Data/hora: 25/06/2024 10:59:52

Biblioteca(s): 	Embrapa Meio Ambiente.	
Data corrente: 	05/04/2021	
Data da última atualização: 	05/04/2021	
Autoria: 	MEDEIROS, A. M. Z.; KHAN, L. U.; SILVA, G. H. da; OSPINA, C. A.; ALVES, O. L.; CASTRO, V. L. S. S. de; MARTINEZ, D. S. T.	
Afiliação: 	ALINE MARIA ZIGIOTTO DE MEDEIROS, CNPEM; LATIF ULLAH KHAN, CNPEM; GABRIELA HELENA DA SILVA, CNPEM; CARLOS ALBERTO OSPINA, CNPEM; OSWALDO LUIZ ALVES, IQ-UNICAMP; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; DIEGO STÉFANI TEODORO MARTINEZ, CNPEM.	
Título: 	Graphene oxide-silver nanoparticle hybrid material: an integrated nanosafety study in zebrafish embryos.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Ecotoxicology and Environmental Safety, v. 209, article 111776, 2021.	
ISSN: 	0147-6513	
DOI: 	https://doi.org/10.1016/j.ecoenv.2020.111776	
Idioma: 	Inglês	
Conteúdo: 	Abstract: This work reports an integrated nanosafety study including the synthesis and characterization of the graphene oxide-silver nanoparticle hybrid material (GO-AgNPs) and its nano-ecotoxicity evaluation in the zebrafish embryo model. The influences of natural organic matter (NOM) and a chorion embryo membrane were considered in this study, looking towards more environmentally realistic scenarios and standardized nanotoxicity testing. The nanohybrid was successfully synthesized using the NaBH4 aqueous method, and AgNPs (~ 5.8 nm) were evenly distributed over the GO surface. GO-AgNPs showed a dose-response acute toxicity: the LC50 was 1.5 mg L-1 for chorionated embryos. The removal of chorion, however, increased this toxic effect by 50%. Furthermore, the presence of NOM mitigated mortality, and LC50 for GO-AgNPs changed respectively from 2.3 to 1.2 mg L-1 for chorionated and de-chorionated embryos. Raman spectroscopy confirmed the ingestion of GO by embryos; but without displaying acute toxicity up to 100 mg L-1, indicating that the silver drove toxicity down. Additionally, it was observed that silver nanoparticle dissolution has a minimal effect on these observed toxicity results. Finally, understanding the influence of chorion membranes and NOM is a critical step towards the standardization of testing for zebrafish embryo toxicity in safety assessments and regulatory issues.	
Palavras-Chave: 	Alternative methods; Grafeno; Nanoecotoxicology; Nanohybrids; Nanomateriais; Nanotoxicity.	
Thesagro: 	Composto de Carbono; Peixe de Água Doce; Toxidez.	
Thesaurus Nal: 	chorion; Danio rerio; Ecotoxicology; Graphene oxide; Nanomaterials.	
Categoria do assunto: 	P Recursos Naturais, Ciências Ambientais e da Terra	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/222338/1/Castro-Graphene-oxide-2021.pdf	
Registro original: 	Embrapa Meio Ambiente (CNPMA)	
Exemplares: 	CNPMA - (16956-1) - UPC - AP - DD -  -  -  - DIS