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Registro Completo |
Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
31/03/2016 |
Data da última atualização: |
13/04/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LOIOLA, C. M.; AZEVEDO, A. O. N.; DINIZ, L. E. C.; ARAGÃO, W. M.; AZEVEDO, C. D. de O.; SANTOS, P. H. A. D.; RAMOS, H. C. C.; PEREIRA, M. G.; RAMOS, S. R. R. |
Afiliação: |
LEANDRO EUGENIO CARDAMONE DINIZ, CPATC. |
Título: |
Genetic relationships among tall coconut palm (cocos nucifera l.) accessions of the international coconut genebank for latin america and the caribbean (ICG-LAC), evaluated using microsatellite markers (SSRs). |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
PLOS ONE, march, 2016. |
DOI: |
:10.1371 |
Idioma: |
Português |
Conteúdo: |
The diversity and genetic relationships among two accessions of tall coconut palms col-lected in Brazil and seven accessions introduced from different geographic regions of the world were analyzed using 25 microsatellite primers, 19 of which were polymorphic and detected between 4 and 10 alleles per locus, with an average of 6.57. The observed and expected heterozygosity ranged from 0.25 and 0.40 in the Rennell Islands Tall (RIT) accession to 0.54 and 0.62 in the Polynesian Tall (PYT) accession. The analysis of genetic structure resulted in the formation of five distinct groups. The first group was formed by the accessions Brazilian Tall ? Praia do Forte (BRTPF), Brazilian Tall ? Merepe (BRTMe) and West African Tall (WAT); the second group consisted of Malaysian Tall (MLT); the third group of RIT; the fourth group of Vanuatu Tall (VTT); and the fifth group of Rotuman Tall (RTMT), Tonga Tall (TONT) and PYT. The dendrogram based on the nearest-neighbor method detected the formation of two main groups and five subgroups, indicating that the genetic relationships of the accessions are based on their geographic regions of origin. The analyses revealed genetic relationships between the accessions collected in Brazil and the accession from Africa, and among palms from South East Asia and the South Pacific, con firming the common origin of these accessions. The information obtained in this study can guide decisions on germplasm conservation activities and the efficient selection of geneti cally divergent parents for use in coconut breeding programs in Brazil, which are attempting to select for disease resistance, mainly to lethal yellowing, among other characteristics. MenosThe diversity and genetic relationships among two accessions of tall coconut palms col-lected in Brazil and seven accessions introduced from different geographic regions of the world were analyzed using 25 microsatellite primers, 19 of which were polymorphic and detected between 4 and 10 alleles per locus, with an average of 6.57. The observed and expected heterozygosity ranged from 0.25 and 0.40 in the Rennell Islands Tall (RIT) accession to 0.54 and 0.62 in the Polynesian Tall (PYT) accession. The analysis of genetic structure resulted in the formation of five distinct groups. The first group was formed by the accessions Brazilian Tall ? Praia do Forte (BRTPF), Brazilian Tall ? Merepe (BRTMe) and West African Tall (WAT); the second group consisted of Malaysian Tall (MLT); the third group of RIT; the fourth group of Vanuatu Tall (VTT); and the fifth group of Rotuman Tall (RTMT), Tonga Tall (TONT) and PYT. The dendrogram based on the nearest-neighbor method detected the formation of two main groups and five subgroups, indicating that the genetic relationships of the accessions are based on their geographic regions of origin. The analyses revealed genetic relationships between the accessions collected in Brazil and the accession from Africa, and among palms from South East Asia and the South Pacific, con firming the common origin of these accessions. The information obtained in this study can guide decisions on germplasm conservation activities and the efficient selection of ... Mostrar Tudo |
Palavras-Chave: |
