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 | Acesso ao texto completo restrito à biblioteca da Embrapa Tabuleiros Costeiros. Para informações adicionais entre em contato com cpatc.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
12/09/2016 |
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Data da última atualização: |
13/04/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SA, F. P. de; LEDO, A. da S.; AMORIM, J. A. E.; MUNIZ, A. V. C. da S.; PASQUAL, M. |
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Afiliação: |
FRANCIELEN PAOLA DE SA; ANA DA SILVA LEDO, CPATC; JULIE ANNE ESPINDOLA AMORIM; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; MOACIR PASCOAL. |
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Título: |
In vitro propagation and acclimatization of genipapo accessions. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Ciência e Agrotecnologia, Lavras, MG, v. 40, n. 2, p. 155-163, mar./abr. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Genipapo is a species with an intermediate seed storage behavior, and its germination is slow and asynchronous; therefore the tissue culture is an alternative strategy for the propagation in large-scale. This study aimed to evaluate three genipapo accessions at different micropropagation stages (adventitious shoot induction, in vitro rooting and acclimatization). Genipapo nodal segments from the Sabinópolis (AS), Cruz das Almas (CZA) and Núcleo Bandeirante (NB) accessions were used and inoculated in culture media with 0 and 1.0 mg L-1 6-benzylaminopurine (BAP). Different indole-3-butyric acid (IBA) concentrations were tested for in vitro rooting. Subsequently, the effect of two substrates on the seedling development of three genipapo accessions was assessed. The molecular characterization of accessions was performed using 15 inter-simple sequence repeat (ISSR) primers, and genotype clustering was performed based on genetic dissimilarity using the Unweighted Pair-Group Method with Arithmetic Mean (UPGMA). Culture media supplementation with 1.0 mg L-1 BAP favors the development of adventitious shoots and promotes a higher callogenesis percentage, leaf number and feasibility at 90 days of in vitro culture. The immersion of the basal portion of shoots for 20 seconds in all IBA solutions induces the rhizogenesis in the genipapo accessions at 90 days of in vitro culture. The substrates consisting of washed sand + dried coconut shell powder, at a 1:1 ratio by volume, and Topstrato HT® may be recommended for genipapo acclimatization. The NB accession is genetically different from the CZA and AS populations and shows superiority for most of the variables analyzed. MenosGenipapo is a species with an intermediate seed storage behavior, and its germination is slow and asynchronous; therefore the tissue culture is an alternative strategy for the propagation in large-scale. This study aimed to evaluate three genipapo accessions at different micropropagation stages (adventitious shoot induction, in vitro rooting and acclimatization). Genipapo nodal segments from the Sabinópolis (AS), Cruz das Almas (CZA) and Núcleo Bandeirante (NB) accessions were used and inoculated in culture media with 0 and 1.0 mg L-1 6-benzylaminopurine (BAP). Different indole-3-butyric acid (IBA) concentrations were tested for in vitro rooting. Subsequently, the effect of two substrates on the seedling development of three genipapo accessions was assessed. The molecular characterization of accessions was performed using 15 inter-simple sequence repeat (ISSR) primers, and genotype clustering was performed based on genetic dissimilarity using the Unweighted Pair-Group Method with Arithmetic Mean (UPGMA). Culture media supplementation with 1.0 mg L-1 BAP favors the development of adventitious shoots and promotes a higher callogenesis percentage, leaf number and feasibility at 90 days of in vitro culture. The immersion of the basal portion of shoots for 20 seconds in all IBA solutions induces the rhizogenesis in the genipapo accessions at 90 days of in vitro culture. The substrates consisting of washed sand + dried coconut shell powder, at a 1:1 ratio by volume, and Topstrato ... Mostrar Tudo |
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Palavras-Chave: |
Genética de planta; Genipapo. |
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Thesagro: |
