Data/hora: 24/06/2024 05:44:10

Biblioteca(s): | Embrapa Gado de Leite.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 2
Data corrente: | 07/10/2021
Data da última atualização: | 07/10/2021
Autoria: | OLIVEIRA, T. C. de A.; BRITO, M. A. P. P.; MARVAL, M. G. de; VICENTINI, N. M.; LANGE, C. C.
Afiliação: | THAIS CRISTINA DE ASSIS OLIVEIRA, Estudante, Universidade Federal de Juiz de Fora; MARIA APARECIDA VASCONCELOS PAIVA BRITO; MARCIA GIAMBIAGI DE MARVAL, Universidade Federal do Rio de Janeiro; NIVEA MARIA VICENTINI, CNPGL; CARLA CHRISTINE LANGE, CNPGL.
Título: | Identification of bovine mastitis pathogens using MALDI-TOF mass spectrometry in Brazil.
Ano de publicação: | 2021
Fonte/Imprenta: | Journal of Dairy Research, v. 88, p. 302-306, 2021.
DOI: | https://doi.org/10.1017/ S0022029921000595
Idioma: | Inglês
Conteúdo: | In this Research Communication we evaluate the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify 380 bacteria isolated from cases of bovine mastitis in Brazil. MALDI-TOF MS identifications were compared to previous identifications by biochemical tests and 16S rRNA sequencing. MALDI-TOF MS achieved a typeability of 95.5%. The accuracy of MALDI-TOF MS for the identification of Staphylococcus isolates was 93.2%. The agreement between MALDI-TOF MS and biochemical identification of Streptococcus agalactiae was 96%, however, the agreement between these techniques for identifying other catalase-negative, Gram-positive cocci was lower. Agreement in identifying Gram-negative bacteria at the genus level was 90.5%. Our findings corroborate that MALDI-TOF MS is an accurate, rapid and simple technique for identifying bovine mastitis pathogens. The availability of this methodology in some research institutions would represent a significant step toward increasing the diagnosis and epidemiological studies of bovine mastitis and other animal infectious diseases in Brazil.
Palavras-Chave: | Mastite; Staphylococci; Streptococci.
Thesagro: | Bovino; Espectrometria; Estafilococo; Estreptococo; Infecção.
Thesaurus Nal: | Bovine mastitis; Mass spectrometry.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (25431-1) - UPC - AP - DD -  -  -  - DIS