Cocos nucifera L. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02494naa a2200241 a 4500 001 2042280 005 2017-04-13 008 2016 bl uuuu u00u1 u #d 024 7 $a:10.1371$2DOI 100 1 $aLOIOLA, C. M. 245 $aGenetic relationships among tall coconut palm (cocos nucifera l.) accessions of the international coconut genebank for latin america and the caribbean (ICG-LAC), evaluated using microsatellite markers (SSRs).$h[electronic resource] 260 $c2016 520 $aThe diversity and genetic relationships among two accessions of tall coconut palms col-lected in Brazil and seven accessions introduced from different geographic regions of the world were analyzed using 25 microsatellite primers, 19 of which were polymorphic and detected between 4 and 10 alleles per locus, with an average of 6.57. The observed and expected heterozygosity ranged from 0.25 and 0.40 in the Rennell Islands Tall (RIT) accession to 0.54 and 0.62 in the Polynesian Tall (PYT) accession. The analysis of genetic structure resulted in the formation of five distinct groups. The first group was formed by the accessions Brazilian Tall ? Praia do Forte (BRTPF), Brazilian Tall ? Merepe (BRTMe) and West African Tall (WAT); the second group consisted of Malaysian Tall (MLT); the third group of RIT; the fourth group of Vanuatu Tall (VTT); and the fifth group of Rotuman Tall (RTMT), Tonga Tall (TONT) and PYT. The dendrogram based on the nearest-neighbor method detected the formation of two main groups and five subgroups, indicating that the genetic relationships of the accessions are based on their geographic regions of origin. The analyses revealed genetic relationships between the accessions collected in Brazil and the accession from Africa, and among palms from South East Asia and the South Pacific, con firming the common origin of these accessions. The information obtained in this study can guide decisions on germplasm conservation activities and the efficient selection of geneti cally divergent parents for use in coconut breeding programs in Brazil, which are attempting to select for disease resistance, mainly to lethal yellowing, among other characteristics. 653 $aCocos nucifera L 700 1 $aAZEVEDO, A. O. N. 700 1 $aDINIZ, L. E. C. 700 1 $aARAGÃO, W. M. 700 1 $aAZEVEDO, C. D. de O. 700 1 $aSANTOS, P. H. A. D. 700 1 $aRAMOS, H. C. C. 700 1 $aPEREIRA, M. G. 700 1 $aRAMOS, S. R. R. 773 $tPLOS ONE, march, 2016.
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Embrapa Tabuleiros Costeiros (CPATC) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
Data corrente: |
17/09/2013 |
Data da última atualização: |
22/02/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
GOMES, S. G.; TORRES, A. G.; GODOY, R.; PACHECO, S.; CARVALHO, J.; NUTTI, M. |
Afiliação: |
SUELLEN GOMES, UFRJ; ALEXANDRE GUEDES TORRES, UFRJ; RONOEL LUIZ DE OLIVEIRA GODOY, CTAA; SIDNEY PACHECO, CTAA; JOSE LUIZ VIANA DE CARVALHO, CTAA; MARILIA REGINI NUTTI, CTAA. |
Título: |
Effects of boiling and frying on the bioaccessibility of B-carotene in yellow-fleshed cassava roots (Manihot esculenta Crantz cv. BRS Jari). |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Food and Nutrition Bulletin, v. 34, n. 1, p. 65-74, 2013. |
Idioma: |
Inglês |
Conteúdo: |
Background. The effects of boiling and frying on the bioaccessibility of all-trans-?-carotene in biofortified BRS Jari cassava roots have not been investigated, although these are conventional methods of cassava preparation. Objective. The aims of the present study were to investigate B-carotene micellarization efficiency of yellowfleshed BRS Jari cassava roots after boiling and frying, as an indicator of the bioaccessibility of this carotenoid, and to apply fluorescence microscopy to investigate B-carotene in the emulsified fraction. Methods. Uncooked, boiled, and fried cassava roots were digested in vitro for the evaluation, by reversedphase high-performance liquid chromatography (HPLC), of the efficiency of micellarization of all-trans-B-carotene in BRS Jari cassava roots. Fluorescence microscopy of the micellar fraction was used to confirm the presence of B-carotene in the emulsified fraction and to observe the structure of the microemulsion from the boiled and fried cassava samples. Results. Fried cassava roots showed the highest (p < .05) micellarization efficiency for total carotenoids and alltrans-B-carotene (14.1 ± 2.25% and 14.37 ± 2.44%, respectively), compared with boiled and raw samples. Fluorescence microscopy showed that after in vitro digestion there were no carotenoid crystals in the micellar fraction, but rather that this fraction presented a biphasic system compatible with emulsified carotenoids, which was consistent with the expected high bioavailability of B-carotene in this fraction. MenosBackground. The effects of boiling and frying on the bioaccessibility of all-trans-?