6 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Cowpea (Vigna unguiculata) is an important plant crop in Northeast Brazil being traditionally cultivated by small farmers. Virus diseases are considered to be the main factor limiting cowpea yield in the region. The severe mosaic disease caused by the Cowpea severe mosaic virus (CpSMV), family Comoviridae, genus Comovirus, seems to be one of the most prevalent diseases leading to high yield losses in this crop. The CABMV belongs to the genus Potyvirus in the Potyviridae family, and infects cowpea worldwide. In the northeastern Region of Brazil, both viruses can be found in cowpea planted areas. The aim of the present study was to access the degree of homology among a 2500 and 1500bp region of different isolates of CpSMV and CABMV, respectively; obtained in different northeastern regions in Brazil, and to compare it to isolates throughout the world. Plants with CPSMV and CABMV symptoms from the states of Piauí, Ceará, Rio Grande do Norte, Paraiba, Pernambuco, Alagoas, Sergipe and Bahia were collected, and the isolates were identified by RT-PCR analysis. Total RNA was extracted from infected tissue with Trizol Reagent (Invitrogen) according to manufacturer's recommendations, and afterwards used for synthesis of cDNA fragments by RT-PCR using the Superscript® III Reverse Polymerase and Taq DNA Polymerase (Invitrogen), according to manufacturer?s recommendations. The synthesized primers were able to amplify fragments of 2500 and 1500bp of the CpSMV and CABMV virus, respectively, by RT-PCR. Amplification products were directly cloned into the pGEMT-Easy plasmid vector (Promega), according to the manufacturer?s instructions. Cloned fragments were sequenced in both orientations. Deduced amino acid sequences of the virus were compared to sequences available from GenBank. Multiple sequence alignments were obtained with Clustal W. Phylogenetic trees using the MEGA version 4.1 software package and the neighbour-joining method with Poisson correction. Tree branches were bootstrapped with 1000 permutations. CpSMV and CABMV diseases remain as limiting factors in this crop in Brazil, and breeding programs, either by conventional or engineered approaches, should be targeted at establishing resistance of cowpeas to CpSMV and CABMV. MenosCowpea (Vigna unguiculata) is an important plant crop in Northeast Brazil being traditionally cultivated by small farmers. Virus diseases are considered to be the main factor limiting cowpea yield in the region. The severe mosaic disease caused by the Cowpea severe mosaic virus (CpSMV), family Comoviridae, genus Comovirus, seems to be one of the most prevalent diseases leading to high yield losses in this crop. The CABMV belongs to the genus Potyvirus in the Potyviridae family, and infects cowpea worldwide. In the northeastern Region of Brazil, both viruses can be found in cowpea planted areas. The aim of the present study was to access the degree of homology among a 2500 and 1500bp region of different isolates of CpSMV and CABMV, respectively; obtained in different northeastern regions in Brazil, and to compare it to isolates throughout the world. Plants with CPSMV and CABMV symptoms from the states of Piauí, Ceará, Rio Grande do Norte, Paraiba, Pernambuco, Alagoas, Sergipe and Bahia were collected, and the isolates were identified by RT-PCR analysis. Total RNA was extracted from infected tissue with Trizol Reagent (Invitrogen) according to manufacturer's recommendations, and afterwards used for synthesis of cDNA fragments by RT-PCR using the Superscript® III Reverse Polymerase and Taq DNA Polymerase (Invitrogen), according to manufacturer?s recommendations. The synthesized primers were able to amplify fragments of 2500 and 1500bp of the CpSMV and CABMV virus, respectively,... Mostrar Tudo |
|
Palavras-Chave: |
CABMV; Cowpea; CpSMV; Molecular and RT-PCR analysis; Virus diseases. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 02995nam a2200205 a 4500
001 1915005
005 2023-02-27
008 2011 bl uuuu u01u1 u #d
100 1 $aABREU, E. F. M.
