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| Registros recuperados : 202 | |
| 81. |  | LIMA, S. C. D. V.; WENDLAND, A.; OLIVEIRA, M. I. D. S.; MELO, L. C.; PEREIRA, H. S.; SANTOS, P. G.; ARAUJO, L. G. Agressividade de dois isolados de Curtobacterium flaccumfaciens pv. flaccumfaciens em 41 genótipos de feijoeiro. Tropical Plant Pathology, Brasília, DF, v. 38, p. 118, ago. 2013. Suplemento. ref. 179-1. Edição dos Resumos do XLVI Congresso Brasileiro de Fitopatologia, Ouro Preto, MG, ago. 2013.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 82. | | LIMA, S. C. D. V.; WENDLAND, A.; OLIVEIRA, M. I. S.; ARAÚJO, L. G. de; MELO, L. C.; PEREIRA, H. S.; FERREIRA, E. P. de B. Agressividade de isolados de curtobacterium flaccumfaciens pv. flaccumfaciens em feijoeiro comum. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 10., 2011, Goiânia. Anais... Goiânia: Embrapa Arroz e Feijão, 2011. 1 CD-ROM. CONAFE.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 83. | | BEZERRA, G. de A.; OLIVEIRA, M. I. de S.; COSTA, N. B.; ELIAS, M. T. A.; ARRIEL, G. C. T. F.; FILIPPI, M. C. C. de. Antagonismo in vitro e in vivo de Phanerochaete australis a Bipolaris oryzae. In: SEMINÁRIO JOVENS TALENTOS, 14., 2020, Santo Antônio de Goiás. Resumos... Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2021. p. 66.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 84. | | SANTOS, D. S. dos; MAJOLO, C.; SANTOS, W. B. dos; OLIVEIRA, M. I. B. de; FARIAS, C. F. S.; BRANDÃO, F. R.; CHAGAS, E. C. Anthelmintic activity of eugenol, tannin and thymol against Neoechinorhynchus buttnerae. Archives of Veterinary Science, v. 26, n. 4, p. 117-127, 2021. Título em Português: Atividade anti-helmíntica de eugenol, tanino e timol frente à Neoechinorhynchus buttnerae.| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 85. | | OLIVEIRA, M. I. L. de; BRUNET, D.; MITJA, D.; CARDOSO, W. dos S.; BENITO, N. P.; GUIMARÃES, M. de F.; BROSSARD, M. Incidence of epigeal nest-building termites in Brachiaria pastures in the Cerrado. Acta Scientiarum. Agronom, v. 33, n. 1, p. 181-185, 2011| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 86. | | BRAGA, F. C. S.; LEITE, A. A. da S.; ANGÉLICA, R. S.; MARTINS, E. de S.; OLIVEIRA, M. I. L. de; WENDER, I. G. K-alternative fertilizer project: utrapotassic syenite deposit in Pernambuco State. In: WORLD FERTILIZER CONGRESS OF CIEC, 16., 2014, Rio de Janeiro. Technological innovation for a sustainable tropical agriculture: proceedings. Rio de Janeiro: International Scientific Centre of Fertilizers, 2014. p. 167-169.| Biblioteca(s): Embrapa Cerrados. |
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| 87. | | OLIVEIRA, M. A. de; LEITE, A. A. da S.; NOVAES, L. A. A.; ANGÉLICA, R. S.; OLIVEIRA, M. I. L. de; MARTINS, E. de S. K, Ca and Mg-bearing agrominerals from ultramafic rocks: soil conditioners and k-fertilizers source. In: WORLD FERTILIZER CONGRESS OF CIEC, 16., 2014, Rio de Janeiro. Technological innovation for a sustainable tropical agriculture: proceedings. Rio de Janeiro: International Scientific Centre of Fertilizers, 2014. p. 170-172.| Biblioteca(s): Embrapa Cerrados. |
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| 88. | | ARRIEL, M. T.; OLIVEIRA, M. I. de S.; CORTES, M. V. de C. B.; BARBOSA, E. T.; FILIPPI, M. C. C. de. Investigando as diferentes formas de aplicação das bactérias Pseudomonas fluorescens e Burkholderia pyrrocinia na eficiência da supressão da brusone do arroz. In: SEMINÁRIO JOVENS TALENTOS, 11., 2017, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2017. p. 33. (Embrapa Arroz e Feijão. Documentos, 316).| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 89. | | MENDES, I. de C.; BORIN, A. L. D. C.; OLIVEIRA, M. I. L. de; REIS JUNIOR, F. B. dos; CHAER, G. M. Diagnóstico da saúde do solo nas áreas de produção de algodão no Estado de Goiás. In: CONGRESSO BRASILEIRO DO ALGODÃO, 12., 2019, Goiânia, GO. Anais... Goiânia: Abrapa, 2019.| Biblioteca(s): Embrapa Algodão. |
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| 90. | | MENDES, I. de C.; BORIN, A. L. D. C.; OLIVEIRA, M. I. L. de; REIS JUNIOR, F. B. dos; CHAER, G. M. Diagnóstico da saúde do solo nas áreas de produção de algodão no Estado de Goiás. In: CONGRESSO BRASILEIRO DO ALGODÃO, 12., 2019, Goiânia, GO. Anais... Goiânia: Abrapa, 2019.| Biblioteca(s): Embrapa Agrobiologia. |
