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Registro Completo |
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
06/01/2009 |
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Data da última atualização: |
06/01/2009 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
GUADAGNIN, J. P.; RODRIGUES, L. R.; BEGNINI, J. C.; BUZZETTI, D.; CASTRO, R. L.; EMYGDIO, B. M.; MONTAGNER, D.; PORTO, M. P.; POSSA, F.; RADÜNZ, L.; ROMAN, P.; STORCK, L.; TRENTIN, R.; WINKLER, L. |
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Título: |
Avaliação de cultivares de milho de ciclo superprecoce para indicação no Estado do Rio Grande do Sul - 2007/2008. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: REUNIÃO TÉCNICA ANUAL DE MILHO, 53., REUNIÃO TÉCNICA ANUAL DE SORGO, 36., 2008. Pelotas. Atas e resumos... Pelotas: Embrapa Clima Temperado, 2008. 1 CD-ROM. |
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Idioma: |
Português |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00867naa a2200265 a 4500
001 1746438
005 2009-01-06
008 2008 bl uuuu u00u1 u #d
100 1 $aGUADAGNIN, J. P.
245 $aAvaliação de cultivares de milho de ciclo superprecoce para indicação no Estado do Rio Grande do Sul - 2007/2008.
260 $c2008
700 1 $aRODRIGUES, L. R.
700 1 $aBEGNINI, J. C.
700 1 $aBUZZETTI, D.
700 1 $aCASTRO, R. L.
700 1 $aEMYGDIO, B. M.
700 1 $aMONTAGNER, D.
700 1 $aPORTO, M. P.
700 1 $aPOSSA, F.
700 1 $aRADÜNZ, L.
700 1 $aROMAN, P.
700 1 $aSTORCK, L.
700 1 $aTRENTIN, R.
700 1 $aWINKLER, L.
773 $tIn: REUNIÃO TÉCNICA ANUAL DE MILHO, 53., REUNIÃO TÉCNICA ANUAL DE SORGO, 36., 2008. Pelotas. Atas e resumos... Pelotas: Embrapa Clima Temperado, 2008. 1 CD-ROM.
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Embrapa Clima Temperado (CPACT) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Rondônia. Para informações adicionais entre em contato com cpafro.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
06/01/2009 |
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Data da última atualização: |
17/04/2009 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BRITO, L. G.; OLIVEIRA, M. C. de S.; ROCHA, R. B.; SILVA NETTO, F. G.; CAVALCANTE, F. A. |
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Afiliação: |
Luciana Gatto Brito, Embrapa Rondônia; Márcia Cristina De Sena Oliveira, Embrapa Pecuária Sudeste; Rodrigo Barros Rocha, Embrapa Rondônia; Francelino Goulart da Silva Netto, Embrapa Rondônia; Francisco Aloísio Cavalcante, Embrapa Acre. |
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Título: |
Estudo da divergência genética em linhagens brasileiras de anaplasma marginale através da utilização do gene da proteína principal de superfície MSP1a. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 15.; SEMINÁRIO DE PARASITOLOGIA VETERINÁRIA DOS PAÍSES DO MERCUSUL, 2., 2008, Curitiba. Anais... Curitiba: CBPV, 2008. |
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Idioma: |
Português |
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Notas: |
Meta 2008. |
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Conteúdo: |
Bovine anaplasmosis, caused by rickettsia Anaplasma marginale, represent one of the most important diseases ofruminants worldwide, causing significant economic losses in Brazil. This pathogen can be transmitted byRhipicephalus (Boophilus) microplus infected tick; by biting flies or by blood-contaminated fomites. The major surface proteins (msp) are involved in host-pathogen and tick-pathogen interactions and have been used as markers in A. marginale characterization and genetics studies In this study were characterized A. marginale strains obtained from naturally infected cattle from Rondônia, Acre and São Paulo State. The msp1 gene was amplified from A. marginale DNA extracted from erythrocytes by PCR in a 50 µl volume of PCR MasterMix (Promega, USA). Amplified fragments were purified and used directly for sequencing . The nucleotide and amino acid sequences of A. marginale msp1 were used for sequence alignment and genetics analysis. Multiple sequence alignment was performed using the Clustal X software. Nucleotides were coded as unordered, discrete characters with five possible character-states; A, C, G, T, or N, and gaps were coded as missing data. After a first proceeding and removal of the regions without genetic information, was carried the definitive alignment. Searches for the most parsimonious tree employed the branch and bound. The stability of the inferred topology was assessed via bootstrap analysis.. Through alignment analysis of the slighter variable region of msp1a