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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Pecuária Sudeste. |
Data corrente: |
17/12/2014 |
Data da última atualização: |
09/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; LEAL, C. L. V.; CALLADO, M. del; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. |
Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL. |
Título: |
Chemically induced enucleation of activated bovine oocytes: chromatin and microtubule organization and production of viable cytoplasts. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Zygote, v. 23, n. 6, p. 852-862, 2015. |
DOI: |
https://doi.org/10.1017/S0967199414000537 |
Idioma: |
Inglês |
Conteúdo: |
As the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6–70.0% and blastocyst yield of 15.5–24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos. MenosAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic deve... Mostrar Tudo |
Palavras-Chave: |
Bovine; Chemically induced enucleation; Microtubule; Nuclear transfer. |
Thesaurus Nal: |
chromatin. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal W Química e Física |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1018303/1/Chemically-induced-enucleation-of-activated-bovine-oocytes.pdf
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Marc: |
LEADER 02607naa a2200277 a 4500 001 2018303 005 2024-02-09 008 2015 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1017/S0967199414000537$2DOI 100 1 $aSARAIVA, N. Z. 245 $aChemically induced enucleation of activated bovine oocytes$bchromatin and microtubule organization and production of viable cytoplasts.$h[electronic resource] 260 $c2015 520 $aAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6–70.0% and blastocyst yield of 15.5–24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos. 650 $achromatin 653 $aBovine 653 $aChemically induced enucleation 653 $aMicrotubule 653 $aNuclear transfer 700 1 $aOLIVEIRA, C. S. 700 1 $aLEAL, C. L. V. 700 1 $aCALLADO, M. del 700 1 $aVANTINI, R. 700 1 $aMONTEIRO, F. M. 700 1 $aNICIURA, S. C. M. 700 1 $aGARCIA, J. M. 773 $tZygote$gv. 23, n. 6, p. 852-862, 2015.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
22/03/1994 |
Data da última atualização: |
15/01/2007 |
Autoria: |
HOFFMANN-CAMPO, C.B.; MARTINS, C. S.; TOLEDO, J. F. F.; KIIHL, R. A. S.; MAZZARIN, R. M.; OLIVEIRA, M. C. N. de. |
Afiliação: |
EMBRAPA-CNPSo. Londrina, PR. |
Título: |
Teste de metodologia para avaliacao de resistencia de genotipos de soja a percevejos. |
Ano de publicação: |
1988 |
Fonte/Imprenta: |
In: EMBRAPA. Centro Nacional de Pesquisa de Soja (Londrina, PR). Resultados de pesquisa de soja 1986/87. Londrina, 1988. |
Páginas: |
p.93-102. |
Série: |
(EMBRAPA-CNPSo. Documentos, 28). |
Idioma: |
Português |
Palavras-Chave: |
Brasil; Cultivar; Insect; Parana; Pest; Resistance; Soybean; Variety. |
Thesagro: |
Euschistus Heros; Inseto; Nezara Viridula; Piezodorus Guildinii; Praga; Resistência; Soja. |
Thesaurus NAL: |
Brazil. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01070naa a2200385 a 4500 001 1453112 005 2007-01-15 008 1988 bl uuuu u00u1 u #d 100 1 $aHOFFMANN-CAMPO, C.B. 245 $aTeste de metodologia para avaliacao de resistencia de genotipos de soja a percevejos. 260 $c1988 300 $ap.93-102. 490 $a(EMBRAPA-CNPSo. Documentos, 28). 650 $aBrazil 650 $aEuschistus Heros 650 $aInseto 650 $aNezara Viridula 650 $aPiezodorus Guildinii 650 $aPraga 650 $aResistência 650 $aSoja 653 $aBrasil 653 $aCultivar 653 $aInsect 653 $aParana 653 $aPest 653 $aResistance 653 $aSoybean 653 $aVariety 700 1 $aMARTINS, C. S. 700 1 $aTOLEDO, J. F. F. 700 1 $aKIIHL, R. A. S. 700 1 $aMAZZARIN, R. M. 700 1 $aOLIVEIRA, M. C. N. de 773 $tIn: EMBRAPA. Centro Nacional de Pesquisa de Soja (Londrina, PR). Resultados de pesquisa de soja 1986/87. Londrina, 1988.
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