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| 91. | | SEBASTIÃO, F. de A.; OLIVEIRA, M. I. B. de; ROCHA, M. J. S.; SOUZA, D. C. de M.; RIBEIRO, P.; MAJOLO, C.; CRESCENCIO, R.; CHAGAS, E. C. Effect of a food additive in the control of the acanthocephalan Neoechinorhynchus buttnerae in Colossoma macropomum. Aquaculture Research, v. 52, n. 2, p. 635-642, Feb. 2021.| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 92. | | WENDLAND, A.; MODA-CIRINO, V.; DEL PELOSO, M. J.; COSTA, J. G. C.; OLIVEIRA, M. I. S.; LIMA, S. C. D. V.; ARAÚJO, L. G. Murcha-de-curtobacterium. In: PAULA JÚNIOR, T. J. de; WENDLAND, A. (Ed.). Melhoramento genético do feijoeiro-comum e prevenção de doenças. Viçosa, MG: Epamig, 2012. p. 111-125.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 93. | | BELTRAO, N. E. de M.; OLIVEIRA, M. I. P. de; LUCENA, A. M. A. de; SANTOS, J. W. dos; SOUZA, J. G. de. Modificações no algodoeiro herbáceo superprecoce sob influência do cloreto de Mepiquat. Revista Brasileira de Oleaginosas e Fibrosas, v. 14, n. 1, p. 29-35, jan./abr., 2010.| Biblioteca(s): Embrapa Algodão. |
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| 94. | | BELTRAO, N. E. de M.; OLIVEIRA, M. I. P. de; BRITO NETO, J. F. de; SOFIATTI, V.; FRANÇA, F. V. de. Modificações no crescimento do algodoeiro herbáceo causadas pela temperatura do ambiente. In: CONGRESSO BRASILEIRO DO ALGODÃO, 8.; COTTON EXPO, 1., 2011, São Paulo. Evolução da cadeia para construção de um setor forte: Anais. Campina Grande, PB: Embrapa Algodão, 2011. p.1053-1060| Biblioteca(s): Embrapa Algodão. |
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| 95. | | OLIVEIRA, M. I. P. de; FREIRE, M. A. de O.; SAMPAIO, L. S.; ALBUQUERQUE, W. G. de; RODRIGUES, D. de M.; BELTRÃO, N. E. de M. Crescimento inicial de Pinhão Manso oriundas de sementes tratadas com Cloreto de Mepiquat. In: CONGRESSO BRASILEIRO DE MAMONA, 3., 2008, Salvador. Energia e ricinoquímica: resumos. Salvador: SEAGRI: Embrapa Algodão, 2008. p. 114| Biblioteca(s): Embrapa Algodão. |
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| 96. | | OLIVEIRA, M. I. P. de; FREIRE, M. A. de O.; SAMPAIO, L. S.; ALBUQUERQUE, W. G. de; RODRIGUES, D. de M.; BELTRÃO, N. E. de M. Crescimento inicial de Pinhão Manso oriundas de sementes tratadas com Cloreto de Mepiquat. In: CONGRESSO BRASILEIRO DE MAMONA, 3., 2008, Salvador. Energia e ricinoquímica: resumos. Salvador: SEAGRI: Embrapa Algodão, 2008. p. 114| Biblioteca(s): Embrapa Rondônia. |
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| 97. | | OLIVEIRA, M. I. P. de; FREIRE, M. A. de O.; SAMPAIO, L. S.; ALBUQUERQUE, W. G. de; RODRIGUES, D. de M.; BELTRÃO, N. E. de M. Crescimento inicial de Pinhão Manso oriundas de sementes tratadas com Cloreto de Mepiquat. In: CONGRESSO BRASILEIRO DE MAMONA, 3., 2008, Salvador. Energia e ricinoquímica: anais. Salvador: SEAGRI: Embrapa Algodão, 2008. 1 CD-ROM| Biblioteca(s): Embrapa Algodão. |
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| 99. | | TEIXEIRA, N. C.; WENDLAND, A.; DUARTE, L. T.; OLIVEIRA, M. I. S.; SOUZA, T. L. P. O.; FARIA, J. C.; CORTES, M. V. B. Desenvolvimento de Loop-mediated isothermal amplification para detecção de feijão transgênico visualmente interpretada pela adição de corantes indicadores de pH. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 12., 2017, Piracicaba. Produtividade e sustentabilidade da cultura do feijão: do campo para a mesa: resumos. Piracicaba: CENA: IAC, 2017. p. 25. CONAFE| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 202 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
06/11/2020 |
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Data da última atualização: |
30/04/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CÔRTES, M. V. de C. B.; OLIVEIRA, M. I. de S.; MATEUS, J. R.; SELDIN, L.; SILVA-LOBO, V. L.; FREIRE, D. M. G. |
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Afiliação: |
MARCIO VINICIUS DE C BARROS CORTES, CNPAF; MAYTHSULENE INACIO DE SOUSA OLIVEIRA, bolsista CNPAF; JACKELINE ROSSETTI MATEUS, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; LUCY SELDIN, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; VALACIA LEMES DA SILVA LOBO, CNPAF; DENISE MARIA GUIMARAES FREIRE, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO. |