-carotene in biofortified BRS Jari cassava roots have not been investigated, although these are conventional methods of cassava preparation. Objective. The aims of the present study were to investigate B-carotene micellarization efficiency of yellowfleshed BRS Jari cassava roots after boiling and frying, as an indicator of the bioaccessibility of this carotenoid, and to apply fluorescence microscopy to investigate B-carotene in the emulsified fraction. Methods. Uncooked, boiled, and fried cassava roots were digested in vitro for the evaluation, by reversedphase high-performance liquid chromatography (HPLC), of the efficiency of micellarization of all-trans-B-carotene in BRS Jari cassava roots. Fluorescence microscopy of the micellar fraction was used to confirm the presence of B-carotene in the emulsified fraction and to observe the structure of the microemulsion from the boiled and fried cassava samples. Results. Fried cassava roots showed the highest (p < .05) micellarization efficiency for total carotenoids and alltrans-B-carotene (14.1 ± 2.25% and 14.37 ± 2.44%, respectively), compared with boiled and raw samples. Fluorescence microscopy showed that after in vitro digestion there were no carotenoid crystals in the micellar fraction, but rather that this fraction presented a biphasic system compatible with emulsified carotenoids, which was consistent with the expected high bioavailability ... Mostrar Tudo |
Palavras-Chave: |
Betacaroteno; Biodisponibilidade; Biofortificação; Carotenóides; Deficiência de vitamina A; Digestão in vitro. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02299naa a2200253 a 4500 001 1966374 005 2016-02-22 008 2013 bl uuuu u00u1 u #d 100 1 $aGOMES, S. G. 245 $aEffects of boiling and frying on the bioaccessibility of B-carotene in yellow-fleshed cassava roots (Manihot esculenta Crantz cv. BRS Jari). 260 $c2013 520 $aBackground. The effects of boiling and frying on the bioaccessibility of all-trans-?-carotene in biofortified BRS Jari cassava roots have not been investigated, although these are conventional methods of cassava preparation. Objective. The aims of the present study were to investigate B-carotene micellarization efficiency of yellowfleshed BRS Jari cassava roots after boiling and frying, as an indicator of the bioaccessibility of this carotenoid, and to apply fluorescence microscopy to investigate B-carotene in the emulsified fraction. Methods. Uncooked, boiled, and fried cassava roots were digested in vitro for the evaluation, by reversedphase high-performance liquid chromatography (HPLC), of the efficiency of micellarization of all-trans-B-carotene in BRS Jari cassava roots. Fluorescence microscopy of the micellar fraction was used to confirm the presence of B-carotene in the emulsified fraction and to observe the structure of the microemulsion from the boiled and fried cassava samples. Results. Fried cassava roots showed the highest (p < .05) micellarization efficiency for total carotenoids and alltrans-B-carotene (14.1 ± 2.25% and 14.37 ± 2.44%, respectively), compared with boiled and raw samples. Fluorescence microscopy showed that after in vitro digestion there were no carotenoid crystals in the micellar fraction, but rather that this fraction presented a biphasic system compatible with emulsified carotenoids, which was consistent with the expected high bioavailability of B-carotene in this fraction. 653 $aBetacaroteno 653 $aBiodisponibilidade 653 $aBiofortificação 653 $aCarotenóides 653 $aDeficiência de vitamina A 653 $aDigestão in vitro 700 1 $aTORRES, A. G. 700 1 $aGODOY, R. 700 1 $aPACHECO, S. 700 1 $aCARVALHO, J. 700 1 $aNUTTI, M. 773 $tFood and Nutrition Bulletin$gv. 34, n. 1, p. 65-74, 2013.
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