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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
11/04/2005 |
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Data da última atualização: |
18/12/2019 |
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Autoria: |
SILVA, E. R. da. |
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Afiliação: |
Elizabete Rodrigues da Silva, CNPC. |
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Título: |
Genotipagem e avaliação do potencial enterotoxigênico de amostras de Staphylococcus aureus isolados de mastite caprina e bovina. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
2004. |
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Páginas: |
57 f. |
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Idioma: |
Português |
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Notas: |
Tese (Doutorado em Ciência Animal) - Universidade Federal de Minas Gerais, Escola de Veterinária, Belo Horizonte. Orientador: Nivaldo da Silva. |
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Conteúdo: |
Resumo: O objetivo deste trabalho foi o de identificar subtipos de Staphylococcus aureus isolados de mastite caprina e bovina, por meio da análise do polimorfismo do gene da proteína coagulase. Adicionalmente, pesquisou-se a presença de genes que codificam para as enterotoxinas estafllocócicas dos tipos A, B e C. Utilizaram-se 36 amostras de S. aureus isoladas do leite de cabras pertencentes a rebanhos localizados nas regiões Norte do Ceará e Serrana do Rio de Janeiro e 64 amostras isoladas do leite de vacas pertencentes a rebanhos localizados em municípios de Minas Gerais. Dentre os isolados de origem caprina identificaram-se 11 diferentes genótipos, sendo que nos rebanhos da região Norte do Ceará o tipo predominante foi encontrado em 60% das amostras, enquanto, nos rebanhos da região Serrana, os dois tipos mais freqüentes foram encontrados em 62,5% das amostras. A análise filogenética dos tipos indicou urna relação entre os mesmos. Dentre as amostras de origem bovina foram identificados 49 genótipos com os 10 mais freqüentes agrupando 39% das amostras. A presença de algum dos genes que codificam para as enterotoxinas pesquisadas foi detectada em 37% das amostras. Dos isolados de origem bovina, 6,3% co-amplificaram os genes sea e seb, e 3,1% foram positivas para o gene seco das amostras de origem caprina, 86% foram positivas para a presença do gene seco. A produção in vitro da enterotoxina foi detectada em todas as amostras nas quais o gene correspondente estava presente. Abstracts: The aim of this study was to identify subtypes of Staphylococcus aureus isolated from goat and bovine mastitis, by analyzing the polymorphism of coagulase gene protein. Additionally, surveyed for the presence of genes coding for staphylococcal enterotoxin types A, B and C. We used 36 strains of Staphylococcus aureus isolated from goats belonging to herds in the North of Ceará and Serrana do Rio de Janeiro and 64 strains isolated from milk of cows belonging to herds in the municipalities of Minas Gerais. Among the isolates of caprine origin are indentified 11 different genotypes, and the herds from northern Ceara predominate type was found in 60% of amostrasm while in flocks of mountain region, the two types indicated UNMA relationship between them. Among the samples of bovine origin were identified 49 genotypes with 10 more friquentes grouping 39% of samples. The presence of some genes encoding enterotoxins was investigated detactada in 37% of positive samples for gene sec. Samples of goat origin, 86% were positive for the gene sec. Production en vitro enterotoxin was detected in all samples in which the corresponding gene was present MenosResumo: O objetivo deste trabalho foi o de identificar subtipos de Staphylococcus aureus isolados de mastite caprina e bovina, por meio da análise do polimorfismo do gene da proteína coagulase. Adicionalmente, pesquisou-se a presença de genes que codificam para as enterotoxinas estafllocócicas dos tipos A, B e C. Utilizaram-se 36 amostras de S. aureus isoladas do leite de cabras pertencentes a rebanhos localizados nas regiões Norte do Ceará e Serrana do Rio de Janeiro e 64 amostras isoladas do leite de vacas pertencentes a rebanhos localizados em municípios de Minas Gerais. Dentre os isolados de origem caprina identificaram-se 11 diferentes genótipos, sendo que nos rebanhos da região Norte do Ceará o tipo predominante foi encontrado em 60% das amostras, enquanto, nos rebanhos da região Serrana, os dois tipos mais freqüentes foram encontrados em 62,5% das amostras. A análise filogenética dos tipos indicou urna relação entre os mesmos. Dentre as amostras de origem bovina foram identificados 49 genótipos com os 10 mais freqüentes agrupando 39% das amostras. A presença de algum dos genes que codificam para as enterotoxinas pesquisadas foi detectada em 37% das amostras. Dos isolados de origem bovina, 6,3% co-amplificaram os genes sea e seb, e 3,1% foram positivas para o gene seco das amostras de origem caprina, 86% foram positivas para a presença do gene seco. A produção in vitro da enterotoxina foi detectada em todas as amostras nas quais o gene correspondente estava presente. A... Mostrar Tudo |
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Palavras-Chave: |
Enterodoxina estafilocócica; Genotipagem; Genotypes. |
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Thesagro: |
Bovino; Caprino; Doença animal; Mamite; Staphylococcus aureus. |
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Thesaurus Nal: |
Animal diseases; Bovine mastitis; Goats; Mammary gland diseases; Mastitis. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162613/1/CNPC-2014-Genotipagem.pdf
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Marc: |
LEADER 03603nam a2200289 a 4500
001 1531104
005 2019-12-18
008 2004 bl uuuu m 00u1 u #d
100 1 $aSILVA, E. R. da
245 $aGenotipagem e avaliação do potencial enterotoxigênico de amostras de Staphylococcus aureus isolados de mastite caprina e bovina.
