Data/hora: 22/09/2024 19:44:17

Biblioteca(s): 	Embrapa Arroz e Feijão.	
Data corrente: 	16/06/2010	
Data da última atualização: 	02/09/2010	
Autoria: 	OLIVEIRA, M. B.; NASCIMENTO, L. B.; JUNIOR, M. L.; PETROFEZA, S.	
Afiliação: 	M. B. OLIVEIRA, UNIVERSIDADE FEDERAL DE GOIÁS; L. B. NASCIMENTO, UNIVERSIDADE FEDERAL DE GOIÁS; MURILLO LOBO JUNIOR, CNPAF; S. PETROFEZA, UNIVERSIDADE FEDERAL DE GOIÁS.	
Título: 	Characterization of the dry bean polygalacturonase-inhibiting protein (PGIP) gene family during Sclerotinia sclerotiorum (Sclerotiniaceae) infection.	
Ano de publicação: 	2010	
Fonte/Imprenta: 	Genetics and Molecular Research, v. 9, n. 2, p. 994-1004, 2010.	
Idioma: 	Inglês	
Conteúdo: 	Polygalacturonase-inhibiting proteins are leucine-rich repeat proteins that inhibit fungal endopolygalacturonases. The interaction of polygalacturonase-inhibiting protein with endopolygalacturonases limits the destructive potential of endopolygalacturonases and may trigger plant defense responses induced by oligogalacturonides. We examined the expression of fungal pg and plant Pvpgip genes in bean (Phaseolus vulgaris) stems infected with Sclerotinia sclerotiorum to determine whether any of them are associated with the infection process. Transcriptional analysis was carried out by means of semi-quantitative reverse transcription PCR or real-time PCR. The sspg1 gene was highly expressed during infection; sspg3 was regulated during the later phases of infection; sspg5 was more uniformly expressed during infection, whereas sspg6 was only weakly expressed. During the course of infection, Pvpgip1 transcripts were not detected at early stages, but they appeared 72 h post-inoculation. High levels of Pvpgip2 expression were observed during the initial phase of infection; the transcript peaked by 48 h post-inoculation and declined by 72 h post-inoculation. Pvpgip3 expression increased strongly at 96 h post-inoculation. Pvpgip4 was constantly present from 24 h post-inoculation until the end of the experiment. However, we detected higher levels of the Pvpgip4 transcript in the necrotic lesion area than in plants that had been mechanically wounded. Remarkably, only Pvpgip4 appeared to be moderately induced by mechanical wounding. These results provide evidence that endopolygalacturonases contribute to the infection process during host colonization by promoting the release of plant cell oligogalacturonides, which are powerful signaling molecules and may also activate plant defenses, such as polygalacturonase-inhibiting proteins.	
Palavras-Chave: 	Endopolygalacturonase; Polygalacturonase-inhibiting protein.	
Thesagro: 	Doença fúngica; Feijão; Mofo branco; Phaseolus vulgaris; Sclerotinia sclerotiorum.	
Categoria do assunto: 	F Plantas e Produtos de Origem Vegetal	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/18863/1/gmr776.pdf	
Registro original: 	Embrapa Arroz e Feijão (CNPAF)	
Exemplares: 	CNPAF - (29918-1) - UPC - AP - DD - 2010.012 -  - 2010.012 - DIS