Fruta tropical; Jenipapo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02322naa a2200217 a 4500
001 2052710
005 2017-04-13
008 2016 bl uuuu u00u1 u #d
100 1 $aSA, F. P. de
245 $aIn vitro propagation and acclimatization of genipapo accessions.$h[electronic resource]
260 $c2016
520 $aGenipapo is a species with an intermediate seed storage behavior, and its germination is slow and asynchronous; therefore the tissue culture is an alternative strategy for the propagation in large-scale. This study aimed to evaluate three genipapo accessions at different micropropagation stages (adventitious shoot induction, in vitro rooting and acclimatization). Genipapo nodal segments from the Sabinópolis (AS), Cruz das Almas (CZA) and Núcleo Bandeirante (NB) accessions were used and inoculated in culture media with 0 and 1.0 mg L-1 6-benzylaminopurine (BAP). Different indole-3-butyric acid (IBA) concentrations were tested for in vitro rooting. Subsequently, the effect of two substrates on the seedling development of three genipapo accessions was assessed. The molecular characterization of accessions was performed using 15 inter-simple sequence repeat (ISSR) primers, and genotype clustering was performed based on genetic dissimilarity using the Unweighted Pair-Group Method with Arithmetic Mean (UPGMA). Culture media supplementation with 1.0 mg L-1 BAP favors the development of adventitious shoots and promotes a higher callogenesis percentage, leaf number and feasibility at 90 days of in vitro culture. The immersion of the basal portion of shoots for 20 seconds in all IBA solutions induces the rhizogenesis in the genipapo accessions at 90 days of in vitro culture. The substrates consisting of washed sand + dried coconut shell powder, at a 1:1 ratio by volume, and Topstrato HT® may be recommended for genipapo acclimatization. The NB accession is genetically different from the CZA and AS populations and shows superiority for most of the variables analyzed.
650 $aFruta tropical
650 $aJenipapo
653 $aGenética de planta
653 $aGenipapo
700 1 $aLEDO, A. da S.
700 1 $aAMORIM, J. A. E.
700 1 $aMUNIZ, A. V. C. da S.
700 1 $aPASQUAL, M.
773 $tCiência e Agrotecnologia, Lavras, MG$gv. 40, n. 2, p. 155-163, mar./abr. 2016.
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Embrapa Tabuleiros Costeiros (CPATC) |
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| Registros recuperados : 4 | |
| 1. |  | BRAGA, L. D. da C.; PRAXEDES, V. A.; BRITO, L. F.; PEIXOTO, M. G. C. D.; OLIVEIRA, M. M. N. F. de; PIRES, M. de F. A. Comportamento materno-filial de vacas Guzerá com bezerros mestiços Holandês x Guzerá em rebanhos leiteiros. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 6.; SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 7.; FÓRUM DE COORDENADORES DE PÓS GRADUAÇÃO EM PRODUÇÃO ANIMAL DO NORDESTE, 1.; FÓRUM DE AGROECOLOGIA RO RIO GRANDE DO NORTE, 1., 2010, Mossoró. Anais... Mossoró: Sociedade Nordestina de Producao Animal; UFERSA, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 2. | | BRAGA, L. D. da C.; PRAXEDES, V. A.; BRITO, L. F.; PEIXOTO, M. G. C. D.; OLIVEIRA, M. M. N. F. de; PIRES, M. de F. A. Influência da presença do urubu (Coragyps atratus) na interação materno filial após o parto. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 6.; SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 7.; FÓRUM DE COORDENADORES DE PÓS GRADUAÇÃO EM PRODUÇÃO ANIMAL DO NORDESTE, 1.; FÓRUM DE AGROECOLOGIA RO RIO GRANDE DO NORTE, 1., 2010, Mossoró. Anais... Mossoró: Sociedade Nordestina de Producao Animal; UFERSA, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | AMORIM, L. da S.; TORRES, C. A. A.; SIQUEIRA, L. G. B.; FONSECA, J. F. da; GUIMARÃES, J. D.; CARVALHO, G. R. de; ALVES, N. G.; OLIVEIRA, M. M. N. F. de. Embryo development and follicular status of Toggenburg does fed urea diet. Revista Brasileira de Zootecnia, v. 40, n. 2, p. 277-285, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| 4. | | NASCIMENTO, V. A.; TORRES, C. A. A.; OLIVEIRA, M. M. N. F. de; LOGRADO, M. L.; VIANA, J. H. M.; COSTA, E. P. da; SOUSA, K. R. S.; CARNEIRO, C.; OLIVEIRA, F. A. Oestrous and of the ovulation synchronization, artificial insemination in fixed time and pregnancy values in nelore milk cows. Acta Scientiae Veterinariae, v. 34, s282, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 4 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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