Data/hora: 14/06/2024 02:56:13

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	01/02/2016	
Data da última atualização: 	01/02/2024	
Autoria: 	CASTRO, R. de J. C.; SOUZA SOBRINHO, F. de; GAMA, M. A. S. da; SATO, R. T.; LIMA, L. L.; OLIVEIRA, M. A. L. de.	
Afiliação: 	Renata de Jesus Coelho Castro, UFJF; FAUSTO DE SOUZA SOBRINHO, CNPGL; MARCO ANTONIO SUNDFELD DA GAMA, CNPGL; Renata Takabayashi Sato, UFJF; Larissa Lavorato Lima, UFJF; Marcone Augusto Leal de Oliveira, UFJF.	
Título: 	A Rapid Method for Determination of the Main Conjugated Linoleic Acid Precursors (C18:2 n-6 and C18:3 n-3) in Forage by Capillary Zone Electrophoresis with Ultraviolet Detection Using Gas Chromatography with Flame Ionization Detection as a Comparative Method.	
Ano de publicação: 	2015	
Fonte/Imprenta: 	Journal of AOAC International, v. 98, n. 6, p. 1591-1597, 2015.	
Idioma: 	Inglês	
Conteúdo: 	Abstract: A rapid method has been proposed for determination of the main conjugated linoleic acid precursors such as linoleic (C18:2 n-6) and linolenic (C18:3 n-3) acids in forages by capillary zone electrophoresis (CZE) with direct UV detection at 200 nm. Among the fatty acids found in forages, C18:2 n-6 and C18:3 n-3 have received particular attention due to their roles as precursors for the synthesis of conjugated linoleic acid, a class of health-enhancing compounds that is predominantly found in dairy products. The electrolyte background consisted of 12.0 mmol/L tetraborate buffer (pH 9.2) added to 12.0 mmol/L Brij 35(®), 17% acetonitrile, and 33% methanol. Under the optimized conditions, the baseline separation of C18:2 n-6 and C18:3 n-3 was achieved within 4 min. The CZE-UV method was compared to GC with a flame ionization detector, which is the American Oil Chemists' Society (AOCS 996.06) official method for fatty acid analysis. The methods did not show any evidence of significant differences within 95% confidence interval (P > 0.05). The CZE-UV method was successfully applied to the analysis of 80 genotypes of Brachiaria ruzizienses clones submitted to a genetic improvement program in agricultural research.	
Palavras-Chave: 	CLA; CZE-UV method; Fatty acids in forages.	
Thesaurus Nal: 	conjugated linoleic acid.	
Categoria do assunto: 	F Plantas e Produtos de Origem Vegetal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (22305-1) - UPC - AP - DD -  -  -  - DIS