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Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
02/07/1999 |
Data da última atualização: |
02/07/1999 |
Autoria: |
BRODEUR, J.; McNEIL, J. N. |
Título: |
Biotic and abiotic factors involved in diapause induction of the parasitoid, Aphidius nigripes (Hymenoptera: Aphidiidae). |
Ano de publicação: |
1989 |
Fonte/Imprenta: |
Journal of Insect Phisiologist, Devon, v.35, n.12, p.969-974, 1989. |
Idioma: |
Inglês |
Palavras-Chave: |
Diapause induction; Fatores abioticos; Fatores bioticos; Inducao a diapausa; Insect seasonality; Parasitoid; Parasitoide; Sazonalidade. |
Thesagro: |
Inseto. |
Thesaurus Nal: |
Aphidiidae; Aphidius nigripes; diapause. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00788naa a2200265 a 4500 001 1303327 005 1999-07-02 008 1989 bl --- 0-- u #d 100 1 $aBRODEUR, J. 245 $aBiotic and abiotic factors involved in diapause induction of the parasitoid, Aphidius nigripes (Hymenoptera$bAphidiidae). 260 $c1989 650 $aAphidiidae 650 $aAphidius nigripes 650 $adiapause 650 $aInseto 653 $aDiapause induction 653 $aFatores abioticos 653 $aFatores bioticos 653 $aInducao a diapausa 653 $aInsect seasonality 653 $aParasitoid 653 $aParasitoide 653 $aSazonalidade 700 1 $aMcNEIL, J. N. 773 $tJournal of Insect Phisiologist, Devon$gv.35, n.12, p.969-974, 1989.
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Registro original: |
Embrapa Florestas (CNPF) |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
14/01/2011 |
Data da última atualização: |
17/04/2018 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
VASCONCELOS, E. A. R.; SANTANA, C. G.; GODOY, C. V.; SILVA, M. S.; MOREIRA, L. R. S.; OLIVEIRA-NETO, OSMUNDO B.; PRICE, D.; FITCHES, E.; FILHO, E. X. F.; MEHTA, A.; GATEHOUSE, J. A.; GROSSI-DE-SA, M. F. |
Afiliação: |
ÉRICO A. R. VASCONCELOS, UNB; CELSO G. SANTANA; CLAUDIA VIEIRA GODOY, CNPSO; MARILIA SANTOS SILVA, CPAC; LEONORA R. S. MOREIRA, UNB; OSMUNDO B. OLIVEIRA-NETO; DANIEL PRICE, Durham University; ELAINE FITCHES, Durham University; EDIVALDO X. F. FILHO, UNB; ANGELA MEHTA DOS REIS, CENARGEN; JOHN A. GATEHOUSE, Durham University; MARIA FATIMA GROSSI DE SA, CENARGEN. |
Título: |
A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. [Programa e resumos]: errata. Brasília, DF: SBBiotec, 2010. |
Idioma: |
Inglês |
Conteúdo: |
Asian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. In order to find a biochemical candidate to such enterprise, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the pGAPZa-B vector for expression in Pichia pastoris. A cDNA encoding a chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP), was isolated from leaves. The amino acid sequence predicts a (ß/a)8 topology common to Class III Chitinases (glycoside hydrolase family 18 proteins; GH18), and shares similarity with other GH18 members, although it lacks the glutamic acid residue essential for catalysis, which is replaced by glutamine. CaclXIP was expressed as a recombinant protein in Pichia pastoris. Enzymatic assay using purified protein showed that CaclXIP had only residual chitinolytic activity. However, it inhibited xylanases from Acrophialophora nainiana by approx. 60% when present at sub-molar equivalence (12:1 enzyme:inhibitor ratio). Additionally, CaclXIP at 1.5 ?g/?L inhibited by 45% the germination of spores of Phakopsora pachyrhizi. These results show that CaclXIP belongs to a class of naturally inactive chitinases that have evolved to act in plant cell defence as xylanase inhibitors. Its role on inhibiting germination of fungal spores makes of it an eligible candidate gene for Asian rust control. |
Palavras-Chave: |
Asian rust. |
Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/31109/1/cbb.errata.cvg.pdf
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Marc: |
LEADER 02303nam a2200253 a 4500 001 1873147 005 2018-04-17 008 2010 bl uuuu u00u1 u #d 100 1 $aVASCONCELOS, E. A. R. 245 $aA new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. 260 $aIn: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. [Programa e resumos]: errata. Brasília, DF: SBBiotec$c2010 520 $aAsian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. In order to find a biochemical candidate to such enterprise, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the pGAPZa-B vector for expression in Pichia pastoris. A cDNA encoding a chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP), was isolated from leaves. The amino acid sequence predicts a (ß/a)8 topology common to Class III Chitinases (glycoside hydrolase family 18 proteins; GH18), and shares similarity with other GH18 members, although it lacks the glutamic acid residue essential for catalysis, which is replaced by glutamine. CaclXIP was expressed as a recombinant protein in Pichia pastoris. Enzymatic assay using purified protein showed that CaclXIP had only residual chitinolytic activity. However, it inhibited xylanases from Acrophialophora nainiana by approx. 60% when present at sub-molar equivalence (12:1 enzyme:inhibitor ratio). Additionally, CaclXIP at 1.5 ?g/?L inhibited by 45% the germination of spores of Phakopsora pachyrhizi. These results show that CaclXIP belongs to a class of naturally inactive chitinases that have evolved to act in plant cell defence as xylanase inhibitors. Its role on inhibiting germination of fungal spores makes of it an eligible candidate gene for Asian rust control. 653 $aAsian rust 700 1 $aSANTANA, C. G. 700 1 $aGODOY, C. V. 700 1 $aSILVA, M. S. 700 1 $aMOREIRA, L. R. S. 700 1 $aOLIVEIRA-NETO, OSMUNDO B. 700 1 $aPRICE, D. 700 1 $aFITCHES, E. 700 1 $aFILHO, E. X. F. 700 1 $aMEHTA, A. 700 1 $aGATEHOUSE, J. A. 700 1 $aGROSSI-DE-SA, M. F.
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Embrapa Soja (CNPSO) |
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