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| Registros recuperados : 162 | |
| 102. |  | PEREIRA, J. dos S.; DIAMANTINO, M. S. A. S.; OLIVEIRA, E. J. de; OLIVEIRA, S. A. S. de; ABREU, E. F. M. Diagnose molecular de fitoplasmas e vírus associados ao couro-desapo da mandioca (Manihot esculenta Crantz). In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 10., 2016: Cruz das Almas, BA. Traduzindo ciência para o mundo : resumos. Brasília, DF : Embrapa, 2016.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 104. | | VIDAL, C. B. M.; DIAMANTINO, M. S. A. S; OLIVEIRA, L. B. de; BRITO, D. A.; OLIVEIRA, S. A. S. de; FERREIRA, C. F. Enriquecimento da coleção biológica de trabalho de Xanthomonas axonopodispv. manihotis (Xam) da Embrapa Mandioca e Fruticultura: Identificação por PCR e patogenicidade. In : JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 12., 2018. Ciência profissional : resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2019. 1 p.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 106. | | CAVALCANTI, M. P.; BARBOSA, C. de J.; SCHNADELBACH, A. S.; BARBOSA, L. V.; HADDAD, F.; OLIVEIRA, S. A. S. de; BRAGANÇA, C. A. D. Diversidade genética de fusarium spp. associado à fusariose do maracujazeiro no Estado da Bahia por meio de marcadores SSR. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 9., 2015: Cruz das Almas, BA. Pesquisa: para quê? para quem? : resumos. Brasília, DF : Embrapa, 2015.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 107. | | CASAIS, V. O.; PATROCÍNIO, E. do; OLIVEIRA, S. A. S. de; SCHNADELBACH, A. S.; BARBOSA, C. de J.; BARBOSA, L. V. Diversidade genética de Xylella fastidiosa em regiões produtoras de citros na Bahia. Pesquisa Agropecuária Brasileira, Brasília, v.49, n.1, p.26-33, jan. 2014.| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Unidades Centrais. |
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| 108. | | OLIVEIRA, E. J. de; OLIVEIRA, S. A. S. de; MORALES, C. F. G.; SILVA, M. R.; CAMPOS, M. DE S.; OLIVEIRA FILHO, M. DOS S. BRS Progresso and BRS Rendeira - new cassava cultivars with tolerance to post-harvest deterioration. Crop Breeding and Applied Biotechnology, v. 23, n.1, p. 1-6, e444723110, 2023.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 109. | | OLIVEIRA, S. A. S. de; ABREU, E. F. M.; ARAÚJO, T. S.; OLIVEIRA, E. J. de; ANDRADE, E. C. de; GARCIA, J. M. P.; ÁLVAREZ, E. First Report of a 16SrIII-L Phytoplasma Associated with Frogskin Disease in Cassava (Manihot esculenta Crantz) in Brazil. Plant Disease, v.98, n.1, p.153, Jan., 2014. Abstract.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 112. | | RIBEIRO, L. R.; SILVA, S. de O. e; AMORIM, E. P.; SEREJO, J. A. dos S.; OLIVEIRA, S. A. S. de; HADDAD, F. Avaliação de germoplasma de bananeira quanto a resistência ao Mal-do-Panamá Evaluation germplasm of the banana to resistance to panama disease. In: REUNIÃO INTERNACIONAL ACORBAT, 20., 2013, Fortaleza. Acorbat: 40 anos compartilhando ciência e tecnologia. Fortaleza: Instituto Frutal: Acorbat Internacional, 2013. 320 p.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 113. | | GUIMARÃES, A. L. de S.; MATOS, A. P. de; CORDEIRO, Z. J. M.; OLIVEIRA, S. A. S. de; AMORIM, E. P.; HADDAD, F. Avaliação de genótipos de bananeira quanto a resistência ao mal-do-Panamá em condições de campo. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 6., 2012, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura, 2012.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 114. | | GUIMARÃES, Â. L. de S.; MATOS, A. P. de; CORDEIRO, Z. J. M.; OLIVEIRA, S. A. S. de; AMORIM, E. P.; HADDAD, F. Avaliação de genótipos de bananeira quanto à resistência ao mal-do-Panamá em condições de campo. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA TROPICAL, 7., 2013, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura, 2013. Publicação online.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 115. | | NASCIMENTO, D. S.; OLIVEIRA, L. B. de; BARBOSA, A. C. O.; DIAMANTINO, M. S. A. S.; FERREIRA, C. F.; OLIVEIRA, S. A. S. de. Avaliação de marcadores RAPD para estudo de diversidade de bactérias associadas ao sintoma de requeima da mandioca In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 17., 2017 Ciência e Empreendedorismo : resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2017. 137p. 1p. Biotecnologia| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 116. | | OLIVEIRA, S. A. S. de; FREITAS, J. P. X. de; AUD, F. F.; SANTOS, V. da S.; OLIVEIRA, E. J. de. Avaliação da resistência de híbridos de mandioca (Manihot esculenta Crantz) À Mancha-Parda, queima das folhas e Mancha Branca. In: CONGRESSO BRASILEIRO DE MANDIOCA, 15., 2013, Salvador. Inovação e sustentabilidade: da raiz ao amido: trabalhos apresentados. Salvador: CBM: Embrapa, 2013. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 117. | | HOHENFELD, C. S.; VILDOSO, C. I. A.; SANTOS, N. S. da C. dos; OLIVEIRA, E. J. de; HADDAD, F.; OLIVEIRA, S. A. S. de. Avaliação de acessos do BAG-Mandioca quanto a resistência à Fusarium sp. em ambiente controlado. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 6., 2012, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura, 2012.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 119. | | OLIVEIRA, E. J. de; KAWUKI, R.; LEDO, C. A. da S.; FERREIRA, C. F.; OLIVEIRA, S. A. S. de; SANTOS, V. da S. Avaliação da severidade do cassava mosaic disease e cassava brown streak disease em germoplasma de Manihot esculenta Crantz e outras espécies do gênero Manihot de origem americana. In: CONGRESSO BRASILEIRO DE MANDIOCA, 16.; CONGRESSO LATINO-AMERICANO E CARIBENHO DE MANDIOCA, 2015, Foz do Iguaçu. Integração: segurança alimentar e geração de renda: anais. Foz do Iguaçu: SBM, 2015. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 162 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