245 $aMolecular comparison between isolates of Cowpea severe mosaic virus and Cowpea aphid borne mosaic virus from Northeast Brazil.$h[electronic resource]
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. pdf 34699$c2011
300 $ap. 6
520 $aCowpea (Vigna unguiculata) is an important plant crop in Northeast Brazil being traditionally cultivated by small farmers. Virus diseases are considered to be the main factor limiting cowpea yield in the region. The severe mosaic disease caused by the Cowpea severe mosaic virus (CpSMV), family Comoviridae, genus Comovirus, seems to be one of the most prevalent diseases leading to high yield losses in this crop. The CABMV belongs to the genus Potyvirus in the Potyviridae family, and infects cowpea worldwide. In the northeastern Region of Brazil, both viruses can be found in cowpea planted areas. The aim of the present study was to access the degree of homology among a 2500 and 1500bp region of different isolates of CpSMV and CABMV, respectively; obtained in different northeastern regions in Brazil, and to compare it to isolates throughout the world. Plants with CPSMV and CABMV symptoms from the states of Piauí, Ceará, Rio Grande do Norte, Paraiba, Pernambuco, Alagoas, Sergipe and Bahia were collected, and the isolates were identified by RT-PCR analysis. Total RNA was extracted from infected tissue with Trizol Reagent (Invitrogen) according to manufacturer's recommendations, and afterwards used for synthesis of cDNA fragments by RT-PCR using the Superscript® III Reverse Polymerase and Taq DNA Polymerase (Invitrogen), according to manufacturer?s recommendations. The synthesized primers were able to amplify fragments of 2500 and 1500bp of the CpSMV and CABMV virus, respectively, by RT-PCR. Amplification products were directly cloned into the pGEMT-Easy plasmid vector (Promega), according to the manufacturer?s instructions. Cloned fragments were sequenced in both orientations. Deduced amino acid sequences of the virus were compared to sequences available from GenBank. Multiple sequence alignments were obtained with Clustal W. Phylogenetic trees using the MEGA version 4.1 software package and the neighbour-joining method with Poisson correction. Tree branches were bootstrapped with 1000 permutations. CpSMV and CABMV diseases remain as limiting factors in this crop in Brazil, and breeding programs, either by conventional or engineered approaches, should be targeted at establishing resistance of cowpeas to CpSMV and CABMV.
653 $aCABMV
653 $aCowpea
653 $aCpSMV
653 $aMolecular and RT-PCR analysis
653 $aVirus diseases
700 1 $aANDRADE, E. C. de
700 1 $aARAGAO, F. J. L.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Meio Ambiente. |
|
Data corrente: |
10/06/2022 |
|
Data da última atualização: |
14/07/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
SILVA, R. B. da; ANTUNES, T.; ROSA, J. S.; PACKER, A. P.; BENTO, C. B.; CARMO, J. B. do; SILVA, F. A. de M. |
|
Afiliação: |
REGINALDO BARBOZA DA SILVA, FCA-UNESP Registro; TALINE ANTUNES, FCA-UNESP Registro; JÉSSICA SILVA ROSA, FCA-UNESP Registro; ANA PAULA CONTADOR PACKER, CNPMA; CAMILA BOLFARINI BENTO; JANAINA BRAGA DO CARMO, UFSCar; FRANCISCA ALCIVÂNIA DE MELO SILVA, FCA-UNESP Registro. |
|
Título: |
CO2, CH4 and N2O emissions after fertilizer application in banana plantations located in the Brazilian Atlantic Forest. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
Soil Use and Management, v. 38, n. 4, p. 1597-1613, 2022. |
|
ISSN: |
0266-0032 |
|
DOI: |
https://doi.org/10.1111/sum.12822 |
|
Idioma: |
Inglês |
|
Notas: |
On-line first. |
|
Conteúdo: |
Abstract: Bananas are important tropical fruits conventionally cultivated under intensive nitrogen fertilization. A current challenge is to understand the environmental impacts of this crop across the different cultivation stages considering greenhouse gas emissions. Therefore, the present study evaluated whether inorganic fertilization with ammonium sulphate and urea during different planting stages can change soil CO2, CH4 and N2O emissions. The experiment was conducted in 2018 in an Atlantic Forest region of S?o Paulo State, Brazil. We used the chamber-based methodology for gas sampling in young and established banana plantations and in a forest remnant fragment. Seasonal differences in temperature and rainfall during the sampling period resulted in a larger WFPS during the wet season. The CO2 emissions followed the rainfall variations. CH4 fluxes were mainly resulted of methanotrophy reactions. The maximum and minimum N2O fluxes were 7.38 and ?0.93?mg?m2 day?1, peaking after nitrogen fertilization. We found that the accumulated N2O fluxes from soil were greater for the two banana plantations than those observed in the forest remnant in dry and wet seasons. The highest N2O peaks were observed in the young banana plantation. CH4 uptake was 92 and 