260 $a2004.$c2004
300 $a57 f.
500 $aTese (Doutorado em Ciência Animal) - Universidade Federal de Minas Gerais, Escola de Veterinária, Belo Horizonte. Orientador: Nivaldo da Silva.
520 $aResumo: O objetivo deste trabalho foi o de identificar subtipos de Staphylococcus aureus isolados de mastite caprina e bovina, por meio da análise do polimorfismo do gene da proteína coagulase. Adicionalmente, pesquisou-se a presença de genes que codificam para as enterotoxinas estafllocócicas dos tipos A, B e C. Utilizaram-se 36 amostras de S. aureus isoladas do leite de cabras pertencentes a rebanhos localizados nas regiões Norte do Ceará e Serrana do Rio de Janeiro e 64 amostras isoladas do leite de vacas pertencentes a rebanhos localizados em municípios de Minas Gerais. Dentre os isolados de origem caprina identificaram-se 11 diferentes genótipos, sendo que nos rebanhos da região Norte do Ceará o tipo predominante foi encontrado em 60% das amostras, enquanto, nos rebanhos da região Serrana, os dois tipos mais freqüentes foram encontrados em 62,5% das amostras. A análise filogenética dos tipos indicou urna relação entre os mesmos. Dentre as amostras de origem bovina foram identificados 49 genótipos com os 10 mais freqüentes agrupando 39% das amostras. A presença de algum dos genes que codificam para as enterotoxinas pesquisadas foi detectada em 37% das amostras. Dos isolados de origem bovina, 6,3% co-amplificaram os genes sea e seb, e 3,1% foram positivas para o gene seco das amostras de origem caprina, 86% foram positivas para a presença do gene seco. A produção in vitro da enterotoxina foi detectada em todas as amostras nas quais o gene correspondente estava presente. Abstracts: The aim of this study was to identify subtypes of Staphylococcus aureus isolated from goat and bovine mastitis, by analyzing the polymorphism of coagulase gene protein. Additionally, surveyed for the presence of genes coding for staphylococcal enterotoxin types A, B and C. We used 36 strains of Staphylococcus aureus isolated from goats belonging to herds in the North of Ceará and Serrana do Rio de Janeiro and 64 strains isolated from milk of cows belonging to herds in the municipalities of Minas Gerais. Among the isolates of caprine origin are indentified 11 different genotypes, and the herds from northern Ceara predominate type was found in 60% of amostrasm while in flocks of mountain region, the two types indicated UNMA relationship between them. Among the samples of bovine origin were identified 49 genotypes with 10 more friquentes grouping 39% of samples. The presence of some genes encoding enterotoxins was investigated detactada in 37% of positive samples for gene sec. Samples of goat origin, 86% were positive for the gene sec. Production en vitro enterotoxin was detected in all samples in which the corresponding gene was present
650 $aAnimal diseases
650 $aBovine mastitis
650 $aGoats
650 $aMammary gland diseases
650 $aMastitis
650 $aBovino
650 $aCaprino
650 $aDoença animal
650 $aMamite
650 $aStaphylococcus aureus
653 $aEnterodoxina estafilocócica
653 $aGenotipagem
653 $aGenotypes
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
06/04/2004 |
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Data da última atualização: |
27/07/2007 |
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Autoria: |
OLIVEIRA JUNIOR, A. de; KLEPKER, D.; BORKERT, C. M.; NOVAIS, R. F.; ALVAREZ, V. H. |
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Título: |
Availability of zinc, manganese and copper for soybean by different extraction solutions in Cerrado soils. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
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Páginas: |
p. 186. |
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Série: |
(Embrapa Soja. Documentos, 228).