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Título: |
A pipeline for the genetic improvement of a biological control agent enhances its potential for controlling soil-borne plant pathogens. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Biological Control, v. 152, 104460, 2021. |
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ISSN: |
1049-9644 |
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DOI: |
https://doi.org/10.1016/j.biocontrol.2020.104460 |
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Idioma: |
Inglês |
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Conteúdo: |
Use of biopesticides results in a reduction of the worldwide dependence on chemical pesticides, ending in a more sustainable crop protection. For this reason, biological control industry invests in continued research for more effective biocontrol agents (BCAs) using basically two strategies: isolation of new strains from nature or conventional genetic improvement. However, both are highly onerous and time-consuming. Here we show a pipeline for the genetic improvement of fungal BCAs, based on genome shuffling, the most recent and promising non-recombinant DNA technology for the rapid phenotype improvement of microbial strains. The method consisted of the construction of a parent library using mutagenic agents, followed by genome shuffling and high-throughput screening. Sarocladium oryzae BRM 6461, a known cerulenin antifungal producer strain and high biocontrol potential, served as the model fungal BCA. The pipeline aimed to select mutant enhanced strains at least one of these three desirable characteristics: antagonism, UV-B irradiation tolerance and high temperature tolerance. The experiments were conducted in laboratory and green house. After four cycles of genome shuffling we selected the superior S. oryzae GS4-03 strain, showing cerulenin production of 203.3 ± 1.4 µg/mL, 42% higher than the wild-type strain BRM 6461. The GS4-03 strain exhibited good genetic stability for at least five successive subcultivation assays. Antagonism assay showed an increased micelial inhibition of Rhizoctonia solani and Sclerotinia sclerotiorum when using GS4-03 strain compared to the BRM 6461 strain. The bioassays, showed that the superior strain GS4-03 have an increased ability to control root rot (23.4%) and white mold (8.1 times) disease compared to the wild type strain BRM 6461. Assessment of virulence of the superior strain GS4-03 against rice plants showed that improvement procedures did not change the original strain behavior in this subject. Hydrolytic activity of enzymes related to the biocontrol action as chitinase, protease and B-1,3-glucanase did not present statistical difference between GS4-03 and BRM 6461 strains. Results of dual assay culture between GS4-03 and BRM 6461 strains showed that both have different genetic backgrounds, although RAPD and ITS-rDNA tests were not fully efficient to distinguish genetically the strains. Moreover, mutations resulted in S. oryzae strains more tolerant to UV-B irradiation, but with low genetic stability. Our results report for the first time an efficient pipeline for the genetic improvement of fungal BCAs based on genome shuffling. MenosUse of biopesticides results in a reduction of the worldwide dependence on chemical pesticides, ending in a more sustainable crop protection. For this reason, biological control industry invests in continued research for more effective biocontrol agents (BCAs) using basically two strategies: isolation of new strains from nature or conventional genetic improvement. However, both are highly onerous and time-consuming. Here we show a pipeline for the genetic improvement of fungal BCAs, based on genome shuffling, the most recent and promising non-recombinant DNA technology for the rapid phenotype improvement of microbial strains. The method consisted of the construction of a parent library using mutagenic agents, followed by genome shuffling and high-throughput screening. Sarocladium oryzae BRM 6461, a known cerulenin antifungal producer strain and high biocontrol potential, served as the model fungal BCA. The pipeline aimed to select mutant enhanced strains at least one of these three desirable characteristics: antagonism, UV-B irradiation tolerance and high temperature tolerance. The experiments were conducted in laboratory and green house. After four cycles of genome shuffling we selected the superior S. oryzae GS4-03 strain, showing cerulenin production of 203.3 ± 1.4 µg/mL, 42% higher than the wild-type strain BRM 6461. The GS4-03 strain exhibited good genetic stability for at least five successive subcultivation assays. Antagonism assay showed an increased micelial inhibit... Mostrar Tudo |
