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| Registros recuperados : 162 | |
| 121. |  | OLIVEIRA, S. A. S. de; FREITAS, J. P. X. de; AUD, F. F.; SANTOS, V. da S.; OLIVEIRA, E. J. de. Resistência de genótipos s1 de mandioca (Manihot esculenta Crantz) à Mancha-Parda, queima das folhas e Mancha-Branca. In: CONGRESSO BRASILEIRO DE MANDIOCA, 15., 2013, Salvador. Inovação e sustentabilidade: da raiz ao amido: trabalhos apresentados. Salvador: CBM: Embrapa, 2013. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 123. | | VIDAL, C. B. M.; DIAMANTINO, M. S. A. S; OLIVEIRA, L. B. de; BRITO, D. A.; OLIVEIRA, S. A. S. de; FERREIRA, C. F. Enriquecimento da coleção biológica de trabalho de Xanthomonas axonopodispv. manihotis (Xam) da Embrapa Mandioca e Fruticultura: Identificação por PCR e patogenicidade. In : JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 12., 2018. Ciência profissional : resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2019. 1 p.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 124. | | REBOUÇAS, T, AM.; HADDAD, F.; FERREIRA, C. F.; OLIVEIRA, S. A. S. de; LEDO, C. A. da S.; AMORIM, E. P. Identification of banana genotypes resistant to Fusarium wilt race 1 under field and greenhouse conditions. Scientia Horticulturae, v. 239, p.308-313, 2018.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 125. | | PEREIRA, J. dos S.; ABREU, E. F. M.; DIAMANTINO, M. S. A. S.; OLIVEIRA, S. A. S. de; OLIVEIRA, E. J. de. Identificação e diagnose molecular de patógenos associados ao couro-de-sapo da mandioca (Manihot esculenta Crantz). In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 9., 2015: Cruz das Almas, BA. Pesquisa: para quê? para quem? : resumos. Brasília, DF : Embrapa, 2015.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 126. | | SILVA, A. S.; OLIVEIRA, E. J. de; HADDAD, F.; OLIVEIRA, S. A. S. de; JESUS, O. N. de; BARBOSA, F. F. L. Identificação de fontes de resistência à fusariose do maracujazeiro. Tropical Plant Pathology, v.36, n.3,Brasília, ago, 2011. Suplemento. 1 PDF. 818.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 131. | | OLIVEIRA, S. A. S. de; ABREU, E. F. M.; ARAÚJO, T. S.; OLIVEIRA, E. J. de; ANDRADE, E. C. de; GARCIA, J. M. P.; ÁLVAREZ, E. First Report of a 16SrIII-L Phytoplasma Associated with Frogskin Disease in Cassava (Manihot esculenta Crantz) in Brazil. Plant Disease, v.98, n.1, p.153, Jan., 2014. Abstract.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 135. | | OLIVEIRA, S. A. S. de; FREITAS, J. P. X. de; AUD, F. F.; SANTOS, V. da S.; OLIVEIRA, E. J. de. Avaliação da resistência de híbridos de mandioca (Manihot esculenta Crantz) À Mancha-Parda, queima das folhas e Mancha Branca. In: CONGRESSO BRASILEIRO DE MANDIOCA, 15., 2013, Salvador. Inovação e sustentabilidade: da raiz ao amido: trabalhos apresentados. Salvador: CBM: Embrapa, 2013. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 136. | | OLIVEIRA, E. J. de; KAWUKI, R.; LEDO, C. A. da S.; FERREIRA, C. F.; OLIVEIRA, S. A. S. de; SANTOS, V. da S. Avaliação da severidade do cassava mosaic disease e cassava brown streak disease em germoplasma de Manihot esculenta Crantz e outras espécies do gênero Manihot de origem americana. In: CONGRESSO BRASILEIRO DE MANDIOCA, 16.; CONGRESSO LATINO-AMERICANO E CARIBENHO DE MANDIOCA, 2015, Foz do Iguaçu. Integração: segurança alimentar e geração de renda: anais. Foz do Iguaçu: SBM, 2015. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 137. | | NASCIMENTO, D. S.; OLIVEIRA, L. B. de; BARBOSA, A. C. O.; DIAMANTINO, M. S. A. S.; FERREIRA, C. F.; OLIVEIRA, S. A. S. de. Avaliação de marcadores RAPD para estudo de diversidade de bactérias associadas ao sintoma de requeima da mandioca In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 17., 2017 Ciência e Empreendedorismo : resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2017. 137p. 1p. Biotecnologia| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 162 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