06/11/2017 |
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Data da última atualização: |
06/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CARVALHO, M. J. S.; OLIVEIRA, E. J. de; SOUZA, A. da S.; PEREIRA, J. S.; DIAMANTINO, M. S. A. S.; OLIVEIRA, S. A. S. de. |
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Afiliação: |
M. J. S. CARVALHO; EDER JORGE DE OLIVEIRA, CNPMF; ANTONIO DA SILVA SOUZA, CNPMF; J. S. PEREIRA; M. S .A. S. DIAMANTINO; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF. |
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Título: |
Cleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 16, n.2, 2017. |
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Série: |
1676-5680 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus - CsCMV (by ELISA) and Cassava vein mosaic virus - CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV. Subsequently, the cuttings were submitted to different tetracycline concentrations for 3 min, and then subjected to thermotherapy under different temperatures (35°, 38°, 40°, 45°, and 55°C). Shoots of 2 cm were harvested, and their surfaces were sterilized in a laminar flow chamber. Subsequently, the shoot tips of different sizes were removed (0.2, 0.4, 0.5, and 1.0 mm) for inoculation in a culture medium with tetracycline at the same concentrations in which the cuttings were dipped. After 60 days of cultivation, the explants were transferred to a multiplication medium without antibiotics. Thirty days after the transfer, the viability of the regenerated plants was evaluated, which were then acclimatized for 70 days in a greenhouse and transferred to the field. After 7 months, a visual analysis of the symptomatic roots and a PCR analysis were held to prove the elimination of CFSD and CsVMV from the accessions infected with these viruses (BGM0315 and BGM0464), respectively. Most of the treatments resulted in 100% cleaning of CFSD-infected plants. From accessions that were also infected with CsVMV, only 2% of the plants remained infected, also demonstrating the cleaning efficiency of this protocol for this disease. MenosThis study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus - CsCMV (by ELISA) and Cassava vein mosaic virus - CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV. Subsequently, the cuttings were submitted to different tetracycline concentrations for 3 min, and then subjected to thermotherapy under different temperatures (35°, 38°, 40°, 45°, and 55°C). Shoots of 2 cm were harvested, and their surfaces were sterilized in a laminar flow chamber. Subsequently, the shoot tips of different sizes were removed (0.2, 0.4, 0.5, and 1.0 mm) for inoculation in a culture medium with tetracycline at the same concentrations in which the cuttings were dipped. After 60 days of cultivation, the explants were transferred to a multiplication medium without antibiotics. Thirty days after the transfer, the viability of the regenerated plants was evaluated, which were then acclimatized for 70 days in a greenhouse and transferred to the field. After 7 months, a visual analysis of the symptomatic ro... Mostrar Tudo |
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Thesagro: |
Mandioca. |
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Thesaurus NAL: |
cassava. |
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Categoria do assunto: |
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Marc: |
LEADER 02559naa a2200217 a 4500
001 2078909
005 2017-11-06
008 2017 bl --- 0-- u #d
100 1 $aCARVALHO, M. J. S.
245 $aCleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture.$h[electronic resource]
260 $c2017
490 $a1676-5680
520 $aThis study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus - CsCMV (by ELISA) and Cassava vein mosaic virus - CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV. Subsequently, the cuttings were submitted to different tetracycline concentrations for 3 min, and then subjected to thermotherapy under different temperatures (35°, 38°, 40°, 45°, and 55°C). Shoots of 2 cm were harvested, and their surfaces were sterilized in a laminar flow chamber. Subsequently, the shoot tips of different sizes were removed (0.2, 0.4, 0.5, and 1.0 mm) for inoculation in a culture medium with tetracycline at the same concentrations in which the cuttings were dipped. After 60 days of cultivation, the explants were transferred to a multiplication medium without antibiotics. Thirty days after the transfer, the viability of the regenerated plants was evaluated, which were then acclimatized for 70 days in a greenhouse and transferred to the field. After 7 months, a visual analysis of the symptomatic roots and a PCR analysis were held to prove the elimination of CFSD and CsVMV from the accessions infected with these viruses (BGM0315 and BGM0464), respectively. Most of the treatments resulted in 100% cleaning of CFSD-infected plants. From accessions that were also infected with CsVMV, only 2% of the plants remained infected, also demonstrating the cleaning efficiency of this protocol for this disease.
650 $acassava
650 $aMandioca
700 1 $aOLIVEIRA, E. J. de
700 1 $aSOUZA, A. da S.
700 1 $aPEREIRA, J. S.
700 1 $aDIAMANTINO, M. S. A. S.
700 1 $aOLIVEIRA, S. A. S. de
773 $tGenetics and Molecular Research$gv. 16, n.2, 2017.
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