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Thesagro: |
Controle Biológico; Mofo Branco; Patógeno. |
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Thesaurus NAL: |
Biological control; Genetics; Genome; Root rot; Sarocladium oryzae. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
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022 $a1049-9644
024 7 $ahttps://doi.org/10.1016/j.biocontrol.2020.104460$2DOI
100 1 $aCÔRTES, M. V. de C. B.
245 $aA pipeline for the genetic improvement of a biological control agent enhances its potential for controlling soil-borne plant pathogens.$h[electronic resource]
260 $c2021
520 $aUse of biopesticides results in a reduction of the worldwide dependence on chemical pesticides, ending in a more sustainable crop protection. For this reason, biological control industry invests in continued research for more effective biocontrol agents (BCAs) using basically two strategies: isolation of new strains from nature or conventional genetic improvement. However, both are highly onerous and time-consuming. Here we show a pipeline for the genetic improvement of fungal BCAs, based on genome shuffling, the most recent and promising non-recombinant DNA technology for the rapid phenotype improvement of microbial strains. The method consisted of the construction of a parent library using mutagenic agents, followed by genome shuffling and high-throughput screening. Sarocladium oryzae BRM 6461, a known cerulenin antifungal producer strain and high biocontrol potential, served as the model fungal BCA. The pipeline aimed to select mutant enhanced strains at least one of these three desirable characteristics: antagonism, UV-B irradiation tolerance and high temperature tolerance. The experiments were conducted in laboratory and green house. After four cycles of genome shuffling we selected the superior S. oryzae GS4-03 strain, showing cerulenin production of 203.3 ± 1.4 µg/mL, 42% higher than the wild-type strain BRM 6461. The GS4-03 strain exhibited good genetic stability for at least five successive subcultivation assays. Antagonism assay showed an increased micelial inhibition of Rhizoctonia solani and Sclerotinia sclerotiorum when using GS4-03 strain compared to the BRM 6461 strain. The bioassays, showed that the superior strain GS4-03 have an increased ability to control root rot (23.4%) and white mold (8.1 times) disease compared to the wild type strain BRM 6461. Assessment of virulence of the superior strain GS4-03 against rice plants showed that improvement procedures did not change the original strain behavior in this subject. Hydrolytic activity of enzymes related to the biocontrol action as chitinase, protease and B-1,3-glucanase did not present statistical difference between GS4-03 and BRM 6461 strains. Results of dual assay culture between GS4-03 and BRM 6461 strains showed that both have different genetic backgrounds, although RAPD and ITS-rDNA tests were not fully efficient to distinguish genetically the strains. Moreover, mutations resulted in S. oryzae strains more tolerant to UV-B irradiation, but with low genetic stability. Our results report for the first time an efficient pipeline for the genetic improvement of fungal BCAs based on genome shuffling.
650 $aBiological control
650 $aGenetics
650 $aGenome
650 $aRoot rot
650 $aSarocladium oryzae
650 $aControle Biológico
650 $aMofo Branco
650 $aPatógeno
700 1 $aOLIVEIRA, M. I. de S.
700 1 $aMATEUS, J. R.
700 1 $aSELDIN, L.
700 1 $aSILVA-LOBO, V. L.
700 1 $aFREIRE, D. M. G.
773 $tBiological Control$gv. 152, 104460, 2021.
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