15/10/2019 |
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Data da última atualização: |
22/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
OLIVEIRA, S. A. S. de; FERREIRA, C. F.; DIAMANTINO, M. S. A. S.; SANTOS, T. A.; PEREIRA, J. dos S.; OLIVEIRA, E. J. de. |
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Afiliação: |
SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; CLAUDIA FORTES FERREIRA, CNPMF; MARIA SELMA ALVES SILVA DIAMANTINO; TAÍS ARAÚJO SANTOS, UFRB; JOCILENE DOS SANTOS PEREIRA, UFRB; EDER JORGE DE OLIVEIRA, CNPMF. |
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Título: |
First report of cassava torrado-like virus, cassava polero-like virus and cassava new alphaflexivirus associated with cassava frogskin disease in Brazil. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Plant Pathology, August, 2019. |
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ISSN: |
1125-4653 |
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DOI: |
https://doi.org/10.1007/s42161-019-00384-6 |
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Idioma: |
Inglês |
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Notas: |
Online first. |
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Conteúdo: |
Cassava (Manihot esculenta Crantz) plants with typical symptoms of frogskin disease (CFSD) have been observed since 2012 in Cruz das Almas, Bahia, Brazil. The above ground parts of the plants were asymptomatic, however, the roots showed typical symptoms of woody-like appearance, thickened cork-like peel, opaque aspect, and coalescent lip-like slits in a honeycomb pattern. Plants did not test positive for phytoplasma based on a nested-PCR reaction for the 16S region (Alvarez et al. 2009). Therefore, we hypothesized a viral infection. To verify our hypothesis, total RNA was extracted from 0.5 g of plant tissue using CTAB followed by reverse transcription and polymerase chain reaction with specific primers for: (i) cassava frogskin-associated virus ? CsFSaV; (ii) ?cassava torrado-like virus? ? CsTLV; (iii) ?cassava polero-like virus? ? CsPLV and (iv) ?cassava new alphaflexivirus? ? CsNAV, as described by Carvajal-Yepes et al. (2014). Amplicons were obtained for CsTLV (RNA1: 834 bp, RNA2: 719 bp), CsNAV (1227 bp) and CsPLV (1001 bp). No amplicons were obtained for CsFSaV. PCR products were sequenced in both directions, manually edited and deposited in the NCBI database: CsTLV (MN194209, MN194210 and MN194211) and RNA 2 (MN194212, MN194213 and MN194214), CsPLV (MN172358, MN207078 and MN207079) CsNAV (MN207080, MN207081 and MN207082). A BLASTn analysis of the viral sequences showed 96?100% identity with CsTLV (RNA1 polyprotein: KC505250), CsTLV (RNA2 polyprotein: KC505251); CsPLV (RNA-dependent RNA polymerase and coat protein: KF885738) and CsNAV (replicase, TGB1, TGB2, and coat protein: KC505252 and KY288516), respectively (E-value <0.001). The three putative virus species (CsTLV, CsNAV and CsPLV) were described for the first time by Carvajal-Yepes et al. (2014) on association with CFSD together with CsFSaV in Colombia. To our knowledge this is the first report of these three viruses associated with CFSD in Brazil. MenosCassava (Manihot esculenta Crantz) plants with typical symptoms of frogskin disease (CFSD) have been observed since 2012 in Cruz das Almas, Bahia, Brazil. The above ground parts of the plants were asymptomatic, however, the roots showed typical symptoms of woody-like appearance, thickened cork-like peel, opaque aspect, and coalescent lip-like slits in a honeycomb pattern. Plants did not test