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Idioma: |
Inglês |
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Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
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Conteúdo: |
Micronutrient availability can be evaluated by correlation studies between the content obtained by different soil solution extractors and the quantity of these nutrients accumulated in plants. In this manner we evaluated the efficiency of three solution extractors (Mehlich-1, Mehlich-3 e DTPA-TEA) in estimating the availability of zinc, manganese and copper for soybean in cerrado soils. The data were collected in soil calibration field experiments for micronutrients run at two sites in the region of Balsas, MA. In both sites the soil was classified as Red-yellow latosol (LVAd), but with different textures, sandy clay loam (27% of clay) and clay soil (55% of clay). Each experiment (Zn, Mn and Cu) was run for five years in a factorial arrangement (6 X 6), where six doses of lime were applied to reach base saturation between 30 to 80 %, and six doses of each micronutrient. The experimental design was in randomized blocks with four repetitions. To evaluate the efficiency of the micronutrient extraction solutions, the soil content of Zn, Mn, and Cu, were correlated with the contents of these micronutrients in soybean shoots. For zinc, DTPA-TEA was the solution extraction with the best correlation coefficient (r = 0.76) in the sandy clay loam, while in the clay soil the three solutions had similar correlation coefficients. For Mn, in both soils, DTPA-TEA was the extraction solution which best estimated the availability of Mn for soybean, when there were changes in soil pH. The other two methods, Mehlich-1 and Mehlich-3, had lower correlation coefficients. However, when the correlations were calculated inside each saturation level, the coefficients were similar, indicating that both methods might be used for estimating the availability of micronutrients when the range of base saturation or the pH of the soil is known. In the case of Cu, all the soil solution extractors evaluated were equally efficient in predicting the availability of this micronutrient to soybean, although Mehlich-1 and DTPA-TEA were slightly better than Mehlich-3. For all three micronutrients, correlation coefficients were higher in the sandy clay loam than in the clay soil. Indicating the higher readiness of the three extractors. DTPA-TEA appeared to be the most efficient method in predicting the availability of Zn, Mn and Cu for soybean in distinct soil pH conditions, although, the others methods were also efficient when the range of soil pH and of base saturation of the soil were known. MenosMicronutrient availability can be evaluated by correlation studies between the content obtained by different soil solution extractors and the quantity of these nutrients accumulated in plants. In this manner we evaluated the efficiency of three solution extractors (Mehlich-1, Mehlich-3 e DTPA-TEA) in estimating the availability of zinc, manganese and copper for soybean in cerrado soils. The data were collected in soil calibration field experiments for micronutrients run at two sites in the region of Balsas, MA. In both sites the soil was classified as Red-yellow latosol (LVAd), but with different textures, sandy clay loam (27% of clay) and clay soil (55% of clay). Each experiment (Zn, Mn and Cu) was run for five years in a factorial arrangement (6 X 6), where six doses of lime were applied to reach base saturation between 30 to 80 %, and six doses of each micronutrient. The experimental design was in randomized blocks with four repetitions. To evaluate the efficiency of the micronutrient extraction solutions, the soil content of Zn, Mn, and Cu, were correlated with the contents of these micronutrients in soybean shoots. For zinc, DTPA-TEA was the solution extraction with the best correlation coefficient (r = 0.76) in the sandy clay loam, while in the clay soil the three solutions had similar correlation coefficients. For Mn, in both soils, DTPA-TEA was the extraction solution which best estimated the availability of Mn for soybean, when there were changes in soil pH. The oth... Mostrar Tudo |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03460naa a2200205 a 4500
001 1466798
005 2007-07-27
008 2004 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA JUNIOR, A. de
245 $aAvailability of zinc, manganese and copper for soybean by different extraction solutions in Cerrado soils.
260 $c2004
300 $ap. 186.
490 $a(Embrapa Soja. Documentos, 228).
500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
520 $aMicronutrient availability can be evaluated by correlation studies between the content obtained by different soil solution extractors and the quantity of these nutrients accumulated in plants. In this manner we evaluated the efficiency of three solution extractors (Mehlich-1, Mehlich-3 e DTPA-TEA) in estimating the availability of zinc, manganese and copper for soybean in cerrado soils. The data were collected in soil calibration field experiments for micronutrients run at two sites in the region of Balsas, MA. In both sites the soil was classified as Red-yellow latosol (LVAd), but with different textures, sandy clay loam (27% of clay) and clay soil (55% of clay). Each experiment (Zn, Mn and Cu) was run for five years in a factorial arrangement (6 X 6), where six doses of lime were applied to reach base saturation between 30 to 80 %, and six doses of each micronutrient. The experimental design was in randomized blocks with four repetitions. To evaluate the efficiency of the micronutrient extraction solutions, the soil content of Zn, Mn, and Cu, were correlated with the contents of these micronutrients in soybean shoots. For zinc, DTPA-TEA was the solution extraction with the best correlation coefficient (r = 0.76) in the sandy clay loam, while in the clay soil the three solutions had similar correlation coefficients. For Mn, in both soils, DTPA-TEA was the extraction solution which best estimated the availability of Mn for soybean, when there were changes in soil pH. The other two methods, Mehlich-1 and Mehlich-3, had lower correlation coefficients. However, when the correlations were calculated inside each saturation level, the coefficients were similar, indicating that both methods might be used for estimating the availability of micronutrients when the range of base saturation or the pH of the soil is known. In the case of Cu, all the soil solution extractors evaluated were equally efficient in predicting the availability of this micronutrient to soybean, although Mehlich-1 and DTPA-TEA were slightly better than Mehlich-3. For all three micronutrients, correlation coefficients were higher in the sandy clay loam than in the clay soil. Indicating the higher readiness of the three extractors. DTPA-TEA appeared to be the most efficient method in predicting the availability of Zn, Mn and Cu for soybean in distinct soil pH conditions, although, the others methods were also efficient when the range of soil pH and of base saturation of the soil were known.
700 1 $aKLEPKER, D.
700 1 $aBORKERT, C. M.
700 1 $aNOVAIS, R. F.
700 1 $aALVAREZ, V. H.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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