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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
15/02/2024 |
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Data da última atualização: |
15/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BATISTA, R. I. T. P.; DIAS, J. H.; GONÇALVES, J. D.; VERGANI, G. B.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. |
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Afiliação: |
RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense; JENNIFFER HAUSCHILDT DIAS, Universidade Federal de Viçosa; JOEDSON DANTAS GONÇALVES, Universidade Estadual Paulista; GABRIEL BRUN VERGANI, Universidade Estadual Paulista; MARIA EMILIA FRANCO OLIVEIRA, Universidade Estadual Paulista; JOANNA MARIA GONÇALVES SOUZA-FABJAN, Universidade Federal Fluminense; JEFERSON FERREIRA DA FONSECA, CNPC. |
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Título: |
The presence of estradiol benzoate in the cervical relaxation treatment for non-surgical embryo collection does not impair embryonic morphological quality, cryosurvival, and gene expression profile. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Theriogenology, v. 218, p. 208-213, Feb. 2024. |
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DOI: |
10.1016/j.theriogenology.2024.01.038. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Non-surgical embryo recovery (NSER) is usually preceded by a cervical relaxation in ovine donors, based on estradiol benzoate (EB), prostaglandin (PGF), and oxytocin (OT). However, it is hypothesized that, due to poorly understood mechanisms, EB can result in embryotoxic actions. To evaluate this, 20 min before NSER superovulated sheep were induced to cervical relaxation with 0.0 (G0.0), 0.5 (G0.5), or 1.0 mg (G1.0) of EB associated with 37.5 μg of PGF 16 h before NSER and 50 IU of OT. In doing so, the efficiency and duration of the NSER procedure showed no compromise (P > 0.05). Additionally, the presence of EB did not affect (P > 0.05) the embryo's morphological quality, the development dynamics, or the abundance of transcripts associated with embryonic quality (OCT4 and NANOG), cellular stress (HSP90 and PRDX1), and apoptosis (BCL2 and BAX). A similar result (P > 0.05) was also observed when comparing embryonic cryosurvival at 24 (52.0, 52.0, and 54.0) and 48 h (60.0, 54.0, and 58.0) of in vitro culture (G0.0, G0.5, and G1.0, respectively). Thus, we can conclude that EB use does not compromise embryonic quality and cryoresistance. |
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Palavras-Chave: |
Cervical dilation; Dosage effects; Drug effects; Embryo survival; Expressão gênica; Gene expression profiling; NSER; Sobrevivência embrionária. |
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Thesagro: |
Criopreservação; Estradiol; Genética Animal; Genética Molecular; Ovelha; Ovino; Progesterona; Reprodução; Transferência de Embrião. |
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Thesaurus NAL: |
Animal genetics; Cryopreservation; Embryo transfer; Ewes; Females; Gene expression; Molecular genetics; Oxytocin; Prostaglandins; Reproduction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02785naa a2200541 a 4500
001 2161981
005 2024-02-15
008 2024 bl uuuu u00u1 u #d
024 7 $a10.1016/j.theriogenology.2024.01.038.$2DOI
100 1 $aBATISTA, R. I. T. P.
245 $aThe presence of estradiol benzoate in the cervical relaxation treatment for non-surgical embryo collection does not impair embryonic morphological quality, cryosurvival, and gene expression profile.$h[electronic resource]
260 $c2024
520 $aAbstract: Non-surgical embryo recovery (NSER) is usually preceded by a cervical relaxation in ovine donors, based on estradiol benzoate (EB), prostaglandin (PGF), and oxytocin (OT). However, it is hypothesized that, due to poorly understood mechanisms, EB can result in embryotoxic actions. To evaluate this, 20 min before NSER superovulated sheep were induced to cervical relaxation with 0.0 (G0.0), 0.5 (G0.5), or 1.0 mg (G1.0) of EB associated with 37.5 μg of PGF 16 h before NSER and 50 IU of OT. In doing so, the efficiency and duration of the NSER procedure showed no compromise (P > 0.05). Additionally, the presence of EB did not affect (P > 0.05) the embryo's morphological quality, the development dynamics, or the abundance of transcripts associated with embryonic quality (OCT4 and NANOG), cellular stress (HSP90 and PRDX1), and apoptosis (BCL2 and BAX). A similar result (P > 0.05) was also observed when comparing embryonic cryosurvival at 24 (52.0, 52.0, and 54.0) and 48 h (60.0, 54.0, and 58.0) of in vitro culture (G0.0, G0.5, and G1.0, respectively). Thus, we can conclude that EB use does not compromise embryonic quality and cryoresistance.
650 $aAnimal genetics
650 $aCryopreservation
650 $aEmbryo transfer
650 $aEwes
650 $aFemales
650 $aGene expression
650 $aMolecular genetics
650 $aOxytocin
650 $aProstaglandins
650 $aReproduction
650 $aSheep
650 $aCriopreservação
650 $aEstradiol
650 $aGenética Animal
650 $aGenética Molecular
650 $aOvelha
650 $aOvino
650 $aProgesterona
650 $aReprodução
650 $aTransferência de Embrião
653 $aCervical dilation
653 $aDosage effects
653 $aDrug effects
653 $aEmbryo survival
653 $aExpressão gênica
653 $aGene expression profiling
653 $aNSER
653 $aSobrevivência embrionária
700 1 $aDIAS, J. H.
700 1 $aGONÇALVES, J. D.
700 1 $aVERGANI, G. B.
700 1 $aOLIVEIRA, M. E. F.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aFONSECA, J. F. da
773 $tTheriogenology$gv. 218, p. 208-213, Feb. 2024.
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