positive for phytoplasma based on a nested-PCR reaction for the 16S region (Alvarez et al. 2009). Therefore, we hypothesized a viral infection. To verify our hypothesis, total RNA was extracted from 0.5 g of plant tissue using CTAB followed by reverse transcription and polymerase chain reaction with specific primers for: (i) cassava frogskin-associated virus ? CsFSaV; (ii) ?cassava torrado-like virus? ? CsTLV; (iii) ?cassava polero-like virus? ? CsPLV and (iv) ?cassava new alphaflexivirus? ? CsNAV, as described by Carvajal-Yepes et al. (2014). Amplicons were obtained for CsTLV (RNA1: 834 bp, RNA2: 719 bp), CsNAV (1227 bp) and CsPLV (1001 bp). No amplicons were obtained for CsFSaV. PCR products were sequenced in both directions, manually edited and deposited in the NCBI database: CsTLV (MN194209, MN194210 and MN194211) and RNA 2 (MN194212, MN194213 and MN194214), CsPLV (MN172358, MN207078 and MN207079) CsNAV (MN207080, MN207081 and MN207082). A BLASTn analysis of the viral sequences showed 96?100% identity with CsTLV (RNA1 polyprotein: KC505250), CsTLV (RNA2 polyprotein: KC505251); CsPLV ... Mostrar Tudo |
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Thesagro: |
Mandioca. |
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Categoria do assunto: |
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Marc: |
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245 $aFirst report of cassava torrado-like virus, cassava polero-like virus and cassava new alphaflexivirus associated with cassava frogskin disease in Brazil.$h[electronic resource]
260 $c2019
500 $aOnline first.
520 $aCassava (Manihot esculenta Crantz) plants with typical symptoms of frogskin disease (CFSD) have been observed since 2012 in Cruz das Almas, Bahia, Brazil. The above ground parts of the plants were asymptomatic, however, the roots showed typical symptoms of woody-like appearance, thickened cork-like peel, opaque aspect, and coalescent lip-like slits in a honeycomb pattern. Plants did not test positive for phytoplasma based on a nested-PCR reaction for the 16S region (Alvarez et al. 2009). Therefore, we hypothesized a viral infection. To verify our hypothesis, total RNA was extracted from 0.5 g of plant tissue using CTAB followed by reverse transcription and polymerase chain reaction with specific primers for: (i) cassava frogskin-associated virus ? CsFSaV; (ii) ?cassava torrado-like virus? ? CsTLV; (iii) ?cassava polero-like virus? ? CsPLV and (iv) ?cassava new alphaflexivirus? ? CsNAV, as described by Carvajal-Yepes et al. (2014). Amplicons were obtained for CsTLV (RNA1: 834 bp, RNA2: 719 bp), CsNAV (1227 bp) and CsPLV (1001 bp). No amplicons were obtained for CsFSaV. PCR products were sequenced in both directions, manually edited and deposited in the NCBI database: CsTLV (MN194209, MN194210 and MN194211) and RNA 2 (MN194212, MN194213 and MN194214), CsPLV (MN172358, MN207078 and MN207079) CsNAV (MN207080, MN207081 and MN207082). A BLASTn analysis of the viral sequences showed 96?100% identity with CsTLV (RNA1 polyprotein: KC505250), CsTLV (RNA2 polyprotein: KC505251); CsPLV (RNA-dependent RNA polymerase and coat protein: KF885738) and CsNAV (replicase, TGB1, TGB2, and coat protein: KC505252 and KY288516), respectively (E-value <0.001). The three putative virus species (CsTLV, CsNAV and CsPLV) were described for the first time by Carvajal-Yepes et al. (2014) on association with CFSD together with CsFSaV in Colombia. To our knowledge this is the first report of these three viruses associated with CFSD in Brazil.
650 $aMandioca
700 1 $aFERREIRA, C. F.
700 1 $aDIAMANTINO, M. S. A. S.
700 1 $aSANTOS, T. A.
700 1 $aPEREIRA, J. dos S.
700 1 $aOLIVEIRA, E. J. de
773 $tJournal of Plant Pathology, August, 2019.
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