Data/hora: 24/06/2024 22:01:31

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 19/09/2018
Data da última atualização: | 28/09/2018
Autoria: | SILVA, L. L.; PESTANA, K. N.; FERREIRA, C. F.; OLIVEIRA, S. A. S. de.
Afiliação: | LEANDRO L. SILVA, UFRB; KÁTIA N. PESTANA; CLAUDIA FORTES FERREIRA, CNPMF; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF.
Título: | Differentiation of phylogenetic lineages within the 'Colletotrichum gloeosporioides species complex' associated with cassava anthracnose disease by PCR-RFLP.
Ano de publicação: | 2018
Fonte/Imprenta: | Tropical Plant Pathology, v.43, p.194-20, 2018.
ISSN: | 1983-2052
Idioma: | Inglês
Conteúdo: | Different species were found associated with cassava anthracnose, considered a major disease of this crop. The correct identification of the causal agent is a first step for defining appropriate control strategies, such as resistant varieties. In silico analyses used sequences of six genomic regions of ex-type specimens from the ?Colletotrichum gloeosporioides species complex? (C.g.SC) and 21 restriction enzymes to identify phylogenetic lineages. The three best combinations of region/enzymes were validated in 18 Colletotrichum spp. isolates from cassava. Dendrograms for in silico PCR-RFLP for CAL, ITS and TUB2 showed considerable agreement with the phylogenetic analysis of each genomic region; however, the CAL gene presented greater discriminatory power. Since the band patterns from in gel analysis were almost the same as expected for the in silico analysis for the CAL region, a new approach was proposed based on the combined data from these two methodologies, allowing the differentiation of five phylogenetic lineages within the C.g.SC (C. tropicale; C. fructicola; C. siamense; C. gloeosporioides sensu stricto and C. theobromicola), and one outside of this complex (C. cliviae). This evaluation showed to be a reliable technique for preliminary identification of species prior to sequencing.
Thesagro: | Mandioca.
Thesaurus Nal: | Cassava.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (32498-1) - UPC - AP - DD - Publicação digital -  -  - DIS