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| Registros recuperados : 109 | |
| 73. |  | COSTA, P. M.; SOUZA, H. A. de; SAGRILO, E.; OLIVEIRA JUNIOR, J. O. L. de; OLIVEIRA, J. R. de; SANTOS, L. R. C. dos; PEREIRA NETO, J. A. Acúmulo e partição de massa seca do arroz de terras altas em função da calagem em áreas de abertura do Cerrado do Leste Maranhense. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA MEIO-NORTE, 8., 2022, Teresina, PI. Anais... Teresina: Embrapa Meio-Norte, 2023. p. 14. (Embrapa Meio-Norte. Documentos, 291).| Biblioteca(s): Embrapa Meio-Norte. |
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| 74. | | ARAUJO, L. G. F.; SAGRILO, E.; SOUZA, H. A. de; OLIVEIRA JUNIOR, J. O. L. de; SILVA, M. E. C. da; CLARK, M. V. G. Colonização micorrízica e seu impacto no cultivo de milho solteiro e consorciado com braquiária. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA MEIO-NORTE, 8., 2022, Teresina, PI. Anais... Teresina: Embrapa Meio-Norte, 2023. p. 30. (Embrapa Meio-Norte. Documentos, 291).| Biblioteca(s): Embrapa Meio-Norte. |
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| 75. | | MORAES, J. C. B. de; ARAÚJO, F. S.; GOMES, R. L. F.; SAGRILO, E.; ARAÚJO, A. R. de; OLIVEIRA JÚNIOR, J. O. L. de. caracterização morfo-agronômica de acessos de mandioca (manihot esculenta crantz) nas condições edafoclimáticas de Teresina, PI In: CONGRESSO BRASILEIRO DE MANDIOCA, 11., 2005, Campo Grande. Ciência e tecnologia para a raiz do Brasil: anais. Campo Grande: Governo do Estado; Dourados: Embrapa Agropecuária Oeste, 2005. 1 CD-ROM.| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 76. | | OLIVEIRA JÚNIOR, J. O. L. de; ARAÚJO, F. S.; SAGRILO, E.; SOUSA, V. F. de; LEITE, L. F. C.; BARBOSA, F. J. V. Características agronômicas de variedades tradicionais de mandioca em sistema de produção familiar no município de Iapecuru-Mirim, Maranhão In: CONGRESSO BRASILEIRO DE MANDIOCA, 11., 2005, Campo Grande. Ciência e tecnologia para a raiz do Brasil: anais. Campo Grande: Governo do Estado; Dourados: Embrapa Agropecuária Oeste, 2005. 1 CD-ROM.| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 77. | | AMORIM, I. M. de C.; ALBUQUERQUE, M. da C. C.; SOUSA, R. F. de; BARBOSA, F. J. V.; SAGRILO, E.; OLIVEIRA JÚNIOR, J. O. L. de. Níveis protéicos de genótipos de mandioca (Manihot esculenta, Crantz) no Semi-Árido Piauiense, Brasil. In: SIMPÓSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, SIRGEALC, 7, 2009, Pucón, Chile. Proceeding... Santiago de Chile: Ministério de Agricultura, Instituto de Investigaciones Agropecuarias, 2009. T. 1 p. 377-378| Biblioteca(s): Embrapa Meio-Norte. |
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| 78. | | AMORIM, I. M. de C.; ALBUQUERQUE, M. da C. C.; SOUSA, R. F. de; BARBOSA, F. J. V.; SAGRILO, E.; OLIVEIRA JÚNIOR, J. O. L. de. Níveis protéicos de genótipos de mandioca (Manihot esculenta, Crantz) no semiçarido piauense. In: SIMPÓSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, SIRGEALC, 7, 2009, Pucón, Chile. Proceeding... Santiago de Chile: Ministério de Agricultura, Instituto de Investigaciones Agropecuarias, 2009. p. 151-152 Titulo: semiçarido piauense [i. e. semi-árido piauiense].| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 109 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio-Norte; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
11/07/2019 |
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Data da última atualização: |
05/03/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SANTOS, S. E. M.; GIMENES, M. A.; OLIVEIRA JUNIOR, J. O. L. de; COSTA, M. F.; GOMES, M. F. C.; VALENTE, S. E. S. |
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Afiliação: |
MARCOS APARECIDO GIMENES, Cenargen; JOSE OSCAR LUSTOSA DE O JUNIOR, CPAMN; M. F. COSTA, UFPI; M. F. C.GOMES, UESPI; S. E. S.VALENTE, UFPI. |
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Título: |
Consequences of accelerated aging for DNA integrity and seed germination of cowpea. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 18, n. 3, article gmr18304, 2019. |
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DOI: |
10.4238/gmr18304 |
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Idioma: |
Inglês |
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Conteúdo: |