gene, five rickettsia isolated types were identified in the A. marginale populations from São Paulo, Rondônia and Acre states. These results indicates that the strains of Acre state were less similar with São Paulo sample and not have divergence within strains obtained of samples provided MenosBovine anaplasmosis, caused by rickettsia Anaplasma marginale, represent one of the most important diseases ofruminants worldwide, causing significant economic losses in Brazil. This pathogen can be transmitted byRhipicephalus (Boophilus) microplus infected tick; by biting flies or by blood-contaminated fomites. The major surface proteins (msp) are involved in host-pathogen and tick-pathogen interactions and have been used as markers in A. marginale characterization and genetics studies In this study were characterized A. marginale strains obtained from naturally infected cattle from Rondônia, Acre and São Paulo State. The msp1 gene was amplified from A. marginale DNA extracted from erythrocytes by PCR in a 50 µl volume of PCR MasterMix (Promega, USA). Amplified fragments were purified and used directly for sequencing . The nucleotide and amino acid sequences of A. marginale msp1 were used for sequence alignment and genetics analysis. Multiple sequence alignment was performed using the Clustal X software. Nucleotides were coded as unordered, discrete characters with five possible character-states; A, C, G, T, or N, and gaps were coded as missing data. After a first proceeding and removal of the regions without genetic information, was carried the definitive alignment. Searches for the most parsimonious tree employed the branch and bound. The stability of the inferred topology was assessed via bootstrap analysis.. Through alignment analysis of the slighter variable region of ... Mostrar Tudo |
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Palavras-Chave: |
Anaplasma marginele; Estudo genético advergência. |
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Thesagro: |
Gene; Proteína. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02650naa a2200229 a 4500
001 1709264
005 2009-04-17
008 2008 bl --- 0-- u #d
100 1 $aBRITO, L. G.
245 $aEstudo da divergência genética em linhagens brasileiras de anaplasma marginale através da utilização do gene da proteína principal de superfície MSP1a.
260 $c2008
500 $aMeta 2008.
520 $aBovine anaplasmosis, caused by rickettsia Anaplasma marginale, represent one of the most important diseases ofruminants worldwide, causing significant economic losses in Brazil. This pathogen can be transmitted byRhipicephalus (Boophilus) microplus infected tick; by biting flies or by blood-contaminated fomites. The major surface proteins (msp) are involved in host-pathogen and tick-pathogen interactions and have been used as markers in A. marginale characterization and genetics studies In this study were characterized A. marginale strains obtained from naturally infected cattle from Rondônia, Acre and São Paulo State. The msp1 gene was amplified from A. marginale DNA extracted from erythrocytes by PCR in a 50 µl volume of PCR MasterMix (Promega, USA). Amplified fragments were purified and used directly for sequencing . The nucleotide and amino acid sequences of A. marginale msp1 were used for sequence alignment and genetics analysis. Multiple sequence alignment was performed using the Clustal X software. Nucleotides were coded as unordered, discrete characters with five possible character-states; A, C, G, T, or N, and gaps were coded as missing data. After a first proceeding and removal of the regions without genetic information, was carried the definitive alignment. Searches for the most parsimonious tree employed the branch and bound. The stability of the inferred topology was assessed via bootstrap analysis.. Through alignment analysis of the slighter variable region of msp1a gene, five rickettsia isolated types were identified in the A. marginale populations from São Paulo, Rondônia and Acre states. These results indicates that the strains of Acre state were less similar with São Paulo sample and not have divergence within strains obtained of samples provided
650 $aGene
650 $aProteína
653 $aAnaplasma marginele
653 $aEstudo genético advergência
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aROCHA, R. B.
700 1 $aSILVA NETTO, F. G.
700 1 $aCAVALCANTE, F. A.
773 $tIn: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 15.; SEMINÁRIO DE PARASITOLOGIA VETERINÁRIA DOS PAÍSES DO MERCUSUL, 2., 2008, Curitiba. Anais... Curitiba: CBPV, 2008.
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