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Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
19/05/1995 |
Data da última atualização: |
07/07/2006 |
Autoria: |
MOUSASTICOSHVILY JUNIOR, I.; GRACA, L. R. |
Título: |
A comercializacao da virola do estuario amazonico. |
Ano de publicação: |
1992 |
Fonte/Imprenta: |
In: ENCONTRO BRASILEIRO DE ECONOMIA E PLANEJAMENTO FLORESTAL, 2., 1991, Curitiba. Anais. Colombo: EMBRAPA-CNPF, 1992. v.2. p.363-374. |
Idioma: |
Português |
Categoria do assunto: |
-- |
Marc: |
LEADER 00434naa a2200121 a 4500 001 1300159 005 2006-07-07 008 1992 bl uuuu u00u1 u #d 100 1 $aMOUSASTICOSHVILY JUNIOR, I. 245 $aA comercializacao da virola do estuario amazonico. 260 $c1992 700 1 $aGRACA, L. R. 773 $tIn: ENCONTRO BRASILEIRO DE ECONOMIA E PLANEJAMENTO FLORESTAL, 2., 1991, Curitiba. Anais. Colombo: EMBRAPA-CNPF, 1992.$gv.2. p.363-374.
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Embrapa Florestas (CNPF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Instrumentação; Embrapa Milho e Sorgo. |
Data corrente: |
21/07/2023 |
Data da última atualização: |
30/11/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
VELLOSO, C. C. V.; CAMARGO, B. C. P.; SOUSA, M. D. B.; BUFFO, M. M.; OLIVEIRA-PAIVA, C. A.; FARINAS, C. S.; BALDINO, A. C. |
Afiliação: |
CAMILA CRISTINA VIEIRA VELLOSO, Universidade Federal de São Carlos; BRUNA CERRI PEREIRA CAMARGO, Universidade Federal de São Carlos; MARINA DEL BIANCO SOUSA, Universidade Federal de São Carlos; MARIANE MOLINA BUFFO, Universidade Federal de São Carlos; CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS; CRISTIANE SANCHEZ FARINAS, CNPDIA; ALBERTO COLLI BADINO, Universidade Federal de São Carlos. |
Título: |
High yield of heat-resistant spores of Bacillus megaterium in bioreactors. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Biochemical Engineering Journal, v. 198, 109030, 2023. |
DOI: |
https://doi.org/10.1016/j.bej.2023.109030 |
Idioma: |
Inglês |
Conteúdo: |
Microbial inoculants have been proposed as a sustainable and cost-effective alternative to conventional agricultural practices. However, the effectiveness of these bio-based products can be negatively impacted by reduced cell viability during product development, storage, and commercialization. To address these limitations, it is critical to identify and optimize the cultivation conditions that can lead to the production of highly resistant spores for use as inoculants. In this study, optimal cultivation conditions for obtaining Bacillus megaterium B119 spores in bioreactors were reached, with increases in the sporulation efficiency and heat resistance. The best nutrient medium and optimal temperature and initial pH conditions were obtained on shake flask experiments and subsequently validated in two bench-scale bioreactor models (stirred and aerated tank and the bubble column). The optimized temperature and initial pH were found to be 37 °C and 5.6. The highest concentration of heat-resistant Bacillus spores of 1.31·1012 CFU/mL was obtained in the bubble column bioreactor with a sporulation efficiency of 100.0%, a heat resistance of 78.4%, and an energetic efficiency on spores production of 4.83·1010 CFU/J. The production of highly resistant spores can significantly improve the survival rate of microorganisms during inoculant production and application, thereby enhancing their effectiveness in the field. |
Palavras-Chave: |
Engenharia de bioprocessos; Microbial inoculant; Sporulation efficiency. |
Thesagro: |
Bactéria; Esporo; Esporulação; Inoculação. |
Thesaurus NAL: |
Bacillus megaterium; Bioprocess engineering. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02369naa a2200313 a 4500 001 2155185 005 2023-11-30 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.bej.2023.109030$2DOI 100 1 $aVELLOSO, C. C. V. 245 $aHigh yield of heat-resistant spores of Bacillus megaterium in bioreactors.$h[electronic resource] 260 $c2023 520 $aMicrobial inoculants have been proposed as a sustainable and cost-effective alternative to conventional agricultural practices. However, the effectiveness of these bio-based products can be negatively impacted by reduced cell viability during product development, storage, and commercialization. To address these limitations, it is critical to identify and optimize the cultivation conditions that can lead to the production of highly resistant spores for use as inoculants. In this study, optimal cultivation conditions for obtaining Bacillus megaterium B119 spores in bioreactors were reached, with increases in the sporulation efficiency and heat resistance. The best nutrient medium and optimal temperature and initial pH conditions were obtained on shake flask experiments and subsequently validated in two bench-scale bioreactor models (stirred and aerated tank and the bubble column). The optimized temperature and initial pH were found to be 37 °C and 5.6. The highest concentration of heat-resistant Bacillus spores of 1.31·1012 CFU/mL was obtained in the bubble column bioreactor with a sporulation efficiency of 100.0%, a heat resistance of 78.4%, and an energetic efficiency on spores production of 4.83·1010 CFU/J. The production of highly resistant spores can significantly improve the survival rate of microorganisms during inoculant production and application, thereby enhancing their effectiveness in the field. 650 $aBacillus megaterium 650 $aBioprocess engineering 650 $aBactéria 650 $aEsporo 650 $aEsporulação 650 $aInoculação 653 $aEngenharia de bioprocessos 653 $aMicrobial inoculant 653 $aSporulation efficiency 700 1 $aCAMARGO, B. C. P. 700 1 $aSOUSA, M. D. B. 700 1 $aBUFFO, M. M. 700 1 $aOLIVEIRA-PAIVA, C. A. 700 1 $aFARINAS, C. S. 700 1 $aBALDINO, A. C. 773 $tBiochemical Engineering Journal$gv. 198, 109030, 2023.
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