Deterioration and loss of seed germination in the germplasm are related to the aging that occurs during the conservation process. The mechanisms related to the aging and death of seeds are not yet fully understood, but it is known that longevity can be affected by moisture content and temperature. We examined the effects of accelerated aging (A.A.) on the DNA of seeds of three different cultivars of cowpea (Vigna unguiculata) and compared the data to data from vigor and germination tests. The DNA of A.A. - seeds of BR 17 Gurgueia, BRS-Marataoã and BRS-Guariba cowpea cultivars were extracted and subjected to agarose gel electrophoresis. The increase in temperature, relative humidity and time used in A.A. culminated in a progressive and increasing degradation of seed DNA, which was expressed by continuous bands of DNA molecules with different lengths in the electrophoresis gel under all but the most extreme conditions (combinations 45°C/72 h and 45°C/96 h at 100% relative humidity) under which total disintegration of the DNA band was observed. This, in turn, was reflected in a progressive decrease in seed vigor. The combination of 35ºC/48 h was the most efficient for evaluating vigor in cowpea. The BR 17 Gurgueia and BRS-Guariba cultivars showed the highest values of vigor. A lack of DNA degradation in the laddering pattern on an electrophoresis gel is an indicator that DNA degradation and cell death in cowpea seeds did not occur by apoptosis but by non-programmed processes, such as Maillard reactions, lipid peroxidation and hydrolysis of sugars. MenosDeterioration and loss of seed germination in the germplasm are related to the aging that occurs during the conservation process. The mechanisms related to the aging and death of seeds are not yet fully understood, but it is known that longevity can be affected by moisture content and temperature. We examined the effects of accelerated aging (A.A.) on the DNA of seeds of three different cultivars of cowpea (Vigna unguiculata) and compared the data to data from vigor and germination tests. The DNA of A.A. - seeds of BR 17 Gurgueia, BRS-Marataoã and BRS-Guariba cowpea cultivars were extracted and subjected to agarose gel electrophoresis. The increase in temperature, relative humidity and time used in A.A. culminated in a progressive and increasing degradation of seed DNA, which was expressed by continuous bands of DNA molecules with different lengths in the electrophoresis gel under all but the most extreme conditions (combinations 45°C/72 h and 45°C/96 h at 100% relative humidity) under which total disintegration of the DNA band was observed. This, in turn, was reflected in a progressive decrease in seed vigor. The combination of 35ºC/48 h was the most efficient for evaluating vigor in cowpea. The BR 17 Gurgueia and BRS-Guariba cultivars showed the highest values of vigor. A lack of DNA degradation in the laddering pattern on an electrophoresis gel is an indicator that DNA degradation and cell death in cowpea seeds did not occur by apoptosis but by non-programmed processe... Mostrar Tudo |
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Palavras-Chave: |
Orthodox seeds; Seed bank. |
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Thesagro: |
Vigna Unguiculata. |
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Thesaurus NAL: |
Genetic resources. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/199456/1/gmr18304-consequences-accelerated-aging-dna-integrity-and-seed-germination-cowpea.pdf
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Marc: |
LEADER 02315naa a2200241 a 4500
001 2110566
005 2020-03-05
008 2019 bl uuuu u00u1 u #d
024 7 $a10.4238/gmr18304$2DOI
100 1 $aSANTOS, S. E. M.
245 $aConsequences of accelerated aging for DNA integrity and seed germination of cowpea.$h[electronic resource]
260 $c2019
520 $aDeterioration and loss of seed germination in the germplasm are related to the aging that occurs during the conservation process. The mechanisms related to the aging and death of seeds are not yet fully understood, but it is known that longevity can be affected by moisture content and temperature. We examined the effects of accelerated aging (A.A.) on the DNA of seeds of three different cultivars of cowpea (Vigna unguiculata) and compared the data to data from vigor and germination tests. The DNA of A.A. - seeds of BR 17 Gurgueia, BRS-Marataoã and BRS-Guariba cowpea cultivars were extracted and subjected to agarose gel electrophoresis. The increase in temperature, relative humidity and time used in A.A. culminated in a progressive and increasing degradation of seed DNA, which was expressed by continuous bands of DNA molecules with different lengths in the electrophoresis gel under all but the most extreme conditions (combinations 45°C/72 h and 45°C/96 h at 100% relative humidity) under which total disintegration of the DNA band was observed. This, in turn, was reflected in a progressive decrease in seed vigor. The combination of 35ºC/48 h was the most efficient for evaluating vigor in cowpea. The BR 17 Gurgueia and BRS-Guariba cultivars showed the highest values of vigor. A lack of DNA degradation in the laddering pattern on an electrophoresis gel is an indicator that DNA degradation and cell death in cowpea seeds did not occur by apoptosis but by non-programmed processes, such as Maillard reactions, lipid peroxidation and hydrolysis of sugars.
650 $aGenetic resources
650 $aVigna Unguiculata
653 $aOrthodox seeds
653 $aSeed bank
700 1 $aGIMENES, M. A.
700 1 $aOLIVEIRA JUNIOR, J. O. L. de
700 1 $aCOSTA, M. F.
700 1 $aGOMES, M. F. C.
700 1 $aVALENTE, S. E. S.
773 $tGenetics and Molecular Research$gv. 18, n. 3, article gmr18304, 2019.
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