61% less in young and established banana plantations than in the forest remnant, while N2O emissions were 95 and 74% greater in young and established banana plantations than in the forest remnant. Considering the negative effect of N2O emissions, reduced rates of nitrogen application and the adoption of conservation practices should be considered in young banana plantations. MenosAbstract: Bananas are important tropical fruits conventionally cultivated under intensive nitrogen fertilization. A current challenge is to understand the environmental impacts of this crop across the different cultivation stages considering greenhouse gas emissions. Therefore, the present study evaluated whether inorganic fertilization with ammonium sulphate and urea during different planting stages can change soil CO2, CH4 and N2O emissions. The experiment was conducted in 2018 in an Atlantic Forest region of S?o Paulo State, Brazil. We used the chamber-based methodology for gas sampling in young and established banana plantations and in a forest remnant fragment. Seasonal differences in temperature and rainfall during the sampling period resulted in a larger WFPS during the wet season. The CO2 emissions followed the rainfall variations. CH4 fluxes were mainly resulted of methanotrophy reactions. The maximum and minimum N2O fluxes were 7.38 and ?0.93?mg?m2 day?1, peaking after nitrogen fertilization. We found that the accumulated N2O fluxes from soil were greater for the two banana plantations than those observed in the forest remnant in dry and wet seasons. The highest N2O peaks were observed in the young banana plantation. CH4 uptake was 92 and 61% less in young and established banana plantations than in the forest remnant, while N2O emissions were 95 and 74% greater in young and established banana plantations than in the forest remnant. Considering the negative effect o... Mostrar Tudo |
|
Palavras-Chave: |
Mata Atlântica. |
|
Thesagro: |
Banana; Clima; Cultivo Continuo; Dióxido de Carbono; Fertilizante Nitrogenado; Impacto Ambiental. |
|
Thesaurus NAL: |
Bananas; Carbon dioxide; Environmental impact; Greenhouse gas emissions; Nitrogen fertilizers; Tropical forests. |
|
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
|
Marc: |
LEADER 02789naa a2200385 a 4500
001 2143923
005 2023-07-14
008 2022 bl uuuu u00u1 u #d
022 $a0266-0032
024 7 $ahttps://doi.org/10.1111/sum.12822$2DOI
100 1 $aSILVA, R. B. da
245 $aCO2, CH4 and N2O emissions after fertilizer application in banana plantations located in the Brazilian Atlantic Forest.$h[electronic resource]
260 $c2022
500 $aOn-line first.
520 $aAbstract: Bananas are important tropical fruits conventionally cultivated under intensive nitrogen fertilization. A current challenge is to understand the environmental impacts of this crop across the different cultivation stages considering greenhouse gas emissions. Therefore, the present study evaluated whether inorganic fertilization with ammonium sulphate and urea during different planting stages can change soil CO2, CH4 and N2O emissions. The experiment was conducted in 2018 in an Atlantic Forest region of S?o Paulo State, Brazil. We used the chamber-based methodology for gas sampling in young and established banana plantations and in a forest remnant fragment. Seasonal differences in temperature and rainfall during the sampling period resulted in a larger WFPS during the wet season. The CO2 emissions followed the rainfall variations. CH4 fluxes were mainly resulted of methanotrophy reactions. The maximum and minimum N2O fluxes were 7.38 and ?0.93?mg?m2 day?1, peaking after nitrogen fertilization. We found that the accumulated N2O fluxes from soil were greater for the two banana plantations than those observed in the forest remnant in dry and wet seasons. The highest N2O peaks were observed in the young banana plantation. CH4 uptake was 92 and 61% less in young and established banana plantations than in the forest remnant, while N2O emissions were 95 and 74% greater in young and established banana plantations than in the forest remnant. Considering the negative effect of N2O emissions, reduced rates of nitrogen application and the adoption of conservation practices should be considered in young banana plantations.
650 $aBananas
650 $aCarbon dioxide
650 $aEnvironmental impact
650 $aGreenhouse gas emissions
650 $aNitrogen fertilizers
650 $aTropical forests
650 $aBanana
650 $aClima
650 $aCultivo Continuo
650 $aDióxido de Carbono
650 $aFertilizante Nitrogenado
650 $aImpacto Ambiental
653 $aMata Atlântica
700 1 $aANTUNES, T.
700 1 $aROSA, J. S.
700 1 $aPACKER, A. P.
700 1 $aBENTO, C. B.
700 1 $aCARMO, J. B. do
700 1 $aSILVA, F. A. de M.
773 $tSoil Use and Management$gv. 38, n. 4, p. 1597-1613, 2022.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio Ambiente (CNPMA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
