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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
10/08/1995 |
Data da última atualização: |
10/01/2007 |
Autoria: |
MOSCARDI, F.; OLIVEIRA, R. F. |
Afiliação: |
EMBRAPA-CNPSo. Londrina, PR. |
Título: |
Efeito de epoca de Baculovirus anticarsia sobre populacoes da lagarta da soja, Anticarsia gemmatalis. |
Ano de publicação: |
1984 |
Fonte/Imprenta: |
In: EMBRAPA. Centro Nacional de Pesquisa de Soja (Londrina, PR). Resultados de pesquisa de soja 1983/84. Londrina, 1984. |
Páginas: |
p.216-219. |
Idioma: |
Português |
Palavras-Chave: |
Aplicacao; Application; Brasil; Efeito; Effect; Epoca; Insect; Pest; Soybean; Time. |
Thesagro: |
Anticarsia Gemmatalis; Baculovirus Anticarsia; Controle Biológico; Entomologia; Inseto; Praga; Soja; Vírus. |
Thesaurus Nal: |
biological control; Brazil; entomology. |
Categoria do assunto: |
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Marc: |
LEADER 01035naa a2200385 a 4500 001 1453846 005 2007-01-10 008 1984 bl uuuu u00u1 u #d 100 1 $aMOSCARDI, F. 245 $aEfeito de epoca de Baculovirus anticarsia sobre populacoes da lagarta da soja, Anticarsia gemmatalis. 260 $c1984 300 $ap.216-219. 650 $abiological control 650 $aBrazil 650 $aentomology 650 $aAnticarsia Gemmatalis 650 $aBaculovirus Anticarsia 650 $aControle Biológico 650 $aEntomologia 650 $aInseto 650 $aPraga 650 $aSoja 650 $aVírus 653 $aAplicacao 653 $aApplication 653 $aBrasil 653 $aEfeito 653 $aEffect 653 $aEpoca 653 $aInsect 653 $aPest 653 $aSoybean 653 $aTime 700 1 $aOLIVEIRA, R. F. 773 $tIn: EMBRAPA. Centro Nacional de Pesquisa de Soja (Londrina, PR). Resultados de pesquisa de soja 1983/84. Londrina, 1984.
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Embrapa Soja (CNPSO) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
06/04/2004 |
Data da última atualização: |
27/07/2007 |
Autoria: |
ALMEIDA, A. M. R.; SAKAI, J.; HANADA, K.; OLIVEIRA, T. G.; BELINTANI, P.; KITAJIMA, E. W.; NORA, P. S.; NOVAES, T. G. |
Título: |
Biological and molecular characterization of an isolate of tobacco streak virus isolated from bud blight soybeans in Brazil. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
Páginas: |
p. 229-230. |
Série: |
(Embrapa Soja. Documentos, 228). |
Idioma: |
Inglês |
Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
Conteúdo: |
Soybean plants with symptoms of dwarfing and bud blight were observed in commercial fields near Wenceslau Braz county, State of Paraná, Brazil. The plants were used for grafting and for mechanical inoculation. Symptoms appeared 10-12 days after the inoculation or grafting and were identical to those observed in the field. Preliminary examination of leaf tissues from diseased plants by electron microscopy revealed the presence of spherical particles ca. 30 nm diameter. The host range and serological tests identified as tobacco streak virus (TSV). Purified virus showed three peaks in sucrose gradient and electron microscopy revealed spherical particles with 30 nm. Coat protein has a Mr of 29.880 kDa determined in SDS-PAGE. Polyclonal antibodies were obtained against this virus. With specific primers designed based on sequence of the TSV (Cornelissen et al., 1984 Nucleic Acids Res: 12, 277-282) a fragment of 1028 nt was RT-PCR amplified, cloned and sequenced. It contained an open reading frame with 717 nt and 238 deduced amino acids, identified and associated to the coat protein and a 3' untranslated region with 287 nucleotides. Sequence comparison of the nucleotides and predicted amino acids with sequence of TSV CP from white clover (GenBank NC003845, CAA25133) showed identities of 81% and 83%, respectively. A putative Zinc-finger motif was found in the coat protein N-terminus and the 3' untranslated region (UTR) revelead a potential stem-loop structure interpersed with AUGC-motifs, a structure which may signal binding of coat protein and activation of genome replication. The phylogenetic analysis grouped thi siolate of TSV and other TSV isolates in the same branch in 96% of the replicates.This is the first report of molecular characterization of tobacco streak virus isolated from soybeans. It is proposed that this isolate