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11. | | RIBEIRO, D. G.; CUNHA, G. C. R. da; SANTOS, C. dos; SILVA, L. P. da; OLIVEIRA NETO, O. B. de; LABUTO, L. B. D.; REIS, A. M. dos. Brassica oleracea resistance-related proteins identified at an early stage of black rot disease. Physiological and Molecular Plant Pathology, v. 104, p. 9-14, 2018. Na publicação: Luciano Paulino Silva e Angela Mehta. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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12. | | MIRANDA, V. J.; VIANA, A. A. B.; OLIVEIRA NETO, O. B de; CARNEIRO, R. M. D. G.; SA, M. F. G. de; FRAGOSO, R. da R. Codificador transcrito da enzima de conjugação a ubiquitina (E2) ativado em galhas de soja inoculada com Meloidogyne incognita. In: CONGRESSO BRASILEIRO DE NEMATOLOGIA, 29., 2011, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Nematologia, 2011. p. 152-153. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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16. | | OLIVEIRA NETO, O. B. de; BATISTA, J. A. N.; RIGDEN, D. J.; FRAGOSO, R. da R.; SILVA, R. O. da; MONNERAT, R. G.; SA, M. F. G. de. A large, diverse family of serine protease genes expressed in cotton boll weevil (Anthonomus grandis): implications for design of pest-resistant transgenic cotton plants. In: ENCONTRO LATINO-AMERICANO DE BIOTECNOLOGIA VEGETAL: REDBIO, 4., 2001, Goiania. Programa e resumos. [S.l.:s.n.], 2001. p. 178. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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18. | | ALVAREZ-PIZARRO, J. C.; GOMES FILHO, E.; PRISCO, J. T.; GROSSI DE SA, M. F.; OLIVEIRA NETO, O. B. de; ROCHA FRAGOSO, R. da. Plasma membrane H+-ATPase in sorghum roots as affected by potassium deficiency and nitrogen sources. Biologia Plantarum, v. 58, n. 3, p. 507-514, 2014. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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19. | | OLIVEIRA, U. A. de; DEL SARTO, R. P.; OLIVEIRA NETO, O. B. de; COUTINHO, M. V.; SA, M. F. G. de; SILVA, M. C. M. da. Novos inibidores de ?-amilases gerados por evolução molecular dirigida: potencial para o controle de bruquídeos. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 14., 2009, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2009. Resumo 026. p. 62 Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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20. | | PAULA, A. W. M.; VIANA, A. A.; MULINARI, F.; LIMA, J. N.; OLIVEIRA NETO, O. B.; DE FREITAS, S. M.; GROSSI DE SÁ, M. F. Expression of the btci gene (Bowman-birk type seed trypsin and Chymotrypsin inhibitor) aiming cotton bool weevil control. In: ANNUAL MEETING OF SBBQ, 37.; CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia, SP. Abstracts... Águas de Lindóia: SBBq, 2008. 1 CD-ROM. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
17/12/2014 |
Data da última atualização: |
17/12/2014 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
ALVAREZ-PIZARRO, J. C.; GOMES FILHO, E.; PRISCO, J. T.; GROSSI DE SA, M. F.; OLIVEIRA NETO, O. B. de; FRAGOSO, R. da R. |
Afiliação: |
MARIA FATIMA GROSSI DE SA, CENARGEN; RODRIGO DA ROCHA FRAGOSO, CPAC. |
Título: |
Plasma membrane H+-ATPase in sorghum roots as affected by potassium deficiency and nitrogen sources. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Biologia Plantarum, v. 58, n. 3, p. 507-514, 2014. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: We studied the influence of inorganic nitrogen sources (NO3- or NH4+) and potassium deficiency on expression and activity of plasma membrane (PM) H+-ATPase in sorghum roots. After 15 d of cultivation at 0.2 mM K+, the plants were transferred to solutions lacking K+ for 2 d. Then, K+ depletion assays were performed in the presence or absence of vanadate. Further, PMs from K+-starved roots were extracted and used for the kinetic characterization of ATP hydrolytic activity and the immunodetection of PM H+-ATPase. Two major genes coding PM H+-ATPase (SBA1 and SBA2) were analyzed by real-time PCR. PM H+-ATPase exhibited a higher Vmax and Km in NH4+-fed roots compared with NO3--fed roots. The optimum pH of the enzyme was slightly lower in NO3- -fed roots than in NH4+ -fed roots. The vanadate sensitivity was similar. The expressions of SBA1 and SBA2 were increased in roots grown under NH4+. Concomitantly, an increased content of the enzyme in PM was observed. The initial rate of K+ uptake did not differ between plants grown with NO3- or NH4+, but it was significantly reduced by vanadate in NH4+-grown plants. |
Palavras-Chave: |
Nitrato de Amonia. |
Thesagro: |
Fertilizante nitrogenado; Potássio; Sorghum Bicolor; Sorgo. |
Thesaurus NAL: |
Ammonium nitrate; Nitrate fertilizers; Potassium. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
Marc: |
LEADER 01933naa a2200277 a 4500 001 2003022 005 2014-12-17 008 2014 bl uuuu u00u1 u #d 100 1 $aALVAREZ-PIZARRO, J. C. 245 $aPlasma membrane H+-ATPase in sorghum roots as affected by potassium deficiency and nitrogen sources. 260 $c2014 520 $aAbstract: We studied the influence of inorganic nitrogen sources (NO3- or NH4+) and potassium deficiency on expression and activity of plasma membrane (PM) H+-ATPase in sorghum roots. After 15 d of cultivation at 0.2 mM K+, the plants were transferred to solutions lacking K+ for 2 d. Then, K+ depletion assays were performed in the presence or absence of vanadate. Further, PMs from K+-starved roots were extracted and used for the kinetic characterization of ATP hydrolytic activity and the immunodetection of PM H+-ATPase. Two major genes coding PM H+-ATPase (SBA1 and SBA2) were analyzed by real-time PCR. PM H+-ATPase exhibited a higher Vmax and Km in NH4+-fed roots compared with NO3--fed roots. The optimum pH of the enzyme was slightly lower in NO3- -fed roots than in NH4+ -fed roots. The vanadate sensitivity was similar. The expressions of SBA1 and SBA2 were increased in roots grown under NH4+. Concomitantly, an increased content of the enzyme in PM was observed. The initial rate of K+ uptake did not differ between plants grown with NO3- or NH4+, but it was significantly reduced by vanadate in NH4+-grown plants. 650 $aAmmonium nitrate 650 $aNitrate fertilizers 650 $aPotassium 650 $aFertilizante nitrogenado 650 $aPotássio 650 $aSorghum Bicolor 650 $aSorgo 653 $aNitrato de Amonia 700 1 $aGOMES FILHO, E. 700 1 $aPRISCO, J. T. 700 1 $aGROSSI DE SA, M. F. 700 1 $aOLIVEIRA NETO, O. B. de 700 1 $aFRAGOSO, R. da R. 773 $tBiologia Plantarum$gv. 58, n. 3, p. 507-514, 2014.
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