be considered a strain of TSV and named TSV-BR. MenosSoybean plants with symptoms of dwarfing and bud blight were observed in commercial fields near Wenceslau Braz county, State of Paraná, Brazil. The plants were used for grafting and for mechanical inoculation. Symptoms appeared 10-12 days after the inoculation or grafting and were identical to those observed in the field. Preliminary examination of leaf tissues from diseased plants by electron microscopy revealed the presence of spherical particles ca. 30 nm diameter. The host range and serological tests identified as tobacco streak virus (TSV). Purified virus showed three peaks in sucrose gradient and electron microscopy revealed spherical particles with 30 nm. Coat protein has a Mr of 29.880 kDa determined in SDS-PAGE. Polyclonal antibodies were obtained against this virus. With specific primers designed based on sequence of the TSV (Cornelissen et al., 1984 Nucleic Acids Res: 12, 277-282) a fragment of 1028 nt was RT-PCR amplified, cloned and sequenced. It contained an open reading frame with 717 nt and 238 deduced amino acids, identified and associated to the coat protein and a 3' untranslated region with 287 nucleotides. Sequence comparison of the nucleotides and predicted amino acids with sequence of TSV CP from white clover (GenBank NC003845, CAA25133) showed identities of 81% and 83%, respectively. A putative Zinc-finger motif was found in the coat protein N-terminus and the 3' untranslated region (UTR) revelead a potential stem-loop structure interpersed with AUGC-m... Mostrar Tudo |
Categoria do assunto: |
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Marc: |
LEADER 02968naa a2200241 a 4500 001 1466812 005 2007-07-27 008 2004 bl uuuu u00u1 u #d 100 1 $aALMEIDA, A. M. R. 245 $aBiological and molecular characterization of an isolate of tobacco streak virus isolated from bud blight soybeans in Brazil. 260 $c2004 300 $ap. 229-230. 490 $a(Embrapa Soja. Documentos, 228). 500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. 520 $aSoybean plants with symptoms of dwarfing and bud blight were observed in commercial fields near Wenceslau Braz county, State of Paraná, Brazil. The plants were used for grafting and for mechanical inoculation. Symptoms appeared 10-12 days after the inoculation or grafting and were identical to those observed in the field. Preliminary examination of leaf tissues from diseased plants by electron microscopy revealed the presence of spherical particles ca. 30 nm diameter. The host range and serological tests identified as tobacco streak virus (TSV). Purified virus showed three peaks in sucrose gradient and electron microscopy revealed spherical particles with 30 nm. Coat protein has a Mr of 29.880 kDa determined in SDS-PAGE. Polyclonal antibodies were obtained against this virus. With specific primers designed based on sequence of the TSV (Cornelissen et al., 1984 Nucleic Acids Res: 12, 277-282) a fragment of 1028 nt was RT-PCR amplified, cloned and sequenced. It contained an open reading frame with 717 nt and 238 deduced amino acids, identified and associated to the coat protein and a 3' untranslated region with 287 nucleotides. Sequence comparison of the nucleotides and predicted amino acids with sequence of TSV CP from white clover (GenBank NC003845, CAA25133) showed identities of 81% and 83%, respectively. A putative Zinc-finger motif was found in the coat protein N-terminus and the 3' untranslated region (UTR) revelead a potential stem-loop structure interpersed with AUGC-motifs, a structure which may signal binding of coat protein and activation of genome replication. The phylogenetic analysis grouped thi siolate of TSV and other TSV isolates in the same branch in 96% of the replicates.This is the first report of molecular characterization of tobacco streak virus isolated from soybeans. It is proposed that this isolate be considered a strain of TSV and named TSV-BR. 700 1 $aSAKAI, J. 700 1 $aHANADA, K. 700 1 $aOLIVEIRA, T. G. 700 1 $aBELINTANI, P. 700 1 $aKITAJIMA, E. W. 700 1 $aNORA, P. S. 700 1 $aNOVAES, T. G. 773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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